Hariani Soekamto, Nunuk
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

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Eksplorasi Metabolit Sekunder dari Spons di Wilayah Sulawesi Selatan dan Uji Bioaktivitasnya terhadap Artemia salina Jawahir, Beddu; Hariani Soekamto, Nunuk; Rosmawaty, Rosmawaty
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.2465

Abstract

Abstract. Explorations of secunder metabolic, especially non active fraction of Clathria reinwardtii, of spons from South Sulawesi have been done. Bioactivity test against Artemia salina was carried out with Brine Shrimp Lethality Test method. Separation techniques contain of extraction, fractionation, and purification. Qualitative tests and structure elucidation on the compound obtained was based on the physical and spectroscopic data UV, IR and NMR. Two compounds which are isolated from Clathria reinwardtii are suggested as fenolic compound and steroid group i.e. β-sitosterol.Keywords : β-sitosterol, Artemia salina Leach, Clathria reinwardtii, fenolic.
Sintesis 4-Hidroksisinamamida dari Asam 4-Hidroksinanamat melalui Reaksi Esterifikasi dan Amonolisis Firdaus, Firdaus; Hariani Soekamto, Nunuk; Karim, Abdul
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.2463

Abstract

The 4-hydroxycinnamamide  is a compound  that has been isolated from the root bark of Kleinhovia hospita Linn. The compound show high toxicity to Artemia salina Leach (IC 180,53 µg) so it can be expected has cytostatic activity against P388 leukemic cells. However, the concentration of the compound in the root bark of K. hospita Linn is very low (about 1.6 ppm), and commercially the compound  is  not  ready  and  has  never  been  synthesized.  Therefore,  it  is  important  to  find  out  a synthesis method to used in prepare the compound for further examination, in particular its cytostatic activity. By this research, the 4-hydroxycinnamamide compound has been prepared from 4- hydroxycinnamic  acid  via  esterification  reaction  with  excess  ethanol  and  sulphate  acid  catalyst followed  ammonolysis  reaction  with concentrated  ammonia.  The esterification  stage  gave  ethyl 4-hidroxycinnamate   as  white  crystall  with  melting  point  133-134oC   and  34.9%  yield,  and  the ammonolysis stage gave 4-hydroxycinnamamide as white crystall with merting point 140oC and 46.1% yield. Identification of the reaction product was carried out by FTIR and NMR spectroscopy methods.Keywords: 4-hydroxycinnamamide, 4-hydroxycinnamic acid, esterification, ammonolysis
STUDY OF COMPOUNDS FROM EXTRACT OF Melochia umbellata (Houtt.) Stapf var. Degrabrata K. (PALIASA) LEAVES THAT HAS POTENTIAL AS ANTIBACTERIAL Wullur, Selfi; Firdaus, Firdaus; Natsir, Hasnah; Hariani Soekamto, Nunuk
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 8 No 1 - June 2015
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v8i1.2472

Abstract

Determination of antibacterial activity of each extract of M. umbellata (Houtt.) Stapf var. K. degrabrata leaves has been conducted against three strains bacteria Escherichia coli, Staphylococcus aureus, and Shigella dysenteriae by using amoxicillin as a positive control and DMSO as a negative control. Agar diffusion method has been used with MHA medium (Muller Hinton Agar) on variation concentrations of each extract were 2500 ppm, 5000 ppm, 10,000 ppm, and 20,000 ppm N-hexane extract was the most active extract against S. aureus with zone of inhibitions were 10.50 mm, 10.80 mm, 11.00 mm and 11.45 mm, respectively. The most active extract against S. dysenteriae was ethyl acetate extracts with zone of inhibitions were 9.36 mm, 11.55 mm, 11.58 mm and 17.70 mm, respectively. Isolated compound from n-hexane extract was first compound as white powder with a melting point of 75-77 °C which was identified as 1-Tetracosanol and second compound was obtained in the form of a yellow paste which was identified as steroid compound that had long conjugated substituent. These compounds were elucidated based on the data of IR, UV-Vis, 1H-NMR, and 13C-NMR.
Characterization of Compounds From Extract of Leaf Cloroform Melochia umbellata (Houtt.) Stapf var. Degrabrata K. and Test Antihiperglicemic Activities Imran, Imran; Hariani Soekamto, Nunuk; Usman, Hanapi
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol 10, No 2: December 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v10i2.6650

Abstract

A research on the characterization of the chloroform extract of leaves of M. umbellata (Houtt.) stapf var. degrabrata K. and activity test as antihyperglycemic has been done. This study aimed to isolate and characterize compounds from chloroform extract and determine the optimum concentration of chloroform extract in  reducing blood glucose levels. Isolation compound from chloroform extract has been done by maceration, fractionation and purification. Based on the data of IR and NMR spectroscopic, compounds isolated from this research were β-sitosterol and fatty acid compound consisting of 25 carbon atoms. The glucose tolerance test, concentration of 6% showed a decrease in glucose levels almost equivalent with drug glibenclamide as comparator.
SYNTHESIS OF SILVER NANOPARTICLES USING BIOREDUCTOR OF KETAPANG LEAF EXTRACT (Terminalia catappa) AND ITS POTENTIAL AS SUNSCREEN Irfa Apriliyanti, Payapo; Zakir, Muhammad; Hariani Soekamto, Nunuk
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol 10, No 1: June 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v10i1.6395

Abstract

Synthesis of silver nanoparticles is done by the chemical reduction method using the extract of leaves of catappa (Terminalia catappa) which acts as a reducing agent with the use of AgNO3 as the precursors. The results, based on changes in color, pH, and UV-Vis absorption shows color changes from yellow to yellow-brown with a pH of 4 indicating the formation of silver nanoparticles with a wavelength range from 412,50- 404,00 nm for the storage of the first day until the seventh day with a particle size 92,48 nm based on the results of taking the measurements of PSA. Analysis of functional groups that play a role in the synthesis using FT-IR. Characterization using XRD showed that the synthesized form a cubic crystal with a crystal size of 53,48 nm. Silver nanoparticles are used as a sunscreen with a blend of hydroxy cinnamic acid compounds. Testing activities determined sunscreen with SPF values were analyzed using a UV-Vis spectrophotometer. Results of testing the activity of sunscreen alloy nanoparticles of silver and hydroxy cinnamic acid (AHNP) with a concentration of 16 μg/mL - 20 μg/ml showed the consecutive SPF value 20,97; 24,71; 26,41; 28,07 and 31,92. Sunscreen AHNP activity increases with the concentration AHNP. Based on the research results can be concluded that the combination of ultra AHNP has a protective effect (SPF ≥ 15) against UV-B rays.