Darusman, Huda Salahuddin
Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Resistansi Escherichia coli terhadap Kolistin dan Deteksi Gen Mobilized Colistin Resistance-1 pada Ayam Pedaging Akibat Pemberian Kolistin Sulfat Palupi, Maria Fatima; Maheshwari, Hera; Darusman, Huda Salahuddin; Sudarnika, Etih; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.19087/jveteriner.2018.19.2.196

Abstract

Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal. 
Nutrition Value, Feed Efficiency and Species of Seagrass as a Feed of Wild Dugong (Dugong dugon) in Lingayan island, Tolitoli, Central Sulawesi Nurdin, Muhammad Ardiansyah; Suprayogi, Agik; Satyaningtijas, Aryani Sismin; Darusman, Huda Salahuddin
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 1, JANUARY 2019
Publisher : Hasanuddin University

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Abstract

Dugong are endangered herbivorous marine mammals, which one of the causes of extinction is degradation of seagrass as main feed of the dugong to survive. Aim of this study was to measure the quality of seagrass of wild dugong in Lingayan island, Central Sulawesi. The results showed proximat analysis value of Halophyla minor, Halodule uninervis and Cymodocea rotundata as seagrass species respectively showed values crude protein of 6.86%, 7.69% and 8.79%, crude fiber of 10.77%, 18.36% and 24.26 %, crude fat of  0.99%, 1.81 and 1.5%, calcium of 0.79%, 2.12% and 1.89%, phosphorus of  0.34%, 0.34% and 0.26 and gross energy of 163.4 cal / kg, 300.5 cal / kg and 319.5 cal / kg. Cymodocea rotundata is the best feed for dugong because it contains high energy of 319.5 cal/kg and high protein 8.79%,  if compared with Halophyla minor of 1622 cal/kg) and Halodule uninervis of 3014 cal/kg) furthermore Cymodocea rotundata has a fairly high crude fat content of 1.44% which will increase fat levels under the skin (subcutaneous ) dugong as insulators of changing environmental conditions. High concentrations of fiber in Cymodocea rotundata 25.26% can help retain water as long as food passes in the intestine. The levels of nutrients in Cymodocea rotundata which found in Lingayan island deserve highest nutritious feed of dugong.