Widodo, Hermawan Setyo
Faculty of Animal Science, University of Brawijaya

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Kajian keamanan pangan dan tingkat prevalensi cemaran bakterisusu di Sentra Pengembangan Sapi Perah Cilongok Syamsi, Afduha Nurus; Astuti, Triana Yuni; Widodo, Hermawan Setyo
Jurnal Ilmu-Ilmu Peternakan Vol 28, No 3 (2018): Desember
Publisher : Faculty of Animal Science, University of Brawijaya

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Abstract

The experiment was aimed to assess the food securitythrough physical properties, chemical composition and prevalence rate of milk bacterial contamination in Cilongok dairy cattle development center. Surveys and observations were conducted to obtain data on milking and milk processing applied by farmer and to obtain data on physical properties, chemical composition, and milk total bacterial count. The materialswere used farmer and milk samples taken 20 liters in total from 13 Andini Lestari 1 farmers and 7 Andini Lestrari 2 farmers. Data from the laboratory were analyzed descriptively based on the Standard Nasional Indonesia (SNI): 3141.1.2011, "t" test was used to distinguish milk quality based on physical properties, chemical composition and total bactery from both groups. The results showed that physical properties, chemical composition, and total bactery from both groups were not significantly different (P> 0.05). The conclusion of the research stated that Cilongok Dairy Cattle Development Center produces milk that is suitable for consumption based on physical and chemical composition in accordance with SNI standard: No.3141.1.2011 that showed low prevalence of bacterial contamination.
Mengidentifikasi Peptida Bioaktif Angiotensin Converting Enzyme-inhibitor (ACEi) dari Kasein β Susu Kambing dengan Polimorfismenya Melalui Teknik In Silico Widodo, Hermawan Setyo; Murti, Tridjoko Wisnu; Agus, Ali; Widodo, Widodo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 4 (2018): November 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

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Abstract

Susu kambing memiliki komponen protein salah satunya protein β dan secara umum terjadi polimorfisme pada level protein. Perubahan urutan asam amino akibat polimorfisme memungkinkan adanya potensi dihasilkannya peptida bioaktif penghambat enzim pengubah angiotensin (ACEi). Penelitian ini bertujuan untuk menyaring peptida bioaktif yang berpotensi sebagai ACEi dari kasein β kambing beserta polimorfismenya. Penelitian ini dilakukan dengan teknik in silico terhadap sekuen kasein β kambing serta struktur tiga dimensi human testicular ACE. Langkah yang dilakukan dalam penelitian ini meliputi simulasi pemotongan peptida dengan enzim pencernaan (pepsin, tripsin dan kimotripsin), peninjauan karakteristik peptida lalu simulasi docking ligan-reseptor. Tampilan parameter Lipinski’s Rule of Five (Ro5), bioaktivitas dan energi afinitas dipertimbangkan untuk memilih peptida bioaktif. Hasil yang didapat menunjukkan bahwa ditemukan peptida bioaktif yakni INK (Ile-Asp-Lys) yang memiliki kemampuan hampir setara dengan lisinopril (afinitas energi -8,2kkal/mol vs. -8,3kkal/mol). Peptida INK dapat ditemukan dari hasil hidrolisis dari alel A, C, D dan E, sehingga polimorfisme tidak menyebabkan perbedaan produksi peptida bioaktif. Kesimpulan yang dapat diambil yakni kasein β susu kambing jika dicerna dengan enzim pencernaan dapat menghasilkan peptida bioaktif ACEi yakni INK.Identification of Angiotensin Converting Enzyme-inhibitor (ACEi) Bioactive Peptide from Goat Milk β-Casein with It's Polymorphism by In Silico TechniqueAbstractPolymorphism eventually may be occurred at the protein level. Changes in the amino acid sequence due to polymorphism may exhibit a potential action to generate of the angiotensin-converting enzyme inhibitors (ACEi) bioactive peptide. This study is aimed to assess bioactive peptides that have a great potent value as ACEi from goat β casein along with its polymorphism. The research was done by in silico technique on goat β-casein sequence and three-dimensional structure human testicular ACE. Peptide-cutting simulations with digestive enzymes (pepsin, trypsin and chymotrypsin), peptide properties review, then ligand-receptor docking simulations was applied in this research. Appearance of Lipinski's Rule of Five (Ro5), bioactivity and affinity energy were considered for selecting bioactive peptides. The results show that bioactive peptide found as INK (Ile-Asp-Lys) which had similar ability as lisinopril (energy affinity –8.2kcal/mol vs. –8.3kcal/mol). The INK peptides could be found from the hydrolysis resulted in alleles A, C, D and E, therefore polymorphism did not affect the differences of production of bioactive peptides. A conclusion, processed goat milk β casein with digestive enzymes could produce ACEi of INK as bioactive peptide.