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Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

BTM 11 is unknown species of microalgae, but has active compounds that can inhibit viruses. One of proteins produced by microalgae is a lectin. Lectin is a carbohydrate-binding protein found in various microalgae that show antiviral and antibacterial activity. The purpose of this study was to perform identification of the species of microalgae BTM 11, isolation, characterization, and assay of lectin inhibitory activity. Microalgae BTM 11 was identified to have homology with Cyanobacterium 99% and Geitlerinema sp 98%. Lectin of microalgae BTM 11 was isolated by ammonium sulfate precipitation of 75% with a molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and was unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be a alternative to antiviral and antibacterial proteins.
Pemanfaatan Kacang-Kacangan sebagai Bahan Baku Sumber Protein Untuk Pangan Darurat The Utilization of Beans as Protein Source for Emergency Food Ekafitri, Riyanti; Isworo, Rhestu
JURNAL PANGAN Vol 23, No 2 (2014): PANGAN
Publisher : Perum BULOG

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Abstract

Indonesia memiliki banyak jenis kacang-kacangan seperti kacang kedelai, kacang hijau, kacang koro pedang dan kacang kecipir, yang dapat dimanfaatkan menjadi olahan pangan. Penelitian ini bertujuan untuk memanfaatkan jenis kacang-kacangan tersebut dalam bentuk food bar sebagai pangan darurat Penelitian diawali dengan karakterisasi bahan baku pembuatan food bar. Selanjutnya dilakukan perhitungan perkiraan energi untuk menetapkan formulasi produk, yang dilanjutkan dengan pembuatan food bar serta analisa produk. Karakterisasi bahan baku menunjukkan kadar protein tepung kacang kedelai, kacang hijau, kacang koro, dan kacang kecipir berturut-turut 37,58; 23,25; 31,40; dan 41,57 persen. Perbedaan kandungan protein pada setiap jenis tepung kacang-kacangan mengakibatkan jumlah yang ditambahkan pada pembuatan food bar berbeda-beda. Pemakaian tepung kacang kedelai, tepung kacang hijau, tepung kacang koro pedang, dan tepung kacang kecipir berturut-turut adalah 27,50 persen, 41,63 persen, 3,38 persen, dan 25,13 persen. Food bar yang dihasilkan dari penggunaan bahan baku tersebut sesuai dengan kriteria pangan darurat. Food bar berbahan baku kacang-kacangan yang dihasilkan memiliki energi dari protein sebesar 10,22 - 11,33 persen, energi dari lemak sebesar 35,45 – 41,40 persen, dan energi dari karbohidrat sebesar 49,56 – 51,83 persen.Indonesia has many types of beans such as soybeans, mungbeans, jack beans, and winged beans. These beans can be utilized as processed food. This research aims to utilize these types of beans as food bar for emergency food. The study begins with the characterization of the raw material for food bar. After that calculating the estimated energy product to arrange the formulations, making food bar and finally analyzing the products. Characterization of beans flour shows that protein levels of soybean flour, mungbeans flour, jack bean flour and winged bean flour are respectively 37,58; 23,25; 31,40; and 41,57 persen. The distinction of protein on bean flour makes the amount added on food bar process distinct as well. The use soy bean flour, mung bean flour, jack bean flour, and winged bean flour in a row is 27.50, 41.63%, 3.38% and 25.13 %. Food bar produced from the use of those raw materials is in accordance with the criteria proficiency level of emergency food. Those food bar products have energy from protein of 10,22 to 11,33 percent, energy from fat of 35,45 - 41,40 percent, and energy from carbohydrates of 49,56 - 51,83 percent.