Trismawanti, Ike
Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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CAROTENOID-ENRICHED DIET FOR PRE-MATURATION STAGE OF POND-REARED TIGER SHRIMP, Penaeus monodon PART I. THE EFFECTS ON GROWTH, PIGMENTATION AND WHOLE BODY NUTRIENT CONTENT Laining, Asda; Trismawanti, Ike; Kamaruddin, Kamaruddin; Makmur, Makmur
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.297 KB) | DOI: 10.15578/iaj.12.2.2017.59-66

Abstract

 Carotenoids, besides as a natural pigment, may have vital roles in the growth of crustacean. The aim of this study was to clarify the influence of combined carotenoids given since pre-maturation stage on the growth performances, pigmentation and biochemical composition of the whole body of pond-reared tiger shrimp, Penaeus monodon. Two experimental diets were supplemented with or without carotenoid mixture consisting of astaxanthin, canthaxanthin and other carotenoids contained in Spirulina. The carotenoid mixture was supplemented in the commercial diet normally used as a starter feed for tiger shrimp, re-pelleted and fed to tiger shrimp with an initial body weight of 31.7±1.3 g. Shrimp were stocked in four 1,000 m2 concrete ponds with a density of 1 shrimp/m2 and fed until the shrimp reached maturation stage (broodstock size). Variables observed were growth performances and pigmentation properties during the pre-maturation stage and total carotenoid content in several tissues of the female broodstock after being fed with the diets until maturation stage. After 16 weeks, shrimp fed with carotenoid-enriched diet (PC) diet produced significantly (P<0.05) higher biomass than the diet without the enrichment (PO). The color of raw shrimp fed with PC diet was darker with greenish-brown compared to shrimp fed PO diet which was greenish blue. The visual appearances of 3-min steamed shrimp produced the color of red-orange for shrimp fed carotenoid compared to orange-yellow for control PO. The total carotenoid content in the whole body of shrimp fed PC diet were significantly (P<0.05) enhanced compared to control PO diet which was 42.8 ±5.8 and 55.8± 5.1 µg/g for PO and PC diet, respectively. Supplemental carotenoid in the pre-maturation diet increased the biomass production from 23.1±1.9 kg to 30.2 ±0.1 kg and enhanced the color of the shrimp which was in line with carotenoid content in the whole body of pond-reared tiger shrimp.
SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI Trismawanti, Ike; Nawang, Agus; Laining, Asda
Media Akuakultur Vol 13, No 2 (2018): (December, 2018)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1599.908 KB) | DOI: 10.15578/ma.13.2.2018.67-74

Abstract

Penggunaan induk pada pembenihan udang windu pada dasarnya hanya untuk periode yang singkat dan selanjutnya induk udang windu diapkir baik betina maupun jantan. Tujuan penelitian ini untuk mengevaluasi efek injeksi hormon kombinasi salmon gonadotrophin releasing hormone analogue (sGnRH-a) dan domperidone terhadap karakteristik spermatofor induk udang windu, Penaeus monodon yang telah diapkir dari unit pembenihan tanpa ablasi tangkai mata. Dua tahap penelitian dilakukan pada udang windu jantan yang diapkir dari unit pembenihan. Tahap pertama terdiri atas dua dosis 0,1 (OV-1) dan 0,2 (OV-2) mL/kg bobot udang dan ablasi mata (AB) sebagai kontrol. Pada tahap ke-2, dosis yang diaplikasikan adalah dosis terbaik dari tahap ke-1 (OV-3) dan ablasi sebagai kontrol (AB). Induk jantan apkir ditebar masing-masing dengan kepadatan 14 ekor untuk tahap ke-1 dan 12 ekor pada tahap kedua. Injeksi dilakukan tiga kali dengan interval satu minggu untuk kedua percobaan tersebut. Seminggu setelah suntikan terakhir, induk jantan diberi kejutan listrik untuk mengeluarkan spermatofornya. Pada percobaan pertama, jumlah induk jantan yang mengeluarkan spermatofor pada maturasi dan rematurasi pertama lebih banyak ditemukan pada perlakuan OV-2 daripada OV-1 dan AB. Induk jantan pada perlakuan OV-3 pada tahap ke-2 menghasilkan lebih banyak spermatofor (58,3%) dibandingkan dengan AB (50%). Hubungan antara bobot badan dan bobot spermatofor untuk total data pada tahap ke-1 sifatnya linier dengan nilai r= 0,74 sedangkan hubungan antara bobot spermatofor dan jumlah spermatozoa sifatnya tidak linier dengan nilai r= 0.14. Induk apkiran jantan udang windu masih dapat memproduksi spermatofor melalui stimulasi injeksi hormon sGnRH-a pada dosis 0,2 mL/kg bobot udang relatif lebih banyak dibandingkan ablasi.Broodstocks in black tiger hatcheries are commonly used for only a short period of time. Both female and male broodstocks are discarded due to reduced spawning frequencies. This recent study was an effort to explore whether discarded broodstocks could be re-maturated without eyestalk ablation. For the research purpose, a two-stage experiment was conducted on discarded male tiger shrimps to evaluate the effect of sGnRH-a injection on the shrimp reproductive performances. In the first stage, the experiment consisted of injection treatment of different doses of sGnRH-a at 0.1 (OV-1) and 0.2 (OV-2) mL/kg and eyestalk ablation (AB) as a control. For the second stage, the treatment consisted of injection of sGnRH-a optimum dose obtained from the first trial (0.2 mL/kg shrimp (OV-3) and ablation as the control (AB). The treated male stocks were then randomly placed in concrete tanks with a density of 14 males/tank for the first stage and 12 males for the second stage. The injections were carried-out three times with an interval of one week for both stages. A week after the last injection, males were electrically shocked to release their spermatophores. In the first stage, the number of males releasing spermatophores at maturation and first re-maturation was higher in OV-2 treatment than that of OV-1 and AB treatments. In the second trial, the number of males in OV-3 treatment released more spermatophore (58.3%) compared to AB which was 50%. The body and spermatophore weights correlation of the discarded tiger shrimp in the first trial followed a positive linear with an r-value of 0.74. However, the spermatophore weight and the sperm cell number was not positively correlated with an r-value of 0.14. The discarded male tiger shrimps produced a relatively higher spermatophore when induced with sGnRHa at a dose of 0.2 mL/kg than those of shrimp treated with ablation.
KELAYAKAN PENGGUNAAN PROBIOTIK RICA KEMASAN SERBUK UNTUK PEMELIHARAAN PASCA LARVA UDANG WINDU Penaeus monodon DALAM BAK TERKONTROL Tampangallo, Bunga Rante; Trismawanti, Ike; Muliani, Muliani
Media Akuakultur Vol 13, No 2 (2018): (December, 2018)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.58 KB) | DOI: 10.15578/ma.13.2.2018.91-98

Abstract

Probiotik RICA kemasan cair telah diaplikasikan pada pemeliharaan udang windu P. monodon maupun vaname Litopenaeus vannamei. Namun, probiotik kemasan cair dirasa banyak mengalami kendala dalam hal pengiriman. Alternatif bentuk kemasan yang saat ini sedang dikaji adalah bentuk kemasan serbuk. Penelitian ini bertujuan mengetahui viabilitas probiotik RICA setelah dikemas dalam bentuk serbuk, waktu penyimpanan, serta pengaruhnya terhadap sintasan dan pertumbuhan benih udang windu yang dipelihara dalam bak terkontrol. Penelitian dilakukan dalam dua tahap, yakni penepungan probiotik, pengamatan populasi probiotik, dan aplikasinya pada pemeliharaan udang windu. Wadah pemeliharaan udang windu menggunakan bak fiber volume 500 liter. Udang uji yang digunakan adalah udang windu PL-12 dengan kepadatan 200 ekor per bak. Penelitian menggunakan rancangan acak lengkap empat perlakuan tiga ulangan. Perlakuan menggunakan probiotik serbuk RICA-4 (A), (B) probiotik RICA-5, (C), probiotik RICA-1, dan (D) tanpa probiotik. Hasil penelitian menunjukkan bahwa populasi probiotik RICA setelah diserbukkan berkisar 107 sel/mL. Sintasan, bobot, dan panjang udang windu setelah dipelihara selama 48 hari dengan menggunakan probiotik RICA serbuk belum memberikan hasil yang signifikan (P>0,05), namun pemberian probiotik RICA telah meningkatkan total hemolim udang windu secara signifikan (P<0,05) dibanding kontrol. Pada aplikasi probiotik ini terjadi pertumbuhan lumut dalam bak pemeliharaan akibat penggunaan tepung kanji sebagai filler.RICA probiotics have been tested in multiple farms of black tiger shrimp P. monodon and whiteleg shrimp Litopenaeus vannamei with some promising results. However, the probiotics’ distribution in the form of liquid faces some limitations due to transportation regulations involving liquid-based substances. A powder form might be an easy and secure alternatively for the distribution of the probiotics via airplane. The purpose of the study was to observe the viability of RICA probiotics after powdering and storage as well as their influences on the survival and growth rates of black tiger shrimp reared in controlled tanks. The study was conducted in three stages which were the powdering of the probiotic, observation of the probiotics’ bacteria population, and its application in shrimp rearing. The research was designed in a completely randomized design where 200 shrimps aged PL-12 reared in each tank and applied with three different powder probiotics namely (A) RICA-4, (B) RICA-5, and (C) RICA-1 as the treatments, and (D) without adding probiotic as a control. The result showed that the probiotic population after powdering ranged around 107 cells/mL. The survival rate, body length, and weight of shrimps after 48 days of rearing showed insignificant results (P>0.05) among the treatment. However, RICA-1 had an increase in the total of hemocytes of the shrimps (P<0.05) compared to control. The emergence of moss in the containers became a constraint during the experiment.