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Segregation Analysis of Transgenic Rice Plants CV Rojolele Harboring Cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy; Sulistyowati, Yuli; Zahra, Fatimah; Nurhasanah, Ade Nena; Rahmawati, Syamsidah; S-Loedin, Inez H
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for  cry1B gene. Selection  of homozygous transgenic rice lines containing  cry1B  gene at third, fourth and fifth generation had been conducted by PCR.  The presence of  cry1B  gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice  lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for  cry1B  gene.   Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that  these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of  cry 1B  gene to yellow stem borer in naturalcondition, confined field trial in endemic area of yellow stem borer should be conducted. Keywords: bioassay, cry1B gene, transgenic rice, yellow stem borer
Penapisan Beberapa Padi Lokal dari Pulau Enggano Terhadap Ketahanan Salinitas Astuti, Dwi; Nurhasanah, Ade Nena; Nugroho, Satya; Estiati, Amy
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 2 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Astuti et al, 2017. Screening of Several Local Rice from Enggano Island to Salinity Tolerance. JLSO 6(2):134-141. Enggano Island, Bengkulu Province, has a lot of local rice germplasm that has been cultivated by people in Enggano Island that is more than 30% as farmer. Identification of local rice from Enggano Island against biotic and abiotic stresses, especially salinity stress is necessary. The identification can be used, among other things, as the information of the local rice planting that related to the rising sea level in Enggano Island which impact on the condition and the area of rice cultivation land, and as a source of finding for resistance genes against salinity stress for improvement of those local rice varieties. Aim of this study is preliminary screening of some local rice from Enggano Island to salinity stress in seed and germination stages. The analysis used Factorial Randomized Design, two factors, the first factor  is salt concentration and and the second is local variety. The salt concentrations used were 0 millimolar NaCl, 250 millimolar NaCl, NaCl 275 millimolar and NaCl 300 millimolar and the rice sample used were 11 local rice of Enggano Island, NiponBare varieties as control of rice plants, Pokali as resistant control and IR29  varieties as susceptible controls. Rice seeds that used in this test are seed in seed stage and germination stage of 10-14 days old. This preliminary screening shows three local genotype of Enggano Island that tolerance to Salinity.
Respon Pertumbuhan Padi Mutan Insersi pada Kondisi Nitrogen Rendah Nurhasanah, Ade Nena; Nugroho, Satya
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 1 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Nurhasanah et al, 2017. Response Rice Mutant Insertion Growth in the Low Nitrogen Conditions. JLSO 6(1):49-58.Nitrogen is an essential fertilizer for growing rice because N needed higher than other nutrients. Therefore, many farmers apply a large amount of nitrogen fertilizer to obtain high production. Unfortunately, the plant not utilized much and only 50-60 % of the total N was applied which able to used. It would be lost through a combination of leaching, surface run-off, denitrification, volatilization and microbial consumption. Thus, the large amount N fertilizer application increase input cost and damage the environment. To build sustainable agriculture, the screening of varieties or mutant which is capable of using N efficiently is necessary. In addition, knowledge of N regulation and mechanisms in plants needs to be exploited for efficient plant development in the use of N. This study aims to look at the response of mutant rice plants in the low N conditions and obtain mutant rice plant candidates which is efficiently in using N. Seventen mutan insertion and Niponbare as wildtype are used in this experiment. Rice mutant plants were grown hydroponically inYoshida solution with different N concentration. Under normal conditions the N concentration was 1.14 mM/L and in the low N concentration was about 0.114 mM./L. Parameters was measured in this study were plant height, root length and dry weight. The results showed that plant height mutant insertion wasnot influenced by N concentration in nutrient solution. Meanwhile root lenght and dry weight plant parameter are affected by N consentration. There are mutant insertion plant that have potential and the optimum growth ability at low N concentration, especially B101. Validation on that mutants will be done in the next research.