Safika, Safika
Syiah Kuala University

Published : 2 Documents
Articles

Found 2 Documents
Search

TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT Safika, Safika; Darmawi, Darmawi; Ramadhani, Fitria Sari; Nurhaspika, Nurhaspika; Moliwati, Moliwati
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.9288

Abstract

This study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39° C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species.
Correlation of Aspergillus flavus with the Concentration of Aflatoxin B1 in Ikan Kayu Safika, Safika
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9784

Abstract

The objective of this research was to know the contamination of Aspergillus flavus in ikan kayu and the number of aflatoxin B1 (AFB1) produced. Also the corelation the number of A. flavus with AFB1. Samples were taken from traditional markets in Banda Aceh and Lhokseumawe. The sample were used to isolation and identify A. flavus also detect the present of AFB1 by ELISA (Enzym Linked ImmunosorbantAssay). The result shows that all samples show the present of A. flavus with the concentration 1 x 102-61 x 102 Cfu/g and the concentration of AFB1 between 0.9-38.7 ppb. In conclusion the present A. flavus in ikan kayu has a corelation with the production of AFB1.