Supriyadi, Adi
Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
Penurunan Temperatur Instalasi Kabel NYM 2x1.5mm2 Dengan Mengatur Sudut Penekukan Manfaluthy, Mauludi; Syukur, Muhammad; Supriyadi, Adi
TEKNIK Vol 39, No. 2 (2018): Desember 2018
Publisher : Diponegoro University

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Abstract

Salah satu faktor yang menyebabkan tingginya temperatur pada instalasi kabel listrik adalah posisi kabel yang ditekuk. Pemasangan instalasi listrik di rumah maupun pabrik tidak selalu lurus, dalam keadaan tertentu dan pada lokasi tertentu pemasangan kabel mengharuskan penekukan. Penekukan kabel yang tidak memperhitungkan sudut penekukan menghasilkan kenaikan temperature yang tinggi sehingga memungkinkan terjadinya isolasi terbakar dan kawat penghantar meleleh. Penelitian ini bertujuan untuk menurunkan temperature instalasi Kabel NYM 2 x 1,5mm2 dengan mengatur sudut penekukan. Instalasi kabel NYM 2 x 1.5mm2 dialiri arus dengan variasi 5A, 10A, 17A, 18A dan 19A dengan sudut tekukan kabel adalah pada 30o, 60o, 90o, 120o , dan kabel ditekuk balik. Pengujian menggunakan alat test Omicron CPC 100 yang mampu mengeluarkan tegangan output hingga 2kV AC dengan arus listrik sebesar 800A AC dan 400A DC. Hasil penelitian menunjukkan bahwa instalasi kabel dengan sudut penekukan yang tumpul mengurangi temperatur secara signifikan sehingga isolasi tidak terbakar dan kawat penghantar tidak meleleh. Instalasi kabel dengan sudut penekukan lancip (300) menyebabkan temperature naik secara signifikan sehingga isolasi lebih mudah terbakar.
Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.