Rosnizar, Rosnizar
Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Morphology and Parasitaemia Development of Plasmodium berghei in Balb/c Mice (Mus musculus)

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Malaria disease caused by Plasmodium parasite have symptoms that typically include fever, fatigue, vomiting and headaches. In severe cases, it can cause yellow skin, seizures, coma or death. The present study is aimed to monitoring parasitemia level and percentage of parasite morphology as parasitaemia progresses. This research used Plasmodium berghei NK strain obtained from National University of Malaysia which originally from MR4, USA. Design used in this research was completely randomized design, with 2 treatments which were mice without infection and mice with infection of P. berghei parasite. Method used in this research was staining method of thin smear of blood using Giemsa stained and observed by microscopic. Parameter of observation were morphology and development of P. berghei at different level of parasitaemia (10%, 20%, 30% and 40%). The result showed from microscopic examination of blood slides prepared from the study animals indicated that the ring form was dominant stage obtained at all different stages of infection followed by trophozoite stage. Meanwhile schizont stage was the lowest stage obtained at all different stages observed.

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Abstract

Aceh swamp buffaloes(Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloesafterthawing.This study used completelya factorial randomized design with nine treatments, and five replications.Fresh semen of the Aceh swamp buffalowere diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM(L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours.The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo(B. bubalis)after thawing.The percentage of sperm motility L120G7 (42.60 1.14);viability L120G7 (55.00 0.71);acrosome integrity L120G7 (52.00 0.71); and plasma membrane integrity L120G7(53.20 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).