Giri, I Nyoman Adiasmara
Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA Hutapea, Jhon Harianto; Andamari, Retno; Giri, I Nyoman Adiasmara; Permana, Gusti Ngurah
Jurnal Riset Akuakultur Vol 1, No 3 (2006): (Desember 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1562.994 KB) | DOI: 10.15578/jra.1.3.2006.325-336

Abstract

KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA
RESPON IMUNITAS BENIH LOBSTER, Panulirus homarus DENGAN PENGGUNAAN PROBIOTIK PADA PAKAN MOIST Haryanti, Haryanti; Sembiring, Sari Budi Moria; Sudewi, Sudewi; Widiastuti, Zeny; Giri, I Nyoman Adiasmara; Sugama, Ketut
Jurnal Riset Akuakultur Vol 12, No 1 (2017): (Maret 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.419 KB) | DOI: 10.15578/jra.12.1.2017.85-97

Abstract

Pemeliharaan benih lobster P. homarus masih menghadapi beberapa permasalahan, di antaranya infeksi penyakit bakteri (red body disease) dan mortalitas yang tinggi. Tujuan penelitian ini adalah untuk mengkaji respons imunitas benih lobster P. homarus yang diberi pakan pelet basah (moist diets) dengan penambahan probiotik. Pemeliharaan benih lobster dilakukan secara individu (1 ekor/keranjang). Lama pemeliharaan selama tiga bulan. Bobot awal puerulus P. homarus adalah 0,37 ± 0,05 g. Perlakuan meliputi pemberian pakan moist yang ditambahkan (A) ragi Saccharomyces cerevisiae, (B) kombinasi probiotik, Alteromonas sp. BY-9 dan Bacillus cereus BC, dan (C) tanpa probiotik. Respons imunitas dianalisis dengan RT-qPCR melalui tujuh gen target terkait ekspresi imunitas, setelah diuji tantang dengan Vibrio harveyi (penyebab red body disease). Hasil penelitian menunjukkan bahwa sintasan benih lobster sebesar (A) 32,22%; (B) 29,63%; dan (C) 33,33%. Pertumbuhan panjang dan bobot benih lobster tidak berbeda nyata (P>0,05). Respons imunitas benih lobster P. homarus pada perlakuan A dan B menunjukkan nilai ekspresi imun yang lebih tinggi dibandingkan dengan perlakuan C (tanpa probiotik). Ekspresi gen penyandi anti lipopolisakarida (ALFHa-1) meningkat pada (A) rata-rata sebesar 3,44 kali dan (B) 3,25 kali dibandingkan dengan perlakuan C (2,43 kali). Kelipatan ekspresi profenoloksidase (proPO) benih lobster meningkat pada perlakuan A (penambahan ragi) rata-rata sebesar 5,27 kali, sedangkan pada perlakuan B (kombinasi probiotik) sebesar 12,92 kali. Ekspresi Clotting sistem (transglutaminase, clotting protein) dan antioxidant defense mechanism (glutathione peroxidase/GPO) dan SAA juga mengalami peningkatan pada perlakuan A dan B.A number of contrains including disease infections and significant mortality have been occurring in lobster aquaculture. The aim of this research was to observe the immune response of juvenile lobster P. homarus culture fed by moist pellet supplemented with probiotic. Experimental juveniles were reared in individual system (one juvenile/basket). The experiment was conducted for three months. The treatments comprised (A) whole cell of yeast Saccharomyces cerevisiae, (B) combination of probiotics Alteromonas sp. BY-9 and Bacillus sp. BC, and (C) without probiotic as control. Initial weight of juveniles were 0.37 ± 0.05 g. Immunity responses were analyzed using seven immunity related genes expression by RT-qPCR. The results showed that the survival rate of juvenile for treatments A, B, and C were 32.22%, 29.63%, and 33.33% respectively. The weight and length gain of the juvenile were not significantly different (P>0.05) among treatments. Based on immunity related gene expression analysis, it revealed that A and B treatments have shown differences in the increament of immunity responses. Expressions of ALFHa-1 genes were increased on (A) treatment with average of 3.44 fold and (B) treatment (3.25 fold) and higher than C treatment (2.03 fold). Prophenoloxidase (ProPO) expression was increase average up to 5.27 fold on A (yeast supplementated) treatment and B (combination of probiotic) were 12.92 fold. Gene expression on Clotting system (transglutaminase, clotting protein) and antioxidant defense mechanism (glutathione peroxidase/GPO) was increased on A and B treatments.
REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra) Sembiring, Sari Budi Moria; Wibawa, Gigih Setia; Giri, I Nyoman Adiasmara; Hutapea, Jhon Harianto; Haryanti, -
Marine Research in Indonesia Vol 43 No 1 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.996 KB) | DOI: 10.14203/mri.v43i1.267

Abstract

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.
APLIKASI VAKSIN BIVALEN (VNN dan GSDIV) PADA PEMELIHARAAN LARVA Ikan Kerapu Sunu, Plectropomus leopardus Mahardika, Ketut; Mastuti, Indah; Sudewi, Sudewi; Asih, Yasmina Nirmala; Muzaki, Ahmad; Giri, I Nyoman Adiasmara
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.13.4.2018.337-346

Abstract

Beta-nodavirus sebagai agen penyebab VNN (virus nervous necrosis) dan infeksi GSDIV (grouper sleepy disease iridovirus, isolat dari genus Megalocytivirus) merupakan penyakit yang menyebabkan mortalitas yang tinggi pada larva dan juvenil ikan kerapu dan kakap di Indonesia. Pencegahan infeksi virus tersebut menjadi prioritas utama dalam budidaya ikan tersebut. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas vaksin bivalen dalam mencegah infeksi virus VNN dan GSDIV pada pemeliharaan larva ikan kerapu sunu, Plectropomus leopardus. Sebanyak 5 mL vaksin bivalen (kombinasi antara vaksin protein rekombinan VNN dan GSDIV dengan rasio 1:1 v/v) di bio-enkapsulasi ke dalam 30 liter pakan alami Rotifera dan Artemia (2 x 104 individu/mL). Aplikasi vaksin pada larva ikan kerapu sunu dilakukan melalui pakan alami Rotifera dari umur 5-24 hari dan Artemia dari umur 25-50 hari. Hasil penelitian menunjukkan bahwa vaksin bivalen tidak berpengaruh terhadap pertumbuhan dan sintasan larva kerapu sunu (panjang: 1,8-2,2 cm dan sintasan: 1,05%-4,07%). Hasil uji tantang dengan VNN dan GSDIV menunjukkan bahwa vaksin tersebut dapat menginduksi gen imunitas larva (MHC-I).Beta-nodavirus as the causative agent of VNN (viral nervous necrosis) and GSDIV infection (grouper sleepy disease iridovirus, isolate from the genus Megalocytivirus) has caused high mortality of cultured grouper and sea bass larvae and juvenile in Indonesia. The prevention of this virus infection on grouper and sea bass culture has become one of the national priority. The purpose of this research was to study the effectiveness of the bivalent vaccine in preventing VNN and GSDIV infections to seed of coral trout grouper, Plectropomus leopardus reared in hatchery. Applications of bivalent vaccine (a combination of protein recombinant VNN and GSDIV vaccine with a ratio of 1:1 v/v) were done by bio-encapsulation using the fish natural diet, Rotifera and Artemia, with a dose of 5 mL vaccine in 30 liters of natural diet (2 x 104 ind./mL). Vaccines were given once a day from the larval age of 5-24 days after hatching using Rotifera and 25-50 day after hatching using Artemia. The results showed that the bivalent vaccine did not influence the growth and survival rate of coral trout grouper larvae (ranged of total length: 1.8-2.2 cm and survival rate: 1.05%-4.07%). The challenge test with VNN and GSDIV revealed that the vaccine had positively induced gene related immunity of larvae MHC-I.