Articles

Found 3 Documents
Search

ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT Fachrial, Edy; Harmileni, Harmileni
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 5, No 1 (2018): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.827 KB) | DOI: 10.31289/biolink.v5i1.1707

Abstract

The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.
PEMBUATAN BIOPESTISIDA DARI EKSTRAK DAUN SIRSAK (Annona muricata) UNTUK PENGENDALIAN HAMA ULAT API (Setothosea asigna V.Eecke) PADA TANAMAN KELAPA SAWIT (Elaeis guineensis Jacq) Harmileni, Harmileni; Saragih, Gimelliya; Fernandez, Benny Rio; Yunianto, Yunianto
JURNAL BIOSAINS Vol 5, No 1 (2019): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v5i1.12331

Abstract

Ulat api (Setothosea asigna V.Eecke) merupakan hama pemakan daun yang terpenting di perkebunan kelapa sawit terutama di Sumatera Utara. Ulat ini dikenal sebagai ulat yang rakus yang sering menimbulkan kerugian di perkebunan kelapa sawit pada tanaman muda maupun tanaman tua. Pada perkebunan kelapa sawit, ulat pemakan daun kelapa sawit umumnya diatasi dengan menggunakan insektisida kimia sintetik yang mampu menurunkan populasi hama dengan cepat, sehingga dapat dihindari terjadinya kerusakan daun lebih lanjut. Walaupun demikian, penggunaan insektisida kimia sintetik secara kurang bijaksana telah terbukti dapat menimbulkan berbagai dampak negatif terhadap lingkungan. Tujuan penelitian ini adalah pembuatan biopestisida dari daun sirsak untuk mengendalikan hama ulat api (Setothosea asigna V.Eecke). Daun sirsak diekstrak dengan pelarut etanol 96%. Ekstrak pekat yang diperoleh diencerkan dengan akuades menjadi beberapa variasi konsentrasi yaitu 10%, 20%, 30%, 40% dan 50%. Hasil uji insektisida menunjukkan ekstrak daun sirsak 30% sudah efektif dalam mengendalikan hama ulat api. Dari hasil tersebut disimpulkan bahwa daun sirsak dapat dimanfaatkan sebagai biopestisida dalam mengendalikan hama ulat api dengan mekanisme racun kontak. 
AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA” Aloysius, Aloysius; Ulfa, Anjurniza; Situmorang, Anggita Kasih Fianti; Harmileni, Harmileni; Fachrial, Edy
BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan Vol 6, No 1 (2019): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2165

Abstract

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.