Pertiwi, Ratih Dyah
Akademi Farmasi Samarinda

Published : 3 Documents

Found 3 Documents

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL LIMBAH KULIT BUAH APEL (Malus domestica Borkh.) TERHADAP RADIKAL BEBAS DPPH (2,2-Diphenyl-1-Picrylhydrazil) Pertiwi, Ratih Dyah; Yari, Cut Ervinar; Putra, Nanda Franata
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : Akademi Farmasi Samarinda

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Recently, many plants have not been utilized properly, one of those is apple plant. There are several types of apples which are widely consumed and used as processed products, among others, as candied apples, syrup and fruit juice. The effluent from the process in the form of processed peel and pulp is not only used as a substitute for animal feed and fertilizer plants, but also used as a natural antioxidant that is needed by the human body against various free radical. Most of the people who like to consume apples prefer to discard the apple peel without using it. Apple peel contains quercetin substances, which needed to increase the levels of antioxidants. The preparation of ethanol extract from apple peels waste is used by maceration method using solvent 70% ethanol. To test the antioxidant activity towards free radical DPPH the extract was made into several concentrations, namely 10 ppm, 20 ppm, 30 ppm, 40 ppm and 60 ppm, and than mixed with a solution of DPPH with concentration of 100 ppm. The absorbance was measured using  a  spectrophotometer  UV-Visible.  Based  on  the  result  of  the  research,  the percentage  of  reduction  obtain  from  those  of  extract  concentrations  were  8.580%, 14.510%, 21.653%, 26.685% and 34,232%. From percentage reduction result, it was obtain IC50 in the amount of 87.795 ppm. Based on the IC50 values can be conclud that the ethanol extract of apple peel waste has strong antioxidant activity classification
UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus Pertiwi, Ratih Dyah; Kristanto, Joni; Praptiwi, Graha Ayu
Jurnal Ilmiah Manuntung Vol 2 No 2 (2016): Jurnal Ilmiah Manuntung
Publisher : Akademi Farmasi Samarinda

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One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria
Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin Pertiwi, Ratih Dyah; Djajadisastra, Joshita; MUTALIB, ABDUL; Pujiyanto, Anung
Publisher : Fakultas Farmasi Universitas Indonesia

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Gold nanoparticles (AuNP) are potentially developed as nanomedicine because AuNP is easily synthesized, functionalized, and biocompatible. With gum arabic as a stabilizer, vincristine was conjugated with gold nanoparticles. As a reducing agent, it used 0.02 M Natrium Boro Hidrat (NaBH4)  solution. Gold nanoparticles (AuNP) coated with conjugated gum Arabic (GA) and vincristine (VCR) were successfully synthesized and characterized. The conjugation of GA-VCR and AuNP displayed a narrow hydrodynamic particle size distribution with average size < 100 nm by TEM and  PSA (particle size analyzer). We investigated the cytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle by tetrazolium salt assay (MTT) using cancer cell line CCR-CEM. Cytotoxic activity of conjugated VCR-GA-AuNP before and after purification by Size Exclusion Chromatography (SEC), against leukemia cell line CCRF-CEM, was described by IC50 value. All formulation had a cytotoxic of activity with IC50 <20 μg/ml. The IC50 of samples against CCRF cell line were 1,026 μg/mL and  2,607 ug/mL, respectively.