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Gambaran Sitologi Large Cell Carcinoma Paru Anggraini, Dwi Rita; Feriyawati, Lita
ANATOMICA MEDICAL JOURNAL | AMJ Vol 1, No 1 (2018): Anatomica Medical Journal
Publisher : Universitas Muhammadiyah Sumatera Utara

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Abstract

AbstrakLarge cell carcinoma (LCC) merupakan suatu udifferentiated non-small cell carcinoma adalah subtipe dari kanker paru yang tidak dijumpai gambaran small cell carcinoma (SCLC), adenocarcinoma (ADC), maupun squamous cell carcinoma (SQC). Aplikasi sitologi paru yang  relevan saat ini adalah dalam mendiagnosa dan penanganan kanker paru. Sekitar 70% kanker paru didiagnosis pada stadium lanjut dan tidak dapat diobati.Pada artikel ini, dilaporkan gambaran sitologi dari LCC. Laporan kasus seorang pria usia 54 tahun yang secara klinis diduga karsinoma bronkus, kemudian dilakukan brushing bronkus. Pemeriksaan sitologi  menunjukkan kelompokan sel-sel tumor yang hiperseluler, membentuk pola syncytia-like sheets, dengan batas antar sel tidak jelas. Sel-sel tumor pleomorfik, dijumpai giant sel, inti bulat sampai irregular, beberapa dijumpai binukleasi, kromatin kasar, anak inti menonjol, sitoplasma eosinofilik. Hasil pemeriksaan sitologi suatu LCC. Prognosa umumnya buruk, reseksi komplet pada stadium I atau II, angka ketahanan hidup diperkirakan berkisar antara 0%-21%. Kata kunci : large cell carcinoma, kanker paru, undifferentiated non-small cell carcinoma,  sitologi.  AbstractLarge cell carcinoma (LCC) as an undifferentiated non-small cell carcinoma is a merely descriptive term indicating a subtype of lung cancer with no specific features of small-cell lung cancer (SCLC), adenocarcinoma (ADC) or squamous cell carcinoma (SQC). The most relevant application of lung cytology today is in the diagnosis and management of lung cancer; approximately 70% of those cancers are diagnosed at a late stage and are unresectable. We investigated the cytology smear of large cell carcinoma. The case report of a  54 years old man, clinically diagnosed with suspected carcinoma bronchus. The cytology features from brushing bronchus showed hypercellularity of cell tumor arranged in cellular aggregates or syncytia-like sheets of large cells with indistinct cell borders. The cells are extreme pleomorphisme, bizarre tumor giant cells,  nuclei round to irregular, sometimes binuclei with vesicular chromatin and prominent nucleoli, with eosinosophilic cytoplasm. The result of cytology evaluated were Large Cell Carcinoma. The prognosis was poorly, completely resection of stage I or II, the survival rate was estimated to range between  0% to 21%.  Key words: large cell carcinoma, lung cancer, undifferentiated non-small cell carcinoma,  cytology.
Perbedaan Ekpresi Imunositokimia Thyroid Transcription Factor-1 (TTF-1) pada Adenokarsinoma dan Karsinoma Sel Skuamosa dari Sikatan Bronkus Kanker Paru Feriyawati, Lita; Lubis, Nadjib Dahlan; -, Delyuzar
Majalah Patologi Indonesia Vol 24 No 1 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAK Latar belakang Karsinoma bukan sel kecil (KBSK) merupakan 85% keganasan dari seluruh kanker paru. Dalam beberapa tahun terakhir adenokarsinoma merupakan tipe KBSK yang paling dominan. Pemeriksaan sitologi sikatan bronkus dengan pewarnaan rutin papanicolaou pada beberapa keadaan mempunyai keterbatasan, terlebih pada beberapa jenis tumor paru dengan gambaran sel yang mirip. Thyroid transcription factor-1 (TTF-1) diekspresikan pada adenokarsinoma paru dan karsinoma sel kecil paru. Pemeriksaan imunositokimia TTF-1 dengan prosedur yang efisien dan efektif sebagai alat bantu pemeriksaan sitologi sikatan bronkus dapat meningkatkan keakuratan diagnosa sitologi kanker paru. Tujuan penelitian ini adalah untuk membedakan adenokarsinoma dan karsinoma sel skuamosa (KSS) dari sikatan bronkus kanker paru melalui tampilan imunositokimia TTF-1. Metode Penelitian ini merupakan penelitian analitik dengan rancangan potong lintang pada 45 sampel sitologi sikatan bronkus yang terdiri dari adenokarsinoma sebanyak 77,78% (n=35) dan KSS sebanyak 22,22% (n=12). Dilakukan pemeriksaan imunositokimia TTF-1 menggunakan antibodi TTF-1 rabbit polyclonal (klon ab136633) dengan metode labelled streptavidin biotin immunoperoxidase complex langsung pada slaid yang sebelumnya telah diwarnai dengan papanicolaou. Hasil Pada penelitian ini sebanyak 62,9% (n=22) adenokarsinoma menunjukkan tampilan positif dengan imunositokimia TTF-1, ekspresi kuat 36,3% (n=8), ekspresi sedang 50% (n=11), dan ekspresi lemah 13,6% (n=3), serta tampilan negatif 37,1% (n=13). Sampel KSS menampilkan hasil positif dengan imunositokimia TTF-1 sebanyak 30% (n=3) dengan ekspresi kuat serta tampilan negatif sebanyak 70% (n=7). Hasil uji hubungan antara hasil imunositokimia TTF-1 dengan ekspresi pada adenokarsinoma dan KSS menggunakan uji Fisher’s exact menunjukkan p-value = 0,083. Kesimpulan Pada penelitian ini didapati tampilan intensitas yang beragam pada hasil imunositokimia TTF-1 pada jenis adenokarsinoma dan KSS, dan menurut perhitungan statistik tidak bermakna. Kata kunci: adenokarsinoma, kanker paru, karsinoma sel skuamosa, sikatan bronkus, thyroid transcription factor-1. ABSTRACT Background Non-small cell carcinoma (NSCC) is 85% of all lung cancer malignancy. In recent years, adenocarcinoma is the most dominant type of NSCC. Bronchial brushings cytology examination with routine Papanicolaou staining have limitations especially to distinguish between some types of lung tumors which have similar pattern. Thyroid transcription factor-1 (TTF-1) is a transcription factor that normally found in adult thyroid and lung tissue, expressed in lung adenokarsinoma and small cell lung carcinoma. Immunocytochemistry examination TTF-1 is an efficient and effective procedures as aids bronchial brushings cytology examination that can improve the accuracy of cytological diagnosis of lung cancer. The objective of the research is to determine the differences between adenocarcinoma and squamous cell carcinoma (SCC) from bronchial brushings in lung cancer by the expression of immunocytochemistry TTF-1. Methods A cross sectional analytic study was performed at 45 brushings cytology samples consisted of 77.78% (n=35) adenocarcinoma and 22.22 % (n=12) SCC Immunocytochemistry examination using rabbit polyclonal (clone ab136633) TTF-1 antibody was perfomed by immunoperoxidase labeled streptavidin biotin complex methode directly on slides previously stained with Papanicolaou. Results In this study, 62.9% (n=22) adenocarcinoma were positive for TTF-1 with 36.3% (n=8) gave strong expression, 50% (n=11) gave moderate expression, 13.6% (n=3) gave weak expression and showed negative as 37.1% (n=13). SCC were positive for TTF-1 as 30% (n=3) with overall gave strong expression and showed negative results as 70% (n=7). The relationship between TTF-1 expression in adenocarcinoma and SCC by using Fisher 's exact test showed p-value=0.083 Conclusion This study showed that there were not differences of TTF-1 immunocytochemistry intensity between adenocarcinoma and SCC. Key words : adenocarcinoma, bronchial brushings, lung cancer, squamous cell carcinoma, thyroid transcription factor-1.
The Effect of Torbangun Leaves Tea on Msg-induced Fetal Develop-ment Disorder in Mice M.Kes, Sufitni; Feriyawati, Lita; Pane, Yunita Sari; Lelo, Aznan
Sumatera Medical Journal Vol 2 No 1 (2019): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

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Abstract

The safety of MSG on the fetus has not been confirmed yet. The objective of this research is to determine the effect of tea of torbangun leaves on fetal development disorder of mice. The present study is experimental with a Completely Randomized Design (CRD). Data obtained were then analyzed by ANOVA, with p<0,05 as the level of significant.   The comparison of mean values ± SEM increase number of live fetuses  group C1-T1 (4,60 ± 0,93; 11,40 ± 0,87), p=0,018. The comparison of mean values ± SEM decrease number of embryo re-sorption percentage group C0-C1 (00,00 ± 00,00; 36,74 ± 15,13), group C1-C2 (36,74 ± 15,13; 00,00 ± 0,00), group C1-T1 (36,74 ± 15,13; 00,00 ± 0,00), and group C1-T2 (36,74 ± 15,13; 15,00 ± 22,36), p=0,020. This study concluded that T1 group is giving the best effect on increasing life fetuses and decreasing embryo re-sorption percentage.