Kusumarwati, Arifah
Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

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Production and characterization of bacteriocin produced by lactic acid bacteria isolated from rusip Kusumarwati, Arifah; Indriati, Ninoek; Hermana, Irma
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): May 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.836 KB) | DOI: 10.15578/squalen.v8i1.77

Abstract

Research was conducted to produce and characterize bacteriocin produced by lactic acid bacteria (LAB) isolated from rusip, a traditional Bangkanese fermented fish product. Experiment was started by isolation of lactic acid bacteria from rusip, followed by screening to obtain the best isolate which has the highest bacterial inhibition activity. The selected isolate was then identified and used to produce crude bacteriocin. The crude bacteriocin was characterized through its stability in high temperature and proteolytic enzymes, inhibitory spectrum, pH sensitivity and effect of surfactants. The result showed that CN1.10a isolate which was identified as Lactococcus lactis subsp lactis has the highest bacterial inhibition activities; therefore it was selected to produce crude bacteriocin. The bacteriocin produced was heat stable, sensitive to proteolytic enzymes i.e. proteinase-K and papain but not to RNase. It inhibited Escherichia coli, Listeria monocytogenes and Lactobacillus plantarum. It stable at pH 2.0 to 6.0. Among surfactans used sodium dodecyl sulphate (SDS), lauryl sarcosine and EDTA were able to stimulate bacteriocin production, while the production were strongly inhibited by Tween 20, Tween 80, Triton X-100 and urea. Based on the above characteristic, the bacteriocin was suitable to be used as a preservative of food which has to be processed at high temperature.
Pembentukan Formaldehid Pada Ikan Kerapu (Epinephelus fuscoguffatus) Selama Penyimpanan Pada Suhu Kamar Riyanto, Rudi; Kusumarwati, Arifah; Dwiyitno, Dwiyitno
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 2 (2006): Desember 2006
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4628.318 KB) | DOI: 10.15578/jpbkp.v1i2.393

Abstract

Penelitian pembentukan formaldehid pada ikan kerapu macan (Epinephelus fuscoguttatus) selama penyimpanan pada suhu kamar telah dilakukan. Hasil penelitian menunjukkan bahwa formaldehid terbentuk pada proses pembusukan ikan dan lama penyimpanan ikan segar pada suhu kamar berpengaruh nyata terhadap pembentukan formaldehid secara alami pada ikan. Formaldehid yang terbentuk adalah 2,4‑3,0 ppm pada lama penyimpanan 20 jam, pada saat itu kadar air ikan mencapai 77%; TVB 515,7 mgN% dan nilai organoleptik 1,2.