Ratnaningtyas, Nuniek Ina
Fakultas Biologi | Universitas Jenderal Soedirman

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Karakterisasi Ganoderma spp. di Kabupaten Banyumas dan Uji Peran Basidiospora dalam Siklus Penyakit Busuk Batang Ratnaningtyas, Nuniek Ina; Samiyarsih, Siti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.233

Abstract

Ganoderma sp., one of fungi belonged to the family Ganodermataceae, order of Aphyllophorales, class of Basidiomycetes is very widely spread.  This fungus is a soil-borne, and has parasitic and saprophytic features which are interesting because of their two contradictory roles namely harmful and beneficial effects.  As a plant parasite, Ganoderma is able to cause root and stem rot of tropical perennial crop plantation and forest which cause serious losses.  The fungus is well known as white rot fungus which is able to cause a wood rotten by lignin destruction.  On the contrary, the fungus has some advantages such as medical potentials.  In this study, some collections and characterization of Ganoderma spp. found in Banyumas has been conducted.  The technique used for sampling Ganoderma spp. mushroom is a survey with Purposive Random Sampling method.  Samples were taken from nature in the area of Banyumas Regency and its natural substrate were noted. Characterization based on the macromorphology and micromorphology was done for grouping and identification.  Micromorphology observations were done by slicing and painting samples with plants microtechnique method.  The result showed that there were 43 Ganoderma spp isolates, distributed from low to high lands of 100 - 500 m above sea level.  The most fungi were found on the high lands, in Baturraden sub district, which included 16 isolates.  The interesting finding is the discovery of Ganoderma spp. which attacked flamboyant plant (Delonix regia) in West Purwokerto sub district.  Based on micromorphology observations, these fungi form a basidium and basidiospore inside and below as well as the surface of the fruit body.  Some species were also found having such phenomenon so it needs further research on the role of basidiospora in the cycle of stem rot disease, especially basidiospora role in the initiation of diseases infection.  As the soil infecting mushroom, study of the disease spreading is focused more on the role of the mycelium found in the remaining plants as infecting the material.  Basidiopore role in the spread of the disease is rarely studied, but actually basidiospore is a potential source of genetic variation.
KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST) Rohmah, Ross Nurul; Ratnaningtyas, Nuniek Ina; Asnani, Ari
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.1.22

Abstract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.
COMPATIBILITY OF INSECTICIDES WITH ENTOMOPATHOGENIC FUNGI Beauveria bassiana AND Metarhizium anisopliae Abidin, Azhar Faturohman; Ekowati, Nuraeni; Ratnaningtyas, Nuniek Ina
Scripta Biologica Vol 4, No 4 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2017.4.4.695

Abstract

Insecticide use has produced negative impact by affecting the non-target predatory organisms in nature, one of which is the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae. Interactions occur, however, between insecticides and the entomopathogens. The combination of insecticides at a low dose and an entomopathogenic fungus can work synergistically to increase pest insect mortality. This combination is particularly advantageous because it decreases the insecticide dose applied, reduces environmental contamination, and decreases pest resistance. The study purpose was to determine the compatible working insecticide dose and the entomopathogenic fungi B. basiana and M. anisopliae. The experimental design applied completely randomized design consisting of 15 treatments and four replicates. There were five types of insecticides with three different doses each (0.5 × Dose of Field (DF), 1 × DF, and 2 × DF), whereas the fungal isolates included B. basiana and M. anisopliae. The parameters observed were the germination percentage of conidia, the percentage of inhibition, and the number of conidia.ml-1. Data were analyzed using ANOVA (5% error rate). The mean values were analyzed by DMRT p < 0.05. Deltamethrin 0.5 x DF, and imidacloprid 0.5 x DF demonstrated the highest conidial germination in B. basiana and M. anisopli, respectively. Insecticides showing the highest vegetative growth on B. basiana and M. anisopliae at 7 DAI was imidacloprid 0.5 x DF, while at 14 DAI was imidacloprid 0.5 x DF and chlorphyrifos 2 x DF, respectively. The highest conidial production of the fungi was triggered by imidacloprid 0.5 x DF. Based on compatibility calculation, imidacloprid 0,5 x DF worked with B. basiana (BI: 67.77) and M. anisopliae (BI: 67.16).
DEKOLORISASI LIMBAH BATIK MENGGUNAKAN LIMBAH MEDIUM TANAM Pleurotus ostreatus PADA WAKTU INKUBASI YANG BERBEDA Wulandari, Fitriya Yuni; Ratnaningtyas, Nuniek Ina; Dewi, Ratna Stia
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.1.29

Abstract

The batik waste industry is one source of water pollution. It must undergo treatment before discharge into the environment. Batik waste treatment technologies can be done biologically, chemically, physically, or combination of three processes. This research applied a combination of biology and physic technologies, namely used spent mushroom of Pleurotus ostreatus as the decolorization agent. Mycelium of P. ostreatus was used as biological agent, and cellulose from spent mushroom was used as physical agent. The aims of this research were to determine the effect of spent mushroom with variation incubation time on batik waste decolorization, and to know the optimum incubation time with highest decolorization batik waste percentage using spent mushroom of P. ostreatus. This research used experimental method with completely randomized design (CRD). The data obtained were statistically analyzed using Varian Test (ANOVA) on confidence level of 95% and 99% and continued with Honest Significant Difference Test (BNJ) with 95% confidence level. The result showed that variation incubation time treatment of batik waste decolorization used spent mushroom of P. ostreatus provide a highly signification effect on batik waste decolorization. The optimum incubation time was 60 hour (LW5), with decolorization percentage was 85,64%.
PENGGUNAAN BEBERAPA MEDIUM SEMISINTETIK UNTUK PRODUKSI MISELIUM JAMUR MAITAKE (Grifola frondosa (Dickson: Fr.) S. F. Gray) ISOLAT CIANJUR DAN EKSTRAK KASARNYA Maharani, Maria Mardhitama; Ratnaningtyas, Nuniek Ina; Priyanto, Slamet
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.1.20

Abstract

Research on the use of some semisynthetic medium for the production of fungal mycelium Maitake (Grifola frondosa (dickson: Fr.) SF Gray) isolates Cianjur and crude extract was performed with an incubation period of 30 days. This study aimed to determine the ability of green bean, cowpea and maize as an alternative material of semisynthetic medium for manufacturing G. frondosa’s mycelium and and to know the production of G. frondosa’s mycelium and the highest crude extract. This study was experimental study with a completely randomized design consisted of 4 treatments : Yeast Potato Dextrose Broth (PDYB) medium, Green bean Yeast Dextrose Broth (GbDYB) medium, Cowpea Yeast Dextrose Broth (CpDYB) medium and Corn Yeast Dextrose Broth (CDYB) medium. The highest average dry weight of mycelium (1,584 g/100ml) was GbDYB medium. The lowest average dry weight of the mycelium (g/100ml 0.244) was PDYB medium. The weight of the crude extract of mycelium in each treatment was lower than the dry weight. The highest weight of the crude extract was obtained from the GbDYB medium treatment (1,22 g) and the lowest was obtained from PDYB medium (0,113 g). Anova test results of different treatment was very significant, meaning that the use of extract of green bean, cowpea and maize greatly affected the growth of G.frondosa’s mycelium. The LSD test between treatment of PDYB medium and CDYB medium was not significant, meaning that the increase of mycelium’s growth  on PDYB medium had no different with the CDYB medium.
BIODEGRADATION OF DIESEL OIL BY YEAST ISOLATED FROM MANGROVE’S RHIZOSPHERE Syah, Ryan Firman; Irianto, Agus; Ratnaningtyas, Nuniek Ina
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.2.814

Abstract

Diesel oil-degrading yeast strains isolated from mangrove rhizosphere at Tritih Kulon, Cilacap had been screened with SMSS medium. Four culturable yeast were isolated. Qualitative test was conducted by culturing and incubating the yeasts for one month in a medium added with 1mL diesel oil. By measuring the reduction of diesel oil, two best yeasts were selected. The quantitative test, GC-MS analysis, was conducted to determine the detailed degradation process of diesel oil. Candida lusitaniae and Cryptococcus laurentii performed the degradative ability. Three highest percent area of hydrocarbon compounds were compared for assessment. The results showed that C. lusitanie had better degradative capability than C. laurentii, in which hexadecane and methyl hexadecanoate decreased by 90–95%, and 9-octadecenoic acid, methyl ester declined by 30–40%. The increasing pH medium during incubation suggested that fermentation process occurred.
PRODUKSI MISELIUM Grifola frondosa (Dickson: Fries) Gray ISOLAT CIANJUR DAN BOBOT EKSTRAKNYA PADA MEDIUM MYPB DENGAN PENAMBAHAN BIJI BUNGA MATAHARI Helianthus annuus L. Maulida, Rizki; Ratnaningtyas, Nuniek Ina; Priyanto, Slamet
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.1.21

Abstract

Grifola frondosa or maitake, not only can be used as a food ingredient but also as medicine. Other than nutrients, fruit body and mycelium of G. frondosa also contains bioactive compounds, such as terpenoids, alkaloids, flavonoids, and beta glucans extracellular polysaccharides (β-1,3 glucans and β-1,6 glucans). Extracellular polysaccharide harvested more often in mycelium form which is cultivated in liquid medium. Liquid medium which is commonly used for the growth of mycelium is MYPB (Malt Yeast Peptone Broth). Mycelium production on MYPB as a medium can be increased by adding additional ingredients, one of which is sunflower (Helianthus annuus L.). The treatment was the addition of sunflower seeds on medium MYPB. The main parameters were mycelium’s dry weight and the weight of raw extract of G. frondosa. Supporting parameters were final pH medium, extracellular polysaccharides (qualitatively), terpenoid, alkaloid, and flavonoid compounds in the raw extract of G. frondosa. The addition of 250 g/l sunflower seeds in the medium MYPB was the optimum treatment that can produce 1,379 g/100ml of mycelium and 0,299 g/100ml G. frondosa extracts.