Rahaju, Sri Hartin
Research Center for Biology-Indonesian Institute of Sciences

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KANDUNGAN SELENIUM DALAM HERBA TERSELEKSI DARI DAERAH VULKANIS DAN AKTIVITAS GLUTATION PEROKSIDASE SERTA PENGARUHNYATERHADAPPENYUSUTAN SELMODEL Saccharomyces cerevisiae JB3505

BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

An exploration of selenium containing herbs was carried out in the Kerinci -Sumatera, Toraja highland-Sulawesi and Rinjani-Lombok. The herbs were sampled according to their morfofisiological characters and local etnopharmalogical information.The analitical parameters were the selenium and selenomethionine content as measured by AAS and GC respectively, gluthathione peroxidase as measured biochemically dan cell model shrinkage observation to reveal the selenium containing extract effect on celluler development. The result indicates the diversity of both content and functional selenium compounds in the selected herbs.The relatively high selenium content herbs such as A Ilium sativuin 1NHR had hingher gluthathione peroxidase and hence its antioxidant activity.However the relatively lower selenium content of Physalis angulata 33NHR was able to induce more cell model shrinkage. The phenomenon of relation among selenium based selenoamino acid, antioxidant and cell shrinkage potential need to be futher studied on these selected herbs.

ISOLASI BAKTERIOFAG Salmonella spp. dari BIOFILM pada SISTEM AIR MINUM ISI ULANG

Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The public demands for the refill drinking water increases causing the development of refill drinking water industries. However, the problems occurred is no standard method to process drinking water with sterile and lack of government oversight. These cases give rise to sanitation which is the formation of bacteria pathogen forming biofilms in refill drinking water system. One of the bacteria pathogens is Salmonella. Salmonella in refill drinking water can cause diarrhea, because it can produce cytotoxin and enterotoxin. Bacteriophages are viruses that infect bacteria. This study aims to find natural isolates of bacteriophage from biofilm samples to infect Salmonella spp. in refill drinking water system. The isolates obtained is then characterized by biochemical test including Gram stain, a test Kligler Iron Agar (KIA) and api assay 20 E. The positive Salmonella spp. isolates are in the second dilution refill drinking water depot. The isolation of bacteriophage from biofilm is conducted with bacteriophages amplification and bacteriophage filtrate. The Infection test is performed by using Salmonella enterica, Salmonella 7A1 from Teluk Ambon and Salmonella spp. from refill drinking water depot. Platting is performed on serial dilutions of 10-2 to 10-10phage dilution. Positive result is characterized by the formation of plaque which is in source water samples, water product and drinking water depot. The number of plaques formed is calculated by Plaque Forming Units (PFU/mL) to determine quantification or calculation phages.