Alimuddin, ,
Department of Aquaculture

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Digestive enzymes activities in Oreochromis niloticus fed diet supplemented with recombinant growth hormone

Jurnal Akuakultur Indonesia Vol 15, No 1 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT The specific activity of the digestive enzymes, namely: pepsin, amylase, lipase, trypsin, and chymotrypsin were studied in Nile tilapia Oreochromis niloticus fed diet supplemented with recombinant Ephinephelus lanceolatus growth hormone (rElGH). The results showed that fish treated with rE1GH showed lower lipase and chymotrypsin specific activities (P>0.05), while the trypsin/chymotrypsin specific activity (T/C ratio) was found higher compared to control fish. Moreover, higher protein digestibility, higher protein retention and a lower ammonia excretion rate were measured for rE1GH treated fish. Oral rElGH administration enhanced Nile tilapia growth up to 20.04%, without affecting survival. This study suggested that rapid growth performance induced by rElGH was linked with T/C ratio rather than the specific activity of other digestive enzymes. Keywords: recombinant growth hormone, digestive enzyme, digestibility, Oreochromis niloticus  ABSTRAK Aktivitas spesifik enzim pencernaan pepsin, amilase, lipase, tripsin, dan kemotripsin diamati pada ikan nila Oreochromis niloticus yang diberi pakan mengandung hormon pertumbuhan rekombinan ikan kerapu kertang Ephinephelus lanceolatus (rElGH)). Hasil menunjukkan bahwa ikan uji pada perlakuan rElGH memiliki aktivitas spesifik enzim lipase dan kemotripsin yang lebih rendah, sedangkan rasio tripsin/kemotripsin (rasio T/C) yang lebih tinggi dibandingan ikan kontrol. Kecernaan protein dan retensi protein bernilai lebih tinggi sementara laju ekskresi amonia bernilai lebih rendah pada ikan perlakuan. Pemberian rElGH secara oral mampu mempercepat laju pertumbuhan ikan nila hingga 20,04% tanpa memengaruhi kelangsungan hidup. Berdasarkan penelitian ini, dapat disimpulkan bahwa laju pertumbuhan cepat yang diinduksi oleh rElGH berhubungan dengan rasio T/C dibandingkan dengan aktivitas spesifik enzim pencernaan lain. Kata kunci: hormon pertumbuhan rekombinan, enzim pencernaan, kecernaan, Oreochromis niloticus 

Sexual dimorphism related to growth in climbing perch Anabas testudineus

Jurnal Akuakultur Indonesia Vol 15, No 1 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

 ABSTRCT The phenomenon that often occurs in climbing perch culture (Anabas testudineus, Bloch) is a significant size difference between individuals of male and female those lead to high variation in culture yields. In addition to genetic factors, the difference possibly reflects the sexual dimorphism in this fish; female grows faster than male. This research was conducted to examine sexual dimorphism related to growth quantitatively. Sixty individuals of 45-days old juvenile derived from mating of five pairs broods were randomly picked up and reared individually in 20×20×100 cm3 net cages for 135 days. Fish were fed on commercial feed (30% protein) three times a day at 10% feeding rate. Body weight and body length were individually recorded every 15 days, sex was determined at the end of the experiment by surgering the fish and gonad was weighed to calculate gonadosomatic index (GSI). The results showed that growth and specific growth rate of female were 1.17 and 1.48 fold respectively higher than male. This result indicates that female monosex culture of climbing perch is highly promising. Keyword: dimorphism, growth, climbing perch, Anabas testudineus  ABSTRAK Salah satu fenomena yang sering dijumpai dalam budidaya ikan papuyu (Anabas testudineus, Bloch) adalah perbedaan ukuran yang mencolok antara individu jantan dan betina yang menyebabkan hasil panen ikan sangat bervariasi. Selain faktor genetik, perbedaan ini diduga disebabkan oleh adanya dimorfisme seksual terkait pertumbuhan. Penelitian ini dilakukan untuk menguji dimorfisme seksual terkait dengan pertumbuhan ikan papuyu secara kuantitatif. Benih ikan umur 45 hari hasil pemijahan lima pasang induk diambil secara acak sebanyak 60 ekor dan dipelihara selama 135 hari secara individu di dalam 60 unit hapa (20×20×100 cm3). Ikan diberi pakan komersial (protein 30%) tiga kali sehari sebanyak 10% bobot tubuh. Bobot dan panjang semua ikan dicatat setiap 15 hari sekali, jenis kelamin ditentukan di akhir penelitian dengan cara membedah, mengamati gonad, dan menimbangnya untuk menentukan indeks gonadosomatik (IGS). Hasil penelitian membuktikan adanya dimorfisme seksual terkait pertumbuhan pada ikan papuyu; pertumbuhan bobot mutlak dan laju pertumbuhan bobot harian ikan betina masing-masing 1,48 kali dan 1,17 kali lebih tinggi dibandingkan dengan ikan jantan. Hasil penelitian ini mengindikasikan bahwa pengembangan budidaya ikan papuyu monoseks betina prospektif untuk dilakukan. Kata kunci: dimorfisme, pertumbuhan, papuyu, Anabas testudineus 

Growth and survival of giant gourami juvenile immersed in different doses of recombinant growth hormone

Jurnal Akuakultur Indonesia Vol 10, No 2 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRAKPenelitian ini dilakukan untuk meningkatkan pertumbuhan benih ikan gurami (Osphronemus goramy) melalui imersi dengan hormon pertumbuhan rekombinan ikan gurami (r-OgGH). Imersi dengan larutan mengandung badan inklusi dilakukan dengan dosis r-OgGH berbeda, yakni 0 mg/L (kontrol), 10 mg/L, 20 mg/L, dan 30 mg/L, sekali seminggu selama tiga minggu. Ikan dipelihara selama lima minggu dalam akuarium dengan kepadatan 5 ekor/L, dan ikan diberi pakan naupli Artemia dan cacing sutera dua kali sehari secara ad libitum. Hasil penelitian menunjukkan bahwa rerata bobot tubuh ikan perlakuan 30 mg/L (0,34 g) adalah sekitar 75% lebih tinggi (p<0,05) daripada perlakuan 10 mg/L (0,24 g) dan kontrol (0,19 g). Kelangsungan hidup tertinggi diperoleh pada perlakuan 30 mg/L (100%), diikuti oleh perlakuan 20 mg/L (96%), perlakuan 10 mg/L (94%), dan kontrol (94%). Dengan demikian, imersi menggunakan hormon pertumbuhan rekombinan dapat digunakan untuk meningkatkan pertumbuhan dan kelangsungan hidup benih ikan gurami.Kata kunci: protein rekombinan, hormon pertumbuhan, imersi, Osphronemus goramy ABSTRACTThis study was aimed at enhancing the growth of giant gourami Osphronemus goramy fry by immersion with recombinant giant gourami growth hormone (r-OgGH). Immersion with solution containing inclusion bodies with different doses of r-OgGH, 0 mg/L (control), 10 mg/L, 20 mg/L, and 30 mg/L was performed on weekly basis for the first three weeks of experimental period. Fish were reared for five weeks in glass aquaria at a stocking density of 7 fish/L, and fed with Artemia nauplii and blood worm twice a day ad libitum. The results showed that the average body weight of 30 mg/L r-OgGH treated fish (0.34 g) was 75% higher (p<0.05) than those of 10 mg/L (0.24 g) and control (0.19 g). The highest survival was obtained at 30 mg/L r-OgGH immersed fish (100%), followed by 20 mg/L (96%) and 10 mg/L treated fish (94%), and control (94%). The results indicate that immersion with recombinant growth hormone could be applied to enhance the growth and survival of giant gourami juvenile.Keywords: recombinant protein, growth hormone, immersion, Osphronemus goramy