Lotulung, Puspa Dewi
Research Center for Chemistry – Indonesian Institute of Sciences, Kawasan PUSPIPTEK, Serpong – 15314, South Tangerang, Banten, Indonesia

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The Synthesis of Cinchonine Tiglat Ester Compound and Cytotoxic Test Against MCF-7 Breast Cancer Cell Khanifudin, Ahmad; Primahana, Gian; Prima, Sylvia Rizky; Lotulung, Puspa Dewi; Hanafi, Muhammad
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 19, No 2 (2017)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.773 KB) | DOI: 10.14203/jkti.v19i2.354

Abstract

Cinchonine is a type of cinchona alkaloid compound commonly found and/or isolated from Cinchona sp. plant. It is commonly used to treat malaria, and can potentially be used against cancer cells. In this particular study, cinchonine ester derivatives were extracted through esterification processs. Synthesized esther is aimed to gain higher lipophilicity of cinchonine so that makes it easier to pass through cell membrane. Esterification was done using DCC activator as well as DMAP catalyst with tiglic acid used to create cinchonine tiglat. Subsequent cinchonine tiglat was obtained in the form of oil with 25,28% yield. The compound obtained from the synthesis was the analyzed using LC-ESI-MS and 1H-NMR spectroscopy instrumentation. Results show that the target compound has been successfully synthesized. Its cytotoxic ability against MCF-7 breast cancer cells was tested using the Alamar Blue method. Results concluded that cinchonine tiglat ester compound has a viable cytotoxic activity with IC50 value of 1.22 ppm.
Synthesis and cytotoxicity assay using Brine Shrimp Lethality Test of Cinchonidine Isobutyrate Ester Mario, Mario; Lotulung, Puspa Dewi; Primahana, Gian; Prima, Sylvia Rizky; Hanafi, Muhammad
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 19, No 1 (2017)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.382 KB) | DOI: 10.14203/jkti.v19i1.328

Abstract

This research was aimed to synthesize cinchonidine isobutyrate ester and conduct a preliminary assay for anticancer agent using cytotoxicity assay to Artemia salina Leach larva, or also known as brine shrimp lethality test (BSLT). Cinchonidine, a compound that has quinoline rings and quiniclidine ring, is a quinine analogue and stereoisomer of cinchonine. Cinchonidine is predicted to have anticancer activity. Synthesized ester was aimed to gain higher lipophilicity. Higher lipophilicity makes it easier for the compund to pass through cell membrane. The esterification process used DMAP as a catalyst, DCC as an activator, and isobutyric acid as a carboxilyc acid. Isobutyric acid is a type of short chained fatty acid that usually acts as an anticancer prodrugs. The product is identified by ESI-MS, FT-IR, 1H-NMR, dan 13C-NMR. Ester cinchonidine isobutyrate is gelatinous and colourless with yield of 21,77%. BSLT result showed that cinchonidine isobutyrate ester had LC50 value of 75.16 ppm which was more toxic than cinchonidine that had LC50 value of 99.2 ppm. It was proved that higher lipophilicity could increase pharmacology activity
Preparation of Alkyl Halide as Intermediate Compound in Synthesis Cationic Surfactant Alkyl Trimethyl Ammonium Chloride Sari, Asdani Muatika; Suryani, Ani; Lotulung, Puspa Dewi; Tursiloadi, Silvester
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 19, No 1 (2017)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.868 KB) | DOI: 10.14203/jkti.v19i1.327

Abstract

Cationic surfactant alkyl trimethyl ammonium chloride was synthesized by quartenerisation of tertiary amines. Materials used in quartenerization are tertiary amine and alkyl halide. Alkyl halide is a hydrocarbon derivative in which one or more hydrogen is replaced with halogen. In this research, thionyl chloride is used as a reactant . Thionyl chloride (SOCl2) is often used because it is easier to make, the yield is greater and byproducts are volatile. Alkyl halide is synthesized from the reaction of hexadecyl alcohol with thionyl chloride (SOCl2) at a temperature of 80 C for 24 hours in a reflux reactor. This study managed to get hexadecyl chloride from the reaction hexadecyl alcohol and thionyl chloride. It can be seen from the results of FTIR, LC/MS and GC/MS
Formulation of emulsion of soybean (Glycinia soyae L.) tempeh and fermented spinach (Amaranthus sp.) using combination of gelatin and CMC as thickener Susilowati, Agustine; Aspiyanto, Aspiyanto; Lotulung, Puspa Dewi; Maryati, Yati
Indonesian Journal of Pharmaceutical Science and Technology Vol 6, No 3 (In Press)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v6i3.20204

Abstract

Mixture of soybean (Glycinia soyae L.) tempeh paste and spinach (Amaranthus sp.) fermented by Kombucha culture produces potential source of natural folic acid. The experiment activity aims to find out the best ratio of gelatin and CMC as thickener in emulsification to prepare supplement of natural folic acid. Emulsification process was conducted by adding gelatin and CMC at ratio of 0 : 0, 0.1 : 2, 0.2 : 4, 0.3 : 6, 0.4 : 8, and 0.5 : 10 (g/g) in mixture of fermented spinach concentration of 2.5, 5.0, 7.5, and 10.0% (w/w in soybean tempeh) and soybean tempeh. The best drying treatment was achieved at 50°C for 24 h. Result of experiment activity showed that based on the best folic acid, the optimum treatment to yield supplement paste was achieved at combination of gelatin-CMC ratio of 0.4 : 8 (g/100 g in mixture paste) and fermented spinach 2.5% (b/b in soybeans tempeh). Identification on folic acid in paste and supplement powder with mass spectra had been identified 6 folic acid monomer dominated by folic acid monomer with molecular weight (M+1) 442.87 and 442.66 Dalton (Da.) with relative intensity 100%. Paste and powder of supplement displayed distribution of particle size 1,745.1 and 984.1 nm with particle indexes of 1.124 and 1.121.Keywords : folic acid , gelatin, emulsification, tempeh, spinach, ratio.