Safika, Safika
Universitas Syiah Kuala

Published : 4 Documents
Articles

Found 4 Documents
Search

9. Total colony of cellulolitic bacteria in the rumen of aceh cattle Safika, Safika; Matondang, Siti Wardianah; Darmawi, Darmawi; Abral, Mahdi; Erina, Erina; Jalaluddin, M
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.784 KB) | DOI: 10.21157/j.med.vet..v11i1.3411

Abstract

The aim of this study was to count the total sum of cellulolitic bacteria colony in  aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml
2. Antibacterial Test of Red Dragon Fruit Extract Peel (Hylocereus polyrhizus) Against Bacteria Salmonella pullorum Maulana, Ichsan; Harris, Abdul; Fakhrurrazi, Fakhrurrazi; Dewi, Maryulia; Safika, Safika; Erina, Erina; Jalaluddin, Muhammad
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.06 KB) | DOI: 10.21157/j.med.vet..v12i1.4241

Abstract

The purpose of this study is to determine the effect of the red dragon fruit peels (Hylocereus polyrhizus) extract on the growth of Salmonella pullorum. This research was conducted at the Laboratory of Microbiology, Veterinary Faculty, University of Syiah Kuala, Banda Aceh. Red dragon fruit peels that used in this study is obtained from  ripe fruit and Salmonella pullorum cultures is  obtained from  Microbiology Laboratory of Veterinary Faculty Unsyiah. Salmonella pullorum culture added evenly on the surface of Mueller Hinton Agar, the number of the bacteria have been standarized using Mc Farland1 method. Discs soaked in the red dragon fruit peels extracts at different concentrations (20 mg/ml, 40 mg/mL, and 60 mg/ml). Ampicillin is used as positive controls and DMSO (dimethyl sulfoxide) was placed on the surface of Mueller Hinton Agar. The parameters in this study is the inhibition zone diameter. Data were analyzed descriptively. The results showed that the red dragon fruit peel extract is able to inhibit the growth of Salmonella  pullorum and the average of  inhibition diameter zone at a concentration 60 mg / ml is 9.6 mm, 40 mg / ml is 9.4 mm and 20 mg / ml is 9.3 mm
19. The Effect of AKBIS Probiotic Adding that Fermented in Fodder toward the Total of Aspergillus niger of Broiler’s Gastrointestinal Tract Nurrady, Cut Lathifa; Nurliana, Nurliana; Safika, Safika; Ferasyi, T. Reza; Ismail, Ismail; Muttaqien, Muttaqien
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.507 KB) | DOI: 10.21157/j.med.vet..v12i2.4051

Abstract

This study aimed to determine the total of A. niger found in the gastrointestinal tract of broilers after the addition of soybean dregs and fermented palm kernel meal by A.niger as probiotics (AKBIS Prob) in the fodder. This study used a complete randomized factorial design, which consists of two factors, namely factor AKBIS concentration and duration of AKBIS Prob. The treatment was divided into the group as a control P0, P1, P2 and P3 which consisted of five broilers, respectively given feed supplemented with AKBIS Prob 2, 4 and 6%. The provision of AKBIS Prob has done for 21 days and 35 days. On the 22nd and 36th day the contents of the caecum were taken. The total of A. niger was obtained using Total Plate Count (TPC) method. The data analysis used a factorial analysis of variance. The results showed that the concentration of 2, 4 and 6% did not affect (P> 0.05) the total of A. niger in the caecum of broilers, while the provision of AKBIS prob in fodder for 21 and 35 days had a significant effect (P <0.05) to the total A. niger. It can be concluded that the provision of the concentration of AKBIS Prob up to 6% in the fodder decreases the total of A. Niger. The provision of AKBIS Prob for 35 days can increase the total of A. niger in the digestive tract of broilers.
20. The Isolation of Salmonella sp. on quail eggs (cortunxi-cortunix japonica) that failed to hatch in Garot, Darul Imarah Subdistrric, Aceh Besar Suryandari, Latifa; Erina, Erina; Darniati, Darniati; Safika, Safika; Asmilia, Nuzul; Salim, M. Nur
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.432 KB) | DOI: 10.21157/j.med.vet..v12i2.4118

Abstract

The study aimed to isolate the Salmonella sp on quail eggs that failed to hatch in Garot village, Darul Imarah Subdistrict, Aceh Besar. A total of thirty quail eggs that failed to hatch were examined in microbiology laboratory of Veterinary Medicine Faculty, Syiah Kuala University. Modification of Carter method was used in this study to isolated samples. The eggs were opened for taking the embryo and then it was swabbed with sterile swabs.  The swab was put into Selenite Cystine Broth (SCB) and incubated at 37o C for 24 hours. The breed was then transferred onto Salmonella Shigella Agar (SSA). The growing Bacteria were observed for their morphology and Gram staining to make sure the bacteria belonged to the negative Gram. The result showed that 10 of the 30 of quail eggs that failed to hatch were positively infected with Salmonella sp. Based on this study, it can be concluded that the salmonella sp.can be isolated from quail eggs which were not hatched in the farm of Garot, Darul Imarah Subdistrict, Aceh Besar