Abinawanto, Abinawanto
Research Center for Biology-Indonesian Institute of Sciences

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VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis]

BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.

Peranan Dimetil Sulfoksida sebagai Krioprotektan dalam Mempertahankan Kualitas Spermatozoa Ikan Gurami, Osphronemus goramy Lacepede, 1801, Dua Puluh Empat Jam Pascakriopreservasi

Journal of Biota BIOTA Volume 16 Nomor 1 Tahun 2011
Publisher : Journal of Biota

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Abstract

Penelitian dilakukan dengan tujuan mengetahui pengaruh berbegai konsentrasi DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%) sebagai krioprotektan terhadap kualitas spermatozoa ikan gurami, Osphronemus goramy Lacepede, 1801, dua puluh empat jam pascakriopreservasi. Penelitian dilakukan di Balai Besar Pengembangan Budidaya Air Tawar (BBPBAT), Sukabumi. Semen dikoleksi dengan cara pengurutan (stripping) dan dievaluasi secara makroskopis (warna, pH, dan volume) dan mikroskopis (persentase motilitas, viabilitas, abnormalitas, dan konsentrasi spermatozoa) baik sebelum maupun sesudah kriopreservasi. Semen diencerkan dengan pengencer yang mengandung ekstender 189M dan krioprotektan DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%), disimpan pada cryotube 2 ml dan dikriopreservasi selama 24 jam. Peningkatan konsentrasi DMSO memengaruhi kualitas spermatozoa ikan gurami pascakriopresevasi. Konsentrasi DMSO yang terbaik berdasarkan hasil pengamatan adalah DMSO konsentrasi 13% (P > 0,05) dengan persentase motilitas sebesar 68,58%; abnormalitas sebesar 29%; dan viabilitas sebesar 63,5%.

The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing

Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

The spermatozoa quality of goramy two days after sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively.  Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for two days. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.Keywords: glycerol; Osphronemus goramy; spermatozoa motility, viability, and abnormality; sub-zero freezing