Suryaningrum, Lusi Herawati
Research Center for Biology-Indonesian Institute of Sciences

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PENGARUH PENAMBAHAN GLISEROL PADA PAKAN TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN NILA (Oreochromis niloticus) [Effect of Glycerol Addition into Fish Feed on the Growth and Survival Rate of Nile Tilapia (Oreochromis niloticus)] Suryaningrum, Lusi Herawati; Mulyasari, Mulyasari; Samsudin, Reza
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3201.575 KB) | DOI: 10.14203/beritabiologi.v16i2.2833

Abstract

The aim of this research was to determine the optimum percentage of glycerol addition into the diets of  Nile Tilapia (Oreochromis niloticus) on growth performance, feed convertion ratio, and survival rate. The research was conducted using 15 aquaria with the size of 60 x 50 x 45 cm3, each containing 25 O. niloticus with average initial weight of 4.63 ± 0.15 g. The experiment was conducted using a complete random design with five treatments and three replications. Isonitrogenous (31%) and isocaloric (17 MJ.kg-1) diets were provided for 60 days of rearing period. Five diets were formulated with glycerol content of 0% (G0); 5% (G5); 10% (G10); 15% (G15), and 20% (G20). Fish were fed twice daily with experimental diet at satiation level. The result showed that addition of 10% glycerol in the diet (G10) presented the highest values for weight gain (105.85 ± 0.40%), specific growth rate (3.44 ± 0.01%), protein retention (27.75 ± 0.02%), protein efficiency ratio (2.53 ± 0.15%), lipid retention (60.44 ± 0.03%), and feed convertion ratio (1.59 ± 0.01%) (P < 0.05). Survival rate was not affected by dietary containing glycerol (P > 0.05). Therefore, addition of 10% glycerol into the diet gave a significant increase on growth performance and feed convertion ratio without adverse effect on survival rate of Nile Tilapia.  
ISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTSISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTS Mulyasari, Muyasari; Sunarno, Mas Tri Djoko; Suryaningrum, Lusi Herawati
Indonesian Aquaculture Journal Vol 13, No 2 (2018): (December, 2018)
Publisher : Pusat Riset Perikanan, Kementerian Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.641 KB) | DOI: 10.15578/iaj.13.2.2018.83-93

Abstract

Protease is one of the potential enzymes used to improve the quality of local raw materials as fish feedstuff. The aim of this research was to find high protease activity-bacteria isolates in improving protein digestibility of fish feedstuff. Isolation was conducted on various growth substrates such as water and sediment of hot springs, mangrove sediment, and shrimp head waste. The parameters used in the selection and characterization of potential proteolytic bacteria were qualitative and quantitative tests of proteolytic activity. Microbes that have the highest enzyme activity were then selected and characterized based on their growth curve, production time of protease, and determination of optimum condition (temperature and pH) of protease activity. The result showed that there are only 18 isolates from 44 isolates positively had protease activity (qualitatively). Three isolates with the highest activity (RGL1.1, PL1.1, and PL1.2) qualitatively and quantitatively were then selected to be tested for their pathogenecity on tilapia. The results showed that only RGL1.1 was not pathogenic and therefore, potentially used as a protease producer candidate. The characterization of RGL1.1 isolates showed that the exponential phase of RGL1.1 was thirty-sixth hour in line with the optimum activity of enzyme production. The optimum pH and temperature of RGL1.1 isolate were 6 and 50°C, respectively. RGL1.1 isolate is a Gram-positive rod-shaped bacteria that has 99.53% of resemblance to Bacillus cereus.