Undap, Suzanne L.
Master Program of Aquatic Sciences

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Public perception on the application of eco-fishing port in Ocean Fishing Port of Bitung, North Sulawesi Zebblon, Passion Ch.; Undap, Suzanne L.; Lasut, Markus T.
AQUATIC SCIENCE & MANAGEMENT (Jurnal Ilmu dan Manajemen Perairan) Vol 4, No 1 (2016): April
Publisher : Master Program of Aquatic Sciences

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Abstract

Title (Bahasa Indonesia): Persepsi masyarakat terhadap penerapan eco-fishing port di Pelabuhan Perikanan Samudera (PPS) Bitung, Sulawesi Utara. This study was aimed to analyze public perception on the Eco Fishing Port, in Ocean Fishing Port of Bitung, North Sulawesi. It used interviews and questionnaires to all  stakeholders of the fisheries port. The analysis employed SWOT to study internal and external factors affecting the Eco Fishing Port management. Based on the SWOT analysis, the policy strategy of the Eco Fishing Port implementation in Bitung should apply many priority action plans, such as program continuity through government and private budget collaboration, possible extension of fisheries port area, appointment of professional manager of fish landing center, product diversification, waste utilization, environmentally friendly and renewable energy utilization, and blue economic concept-based stakeholder development approach. An integrated waste water treatment installation and reporting development of environmental management plan should also be done through implementation of ISO=14.001 management and certification  In addition, increased attention needs to focus on social and economic development, periodic environmental impact analysis, environmental hygiene, port facility restructure, better management of fish landing center space, and port ecology. Penelitian ini bertujuan untuk menganalisis persepsi masyarakat terhadap eco-fishing port (EFP) di Pelabuhan Perikanan Samudera (PPS) Bitung, Provinsi Sulawesi Utara. Metode penelitian menggunakan wawancara mendalam dan kuisioner kepada seluruh stakeholder pelabuhan perikanan ini. Analisis SWOT juga digunakan untuk mengawali kajian internal dan eksternal faktor yang mempengaruhi pengelolaan EFP. Hasil analisis menunjukkan, strategi kebijakan penerapan EFP di Kota Bitung, perlu disusun rencana tindak prioritas terkait dengan keberlanjutan program melalui kolaborasi pendanaan antara pemerintah dan konsorsium (swasta nasional/internasional), perluasan daerah PPS Bitung sesuai ketersedian lahan yang memungkinkan, penunjukan pengelola tempat pendaratan ikan (TPI) yang professional, diversifikasi produk, pemanfaatan limbah, pemanfaatan energi ramah lingkungan dan terbarukan, dan pendekatan pembinaan stakeholder dengan konsep blue economy. Pengembangan instalasi pengelolaan air limbah terpadu dan peningkatan pelaporan Rencana Kelola Lingkungan (RPL) juga perlu dilakukan melalui pelaksanaan manajemen dan sertifikasi ISO 14.001. Di samping itu, peningkatan perhatian perlu juga difokuskan pada pengembangan aspek sosial dan ekonomi, dampak lingkungan sesuai kondisi terkini, manajemen sanitasi lingkungan, restrukturisasi fasilitas pelabuhan perikanan, kualitas konstruksi dan tataruang tempat pendaratan ikan (TPI), dan ekologi pelabuhan.
Isolation and amplification of 16S rRNA gen of Associated Microbial isolates in Red Algae Kappaphycus alvarezii from Belang, Southeast Minahasa Regency, North Sulawesi Wantania, Letha L.; Wullur, Stenly; Ginting, Elvi L.; Mantiri, Desy M. H.; Undap, Suzanne L.; Sumilat, Deiske A.; Gerung, Grevo S.
JURNAL ILMIAH PLATAX Vol 7, No 1 (2019): EDISI JANUARI-JUNI 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.22808

Abstract

This study aims to obtain isolates and amplify the associative bacterial 16SrRNA gene in K. alvarezii algae. The K. alvarezii algae was collected from seaweed cultivation area in Belang, Southeast Minahasa, North Sulawesi. Associative bacteria were sampled from K. alvarezii algae, grown in Nutrient Agar and separated based on their morphological characteristics.  Each isolates were extracted their DNA genome and.the16S rRNA gene of each isolate was amplified using PCR.  Eight associative bacterial from K. alvarezii algae were successfully isolated based on morphological characteristics which were dominated by round shape, smooth edges, convex elevation, and white color of the isolates. The results of genomic DNA extraction from each of these isolates were successfully used as templates to amplify the 16s rRNA gene.Key word: Bacteria, Kappaphycus alvarezii, Taxsonomy, 16S rRNAABSTRAKPenelitian ini bertujuan untuk mendapatkan isolat dan mengamplifikasi gen 16SrRNA bakteri asosiatif pada alga K. alvarezii. Metode penelitian diawali dengan pengambilan sampel alga K. alvarezii di lokasi pembudidayaan rumput laut di desa Belang, Minahasa Tenggara, Sulawesi Utara. Selanjutnya, bakteri asosiatif pada alga K. alvarezii tersebut ditumbuhkan dalam media Nutrient Agar, isolat yang tumbuh kemudian dipisahkan berdasarkan karakteristik morfologinya. dan gen 16S rRNA masing-masing isolat diamplifikasi menggunakn PCR.  Delapan isolat bakteri asosiatif pada alga K. alvarezii berhasil diisolasi dengan karakteristik morfologi berbeda yang didominasi dengan bentuk round, tepian smooth, elevasi convex, dan warna putih. Hasil ekstraksi DNA genom dari masing-masing isolat tersebut berhasil digunakan sebagai template untuk mengamplifikasi gen 16s rRNA.Kata Kunci: Bakteri, Kappaphycus alvarezii, Taksonomi, 16S rRNA
Application of probiotic bacteria isolated from catfish (Clarias batrachus) intestine to enhance growth performance and resistance of carp (Cyprinus carpio) against Aeromonas hydrophila Saragih, Febi S.D.; Manoppo, Henky; Undap, Suzanne L.; Ngangi, Edwin L. A.; Longdong, Sammy N. J.; Kusen, Diane J.
JURNAL ILMIAH PLATAX Vol 7, No 2 (2019): EDISI JULI - DESEMBER 2019
Publisher : Sam Ratulangi University

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Abstract

The objective of research was to isolate probiotic bacteria from catfish intestine and to examine its effects on the growth and resistance of carp against Aeromonas hydrophila.  The probiotic isolated from intestine was grown on MRS agar.  Fish used in this research was juvenile of carp with an average weight of 5.5 g obtained from Freshwater Aquaculture Board at Tatelu Village, North Sulawesi Province.  Before running the experiment, the juveniles were adapted for one weeks in 15 glass aquaria at a density of 15 individuals each.  After acclimatization, the fish were fed diet supplemented with probiotic at different concentrations namely 1x109 , 1x108 , 1x107,  1x106 cfu/mL for three weeks. The fish were fed two times a dayat 08.00 am and 17.00 pm with a dose of 5% of body weight per day. At the end of feeding, the fish were challenged with A. hydrophila.  Data collected included average growth rate, absolute growth, feed efficiency, and food conversion ratio.  Research results found that the addition of probiotic into feed was  able to increase growth and feed efficiency and reduce food conversion ratio as well (p<0.01). The best growth, feed efficiency and food conversion ratio were obtained in fish fed diet added with probiotic bacteria at 1x108 cfu/mL.  The highest survival rate was also observed in fish probiotic diet containing 1x108 cfu/mL.  As conclusion, probiotic bacteria isolated from catfish intestine was potential to improve growth, feed efficiency, reduce food conversion ratio and increase resistance of fish against bacterial pathogen.Keywords: probiotic, carp, Aeromonas hydrophila, feed efficiency, food conversion ratioABSTRAKPenelitian ini bertujuan untuk mengisolasi bakteri probiotik dari usus ikan lele dan menguji pengaruh pemberian probiotik dari usus ikan lele terhadap pertumbuhan dan resistensi ikan mas terhadap infeksi Aeromonas hydrophilla. Sumber probiotik diisolasi dari usus ikan lele menggunakan media MRS (de Man Rogosa Sharpe) dan bakteri Aeromonas hydrophilla ditumbuhkan pada media TSA (Tryptic Soy Agar). ikan uji diambil dari Balai Budidaya Air Tawar Tatelu , Provinsi Sulawesi Utara . ikan diaklimatisasi selama seminggu dalam 15 akuarium dengan kepadatan 15 ekor/akuarium dan berat awal rata-rata 5,5 g. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan bakteri probiotik sebagai perlakuan dengan konsentrasi berbeda yaitu 1x109 , 1x108 , 1x107,  1x106 cfu/mL selama empat minggu sebanyak 5% /berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 08.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan maka diuji tantang dengan bakteri Aeromonas hydrophilla.  Data yang dikumpulkan terdiri dari laju pertumbuhan harian, pertumbuhan mutlak, efesiensi pakan, konversi pakan dan uji tantang terhadap bakteri patogen. Hasil penelitian mendapatkan bahwa penambahan bakteri probiotik dalam pakan mampu meningkatkan pertumbuhan, efesiensi pakan dan menurunkan konversi pakan (p<0.01). Pertumbuhan, efesiensi pakan dan konversi pakan yang terbaik dicapai pada ikan yang diberi pakan dengan penambahan bakteri probiotik 1x108 cfu/mL.  Kelangsungan hidup ikan mas yang diuji tantang dengan bakteri patogen yang paling tinggi dicapai pada ikan yang diberi pakan 1x108 cfu/mL. Sebagai kesimpulan bahwa bakteri probiotik yang diisolasi dari usus ikan lele berpotensi meningkatkan pertumbuhan, efesiensi pakan, menurunkan konversi pakan serta meningkatkan sistem kekebalan tubuh pada ikan mas.Kata Kunci : Probiotik, Ikan Mas, Aeromonas hydrophilla, Pertumbuhan, Efesiensi Pakan, Konversi Pakan
Application of probiotic bacteria isolated from catfish (Clarias batrachus) intestine to enhance growth performance and resistance of carp (Cyprinus carpio) against Aeromonas hydrophila Saragih, Febi S.D.; Manoppo, Henky; Undap, Suzanne L.; Ngangi, Edwin L. A.; Longdong, Sammy N. J.; Kusen, Diane J.
JURNAL ILMIAH PLATAX Vol 7, No 2 (2019): EDISI JULI - DESEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23724

Abstract

The objective of research was to isolate probiotic bacteria from catfish intestine and to examine its effects on the growth and resistance of carp against Aeromonas hydrophila.  The probiotic isolated from intestine was grown on MRS agar.  Fish used in this research was juvenile of carp with an average weight of 5.5 g obtained from Freshwater Aquaculture Board at Tatelu Village, North Sulawesi Province.  Before running the experiment, the juveniles were adapted for one weeks in 15 glass aquaria at a density of 15 individuals each.  After acclimatization, the fish were fed diet supplemented with probiotic at different concentrations namely 1x109 , 1x108 , 1x107,  1x106 cfu/mL for three weeks. The fish were fed two times a dayat 08.00 am and 17.00 pm with a dose of 5% of body weight per day. At the end of feeding, the fish were challenged with A. hydrophila.  Data collected included average growth rate, absolute growth, feed efficiency, and food conversion ratio.  Research results found that the addition of probiotic into feed was  able to increase growth and feed efficiency and reduce food conversion ratio as well (p<0.01). The best growth, feed efficiency and food conversion ratio were obtained in fish fed diet added with probiotic bacteria at 1x108 cfu/mL.  The highest survival rate was also observed in fish probiotic diet containing 1x108 cfu/mL.  As conclusion, probiotic bacteria isolated from catfish intestine was potential to improve growth, feed efficiency, reduce food conversion ratio and increase resistance of fish against bacterial pathogen.Keywords: probiotic, carp, Aeromonas hydrophila, feed efficiency, food conversion ratioABSTRAKPenelitian ini bertujuan untuk mengisolasi bakteri probiotik dari usus ikan lele dan menguji pengaruh pemberian probiotik dari usus ikan lele terhadap pertumbuhan dan resistensi ikan mas terhadap infeksi Aeromonas hydrophilla. Sumber probiotik diisolasi dari usus ikan lele menggunakan media MRS (de Man Rogosa Sharpe) dan bakteri Aeromonas hydrophilla ditumbuhkan pada media TSA (Tryptic Soy Agar). ikan uji diambil dari Balai Budidaya Air Tawar Tatelu , Provinsi Sulawesi Utara . ikan diaklimatisasi selama seminggu dalam 15 akuarium dengan kepadatan 15 ekor/akuarium dan berat awal rata-rata 5,5 g. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan bakteri probiotik sebagai perlakuan dengan konsentrasi berbeda yaitu 1x109 , 1x108 , 1x107,  1x106 cfu/mL selama empat minggu sebanyak 5% /berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 08.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan maka diuji tantang dengan bakteri Aeromonas hydrophilla.  Data yang dikumpulkan terdiri dari laju pertumbuhan harian, pertumbuhan mutlak, efesiensi pakan, konversi pakan dan uji tantang terhadap bakteri patogen. Hasil penelitian mendapatkan bahwa penambahan bakteri probiotik dalam pakan mampu meningkatkan pertumbuhan, efesiensi pakan dan menurunkan konversi pakan (p<0.01). Pertumbuhan, efesiensi pakan dan konversi pakan yang terbaik dicapai pada ikan yang diberi pakan dengan penambahan bakteri probiotik 1x108 cfu/mL.  Kelangsungan hidup ikan mas yang diuji tantang dengan bakteri patogen yang paling tinggi dicapai pada ikan yang diberi pakan 1x108 cfu/mL. Sebagai kesimpulan bahwa bakteri probiotik yang diisolasi dari usus ikan lele berpotensi meningkatkan pertumbuhan, efesiensi pakan, menurunkan konversi pakan serta meningkatkan sistem kekebalan tubuh pada ikan mas.Kata Kunci : Probiotik, Ikan Mas, Aeromonas hydrophilla, Pertumbuhan, Efesiensi Pakan, Konversi Pakan