Indriawati, Indriawati
Research Center for Biology-Indonesian Institute of Sciences

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Genetic Diversity and Relationship among Bali Cattle from Several Locations in Indonesia Based on ETH10 Microsatellite Marker Margawati, Endang Tri; Volkandari, Slamet Diah; Indriawati, Indriawati; Ridwan, Muhamad
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 4 (2018): DECEMBER 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.619 KB) | DOI: 10.14334/jitv.v23i4.1915

Abstract

Bali cattle is one of local beef cattle in Indonesia, up to present its performance indicated an inbreeding occurrence. This study was aimed to analyze the genetic diversity and relationship among Bali cattle from several locations in Indonesia based on ETH10 microsatellite marker. Ninety-four (94) DNA samples (89 Bali cattle; 5 Banteng) were analyzed. The Bali cattle samples were from 6 locations in Indonesia (15 Pulukan; 15 Nusa Penida; 14 Bima West Nusa Tenggara/WNT; 10 Mataram, WNT; 20 Riau; 15 South Borneo). DNA Banteng samples were collected from Prigen Malang of East Java. Microsatellite marker of ETH10 labelled HEX was used for amplification. Alleles were analyzed by using Cervus 3.0.7 and GenAlex 6.5. Result showed that there were five (5) alleles found in ETH10 marker i.e., 209; 213; 215; 217; and 219 bp. Average of observed (Ho) and expected (He) heterozygosity were 0.46±0.05 and 0.60±0.03, respectively. Five (5) out of 6 locations were in breeding occurrence except Bali cattle from Mataram was not inbreeding. The longest genetic relationship was between Bali cattle from Mataram and Riau whereas the closest distance was Bali cattle from South Borneo with Mataram. Banteng was closest to Bali cattle from Nusa Penida and the longest was to Bali cattle from South Borneo. This finding indicates there is inbreeding in Bali cattle, therefore it needs to be concerned in bull rotation and semen distribution for increasing the Bali cattle performance.
Genetic Diversity and Relationship among Bali Cattle from Several Locations in Indonesia Based on ETH10 Microsatellite Marker Margawati, Endang Tri; Volkandari, Slamet Diah; Indriawati, Indriawati; Ridwan, Muhamad
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 4 (2018): DECEMBER 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.619 KB) | DOI: 10.14334/jitv.v23i4.1915

Abstract

Bali cattle is one of local beef cattle in Indonesia, up to present its performance indicated an inbreeding occurrence. This study was aimed to analyze the genetic diversity and relationship among Bali cattle from several locations in Indonesia based on ETH10 microsatellite marker. Ninety-four (94) DNA samples (89 Bali cattle; 5 Banteng) were analyzed. The Bali cattle samples were from 6 locations in Indonesia (15 Pulukan; 15 Nusa Penida; 14 Bima West Nusa Tenggara/WNT; 10 Mataram, WNT; 20 Riau; 15 South Borneo). DNA Banteng samples were collected from Prigen Malang of East Java. Microsatellite marker of ETH10 labelled HEX was used for amplification. Alleles were analyzed by using Cervus 3.0.7 and GenAlex 6.5. Result showed that there were five (5) alleles found in ETH10 marker i.e., 209; 213; 215; 217; and 219 bp. Average of observed (Ho) and expected (He) heterozygosity were 0.46±0.05 and 0.60±0.03, respectively. Five (5) out of 6 locations were in breeding occurrence except Bali cattle from Mataram was not inbreeding. The longest genetic relationship was between Bali cattle from Mataram and Riau whereas the closest distance was Bali cattle from South Borneo with Mataram. Banteng was closest to Bali cattle from Nusa Penida and the longest was to Bali cattle from South Borneo. This finding indicates there is inbreeding in Bali cattle, therefore it needs to be concerned in bull rotation and semen distribution for increasing the Bali cattle performance.
IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU [Identification of Jembrana Disease Virus by Using a Molecular Marker of env SU Gene in Bali Cattle] Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.055 KB) | DOI: 10.14203/beritabiologi.v12i2.534

Abstract

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.
RETRANSFORMATION AND EXPRESSION OF RECOMBINANT VIRAL PROTEIN OF JEMBRANA JSU AND JTat (JSU AND JTat) IN pGEX SYSTEM [Retransformasi dan Ekspresi Protein Virus Rekombinan JSU dan JTat Penyakit Jembrana dalam Sistem pGex] Margawati, Endang T; Utama, Andi; Indriawati, Indriawati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.793 KB) | DOI: 10.14203/beritabiologi.v9i1.802

Abstract

Genom virus penyakit Jembrana setidaknya memiliki 3 gen besar yang menyandi protein dan beberapa di antaranya diperlukan untuk replikasi virus. Protein JSU dan JTat diduga dapat menginduksi kekebalan yang protektif pada sapi Bali terhadap penyakit Jembrana sehingga keduanya sangat berpotensi untuk dipakai sebagai vaksin rekombinan. Penelitian ini dirancang untuk meretransformasi protein rekombinan JSU dan JTat ke dalam Escherichia coli menggunakan sistem pGEX. Konstruk JSU dan JTat dalam pGEX dikoleksi plasmidnya dengan metode miniprep dan kemudian diretranformasikan ke dalam E. coli strain BL21 dan DH5a. JSU dan JTat hasil retransformasi diekspresikan pada medium LB untuk skala produksi kecil dengan sistem pGEX. Hasil penelitian ini meminjukkan bahwa kedua JSU dan JTat hasil retransformasi ke dalam E. coli strain BL21 terlihat tumbuh lebih baik pada medium LB jika dibandingkan retransformasi ke dalam E. coli strain DH5a. Hasil retransformasi JSU dan JTat dikarakterisasi dan diidentifikasi dengan Western blotting dan tampak menunjukkan ukuran protein yang benar, yaitu protein rekombinan JSU berukuran 60kDa dan JTat berukuran 36,7kDa. Protein rekombinan JSU muncul dengan pita tunggal dan lebih jelas jika dibandingkan dengan protein JTat. Konsentrasi protein JSU sedikit lebih rendah (1,883 mg ml ) jika dibandingkan dengan JTat (l,981mg ml).Penelitian ini menunjukkan bahwa JSU pGEX masih tersimpan dan diekspresikan dengan baik, sementara JTat mungkin perlu dilakukan perakitan ulang untuk memantapkan ekspresinya.
Metode Sensitif untuk Identifikasi Pencemaran Babi pada Makanan Tanpa Diolah dengan Teknik Amplifikasi PCR Tri Margawati , Endang; Ridwan, Muhamad; Indriawati, Indriawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.117

Abstract

Pada era globalisasi akhir-akhir ini, tidak mungkin menghindar dari masuknya bahan makanan olahan atau tanpa diolah dari luar negeri. Tujuan penelitian ini yaitu menguji sensitivitas (kerentanan) teknik PCR untuk deteksi kandungan rendah kontaminan daging babi pada daging sapi mentah. Sebanyak 5 tingkat kontaminan daging babi (0,05; 0,10; 0,15; 0,20 dan 0,25%) dalam 5 gram berat total campuran daging telah diuji. Ke lima campuran daging dan 100% daging sapi serta 100% daging babi, dikoleksi DNA nya menggunakan kit DNA (QIAGEN). Satu pasang primer spesifik untuk porcine Leptin digunakan dalam amplifikasi DNA dengan kit PCR. Suhu annealing ditentukan dengan optimasi PCR terlebih dahulu. Dua siklus PCR (25 dan 35) diaplikasikan dalam amplifikasi. Produk PCR divisualisasi pada 1020% gradient PAGE untuk spesifik porcine Leptin. Hasil menunjukkan bahwa ukuran potongan Leptin (152pb) telah teridentifikasi pada ke lima sampel sampuran maupun pada control positif (pork), namun tidak terindikasi pada kontrol negatif (daging sapi). PCR dengan 35 siklus menghasilkan tampilan pita lebih baik dari 25 siklus. Studi ini menyarankan bahwa PCR dengan 35 siklus dapat digunakan sebagai metode cepat untuk identifikasi pencepamaran daging babi dengan dengan tingkat sensitivitas pencemaran daging babi sampai 0,05%.