Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ISOLATION AND TEST OF HETEROTROF BACTERIA ACTIVITY TO PATHOGEN BACTERIA FROM SEA DISTRICT SIAK REGENCY, RIAU PROVINCE Dinata, Rendy Lani; Feliatra, Feliatra; Yoswaty, Dessy
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT  Heterotrophic bacteria are bacteria that require organic carbon and inorganic nitrogen as an energy source, Research aims to determine the number of heterotrophic bacteria, knowing the results of its activity test against pathogenic bacteria (Vibrio sp, Aeromonas sp, Pseudomonas sp) and to know the species of heterotrophic bacteria through DNA test isolated from estuari waters of 15 ppt and the industrial estuary at coastal areas Sungai Kayu Ara  village with salinity 27 ppt, Siak District. Distribution of bacteria at station  higher than station 2 with value of nilai 7,04 x 107cfu / ml at station 1 to 6,46 x 107cfu / ml at station 2. Furthermore, through simple linear regression test known that the growth of heterotrophic bacteria on environmental parameters (DO, pH, temperature and salinity) categorized very weakly with the value of r = 0.10 in all parameters. Based on the antagonism test on pathogenic bacteria, it was found that there were 9 bacterial isolates that potentially inhibited the growth of the three pathogenic bacteria, namely: RM3, RM4, RM5, RM6, RM8, RM11, RL15, RL17 and RL 24 with inhibit zone ranges from 0.6 - 18mm, where the RM11 (12-15.3 mm) isolate as the best isolate in its inhibitory properties. By DNA test with 16S rRNA method and BLAST analysis, it was found that Bacillus safensis, Alcaligenes faecalis, Enterobacter sp, Enterococcus sp and Vagococcus sp species were identified as unidentified and possibly non-registered species in Genbank NCBI because it has a homology percentage of only 84%.                                                      Keywords: Heterotrophic Bacteria, Distribution, Antagonism, DNA
Isolation And Heterotrophic Bacteria Antagonism Test From Estuary Of The Siak River with Different Salinity Yanti, Syaputry Lita; Feliatra, Feliatra; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The research was conducted from July to September 2017 in Estuary of Siak River. The purpose of this research is to examine thepopulation of heterotrophic bacteria in Estuary of Siak River, and heterotrophic bacteria antagonism against pathogenic bacteria (Vibrio sp, Aeromonas sp and Pseudomonas sp). And than identified by phenotype and genotype. This research use survey method. The samples analisis were conducted at the Marine Microbiology Laboratory of Marine Science Department of Fisheries and Marine Faculty and DNA analisis conducted at the Genetic Laboratory of Biology Department Mathematic and Natural Sciences. The result of antagonism test from 25 isolate heterotrophic bacterial was found 10 isolates which have ability to inhibit the growth of pathogenic bacteria (Vibrio sp, Aeromonas sp and Pseudomonas sp). From Vibrio sp bacteria the mean of clear zone is 1P11 isolate of 20,3 mm and the smallest is 1P19 isolate of 5 mm. Aeromonas sp bacteria the largest clear zone is 2P20 of 9,3 mm and the smallest is 1P12 isolate of 3,3 mm. Pseudomonas sp bacteria the largest clear zone is 1P12 isolate of 17 mm and the smallest is 1P11 and 2P22 isolate of 8,3 mm. the result of molecular identification using PCR 16S rRNA method and BLAST analisis showed 10 isolates of heterotrophic bacteria having kinship with bacteria of the genus Bacillus that is Bacillus cereus (1P11, 1P12, 1P14, 1P15, 1P19, 2P20, 2P21, 2P23), Bacillus toyonensis (2P22) and Virgibacillus salarius (1P18). Keyword : Heterotrophic Bacteria, Isolation, Antagonism test, Identification
Isolation And Sensitivity Test of Heterotrophic Bacteria From Marine and Estuary Waters of Low Salinity In Siak against Pathogenic Bacteria Hamdani, Rizki; Feliatra, Feliatra; Nurrachmi, Irvina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT             Heterotrophic bacteria are a bacteria that use organic matter as a source of nutrients. It also has potential as antibacterial to pathogenic bacteria. The research was conducted from June to September 2017. The aim of this research was to determine the ability of bacterial isolates to inhibit the growth of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.) and determine the species of haterotrophic bacteria in the research area based on PCR method of 16S rRNA sequence. The results showed that twenty five pure bacteria isolates and only ten isolates could be sequenced. Bacterial isolates H2, H4, H8, H10, H12, H15, H16, H17 and H24 were able to inhibit the growth of pathogenic bacteria and were classified into three categories namely strong category (10-20 mm), medium category (5-10 mm) and weak category (≤ 5 mm). Based on the 16S rRNA analysis, it was found that ten identified isolates included Bacillus sp., Bacillus cereus, and Pseudomonas aeruginosa with a homology level of 81-99%. Based on the results of simple linear regression test showed the relationship between the number of heteretrophic bacteria with environmental parameters measured in the very weak category (r = 0,19). Keywords: heterotrophic bacteria, antibacterial, moleculer analysis, 16S rRNA 
ANTAGONISM HETEROTROPHIC BACTERIA ISOLATED FROM ESTUARY OF SIAK RIVER AND SETTLED SEA OF SIAK REGENCY AGAINST PATHOGENIC BACTERIA Zirma, Andrei Putra; Feliatra, Feliatra; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5, No 1 (2018): Wisuda April Tahun 2018
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   Heterotrophic bacteria is bacteria that use organic substances as its nutrition sources. Organic substances are obtained from the rest of other organisms, waste or substances contained in the body of other organisms. This study aims to determine the activity of heterotrophic bacteria against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.). Total amount of bacteria at station 1 was higher than station 2, those were 2.3 x 107 cfu / ml at station 1 and 1.8 x 107 cfu / ml at station 2. Statistical analysis using the simple linear regresion indicated that the correlation between the growth of heterotrophic bacteria on environmental parameters (DO, pH, temperature and salinity) was very weak of wich correlation value(r) was 0.20  for all parameters. Antagonism test against pathogenic bacteria found that there were 8 bacterial isolates wich potentially inhibited the growth of the three pathogenic bacteria, the isolates were A11, A12, A14, A19, A21, A22, A23 and A25 with the inhibition zone ranges 4,8 - 14,5 mm. From the DNA sequences with 16S rDNA method and BLAST analysis, the bacteria isolates were identified asKerstersia gyiorum, Kerstersia sp.,Enterococcus sp. and three isolates was unidentified possibly due to these species hadnot registered in GenBank or had never been identified beforeKey words: Heterotrophic Bacteria, Antagonism, Estuary, 16s rDNA
DEGRADATION ABILITY AND IDENTIFICATION OF OIL DEGRADATION BACTERIA BY 16S rRNA SEQUENCING Shaumi, Nurul; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
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ABSTRACTPollution in the marine environment caused by industrial activity, marine transportation, offshore mining and oil refineries, one of which comes from petroleum contamination. One of  efforts to prevent and to manage oil pollutionwas by the use of oil degrading bacteria. The objective of this research was to examine degradation ability of marine bacteria isolates and identify the bacterial isolate based on 16S rRNA sequence. The research was conducted from March to May 2018. The oil degradation analysis was conducted at Laboratory of Marine Microbiology,Faculty of Fishery and Marine Science, while the molecularidentification of bacteria was done in Genetics Laboratory, Faculty of Mathematics and Natural Sciences, University of Riau. The results showed that five isolates of oil degradation bacteria, selected(BM 1d, BM 7b, BM 7c, BM 14a and BM 14b) were able to degrade crude oil at different concentrations. The highest degradation at 1% concentration is indicated by BM 14b isolate, at 2% concentration was indicatedby BM 1d isolate and at 3% concentration was indicated BM 14b isolate. From the degradation analysis results, three isolates (BM 1d, BM 14a, BM 14b) have the highest degradation ability to determine the type of oil degrading bacteria. The molecular identification results by the 16S rRNA sequencing and BLAST analysis showed that BM 1d isolate were similar to Bacillus sp. (95%), BM 14a isolate were similar to Bacillus flexus (92%) and BM 14b isolate were similar to Bacillus cereus (98%). Keyword : Oil Degradation Bacteria , Crude Oil, 16S rRNA, Bacillus
CHARACTERIZATION OF LACTIC ACID BACTERIA FROM CINCALUK BASEDON16S rRNA SEQUENCE Ayu, Putri; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
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ABSTRACT Lactic acid bacteria (LAB) is a groupof bacteria frequently used as probiotic in aquaculture because of its beneficial effects on health, pathogenic bacteria prevention and increase of fish growth. LAB could be found in fermented fish products such as Cincaluk that is produced by people in Tembilahan and Bengkalis of Riau Province. The purpose of this research was to characterize LAB isolated from Cincaluk Tembilahan and Bengkalis genetically. This research was conducted from February until May 2018. Bacterial isolates were identified based on morphology, biochemical characters and 16S rRNA sequence. 24 isolates found were round, creamy white, Gram positive, did not produce indole, produced catalase, non motil, used citrate, did Methyl Red, produced H2S and gas, glucose, sucrose and lactose were fermented. Based on 16S rRNA sequence. Five of six isolates selected were identified as Lactobacillus plantarum (97-100% similarites) and one isolate was Bacillus cereus (94%) Keyword:Characteristic, Lactid Acid Bacteria, Cincaluk, Genetic,16S rRNA sequence 
Karakterisasi Molekuler Bakteri Probiotik Ikan Kerapu Bebek Berbasis Teknik 16S Ribosomal DNA Feliatra, Feliatra; Andrito, Wahyudi
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPTTangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.
DENSITY OF Escherichia coli BACTERIA AND HETEROTROPHIC BACTERIA IN DUMAI SEAWATER OF RIAUPROVINCE Qoriman, Anisa; Feliatra, Feliatra; Nursyirwani, Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT This research was conducted from April until May 2018. The purpose of this study was to calculate the density of Escherichia coli in sea water of Dumai, to determine the ratio of E. coli to heterotrophic bacteria and to examine the resistance of E. coli to antibiotics. In this study the determination of sampling points using purposive sampling method, was set intothree sampling points for each station. Density ofE.coliwas calculated by using the Most Probable Number (MPN) method and density total of heterotrophic bacteria by using spread plate count method. The result showed that densityE. colirangedfrom 2.37 x 104 – 1.1 x 105 CFU/ml. The highest MPN number ofE. coliwas found in stations 2 and 3, andthe lowest wasin station 5. The total number of heterotrophic bacteria ranged from 7.37 x 106 – 10.38 x 106 CFU/ml. The highest number of heterotrophic was foundin station 4 and the lowest was in station 5.The highest percentage ratio of density of E.colito heterotrophic bacteriawas in station 2(1.20%)and the lowest was in station 5 (0.32%).Resistance of E.coli toward chloramphenicol as antibiotic testedindicated that 7 isolateswere resistant with the inhibition zone ranged from 2.17 –10.70 mm, 9 isolates were categorized intointermediate with the inhibition zone ranged from 11.90–19.33 mm and 1 isolate was sensitive with the inhibition zone was 22.77 mm. Towardantibiotics penicillin andisoniazid, all bacterial isolates were resistant with the inhibition zone ranged from 0.80 - 4.70 mm and 1.50- 4.67 mm, respectively.Keyword: Density, Escherichia coli,MPN method, Heterotrophic Bacteria, Antibiotics 
DENSITY OF Vibrio sp. BACTERIA AND HETEROTROFIK BACTERIA IN DUMAISEA WATEROF RIAUPROVINCE Masrudy, Aan Deni; Feliatra, Feliatra; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This research was conducted from April until May 2018. The purpose of this study was to calculate the density of Vibrio sp. bacteria insea water of Dumai,to determine the ratio of Vibrio sp.toheterotrophicbacteria to examine the resistence of Vibrio sp. to antibiotics. In this study the determination of sampling points using purposive sampling method, was set into three sampling points for each station. Density total of Vibrio sp. and heterotrofik bacteria by using spread plate count method. The result showed that density Vibrio sp.ranged from 1.3 x 103 - 1.6 x 103 CFU / ml. the haignest number of Vibrio sp. wasfound in stations 4, and the lowest was in station 5.  The total number of heterotrophic bacteria ranged from 7.37 x 106 - 10.38 x 106 CFU / ml. the highest number of heterotrophic was found in station 4 in the lowest was in station 5. The hignest percentange ratio of the density of Vibrio sp.to heterotrophic bacteria was in station 5 0.0178% and the lowest was in station 1 0.0144%. resistance ofVibrio sp. toward Chloramphenicol as antibiotic tested indicatedthat 31 isolates were categorized into intermediate with the inhibition zone ranged from 12-19.8 mm, and 1 isolate was sensitive with the inhibition zone was 21 mm. Toward antibiotics penicilin and isoniazid, all bacterial isolates were resistant with the inhibition zone ranged from 1.6 - 9 mm and 0.9 - 6.2 mm, respectively. Keyword: Density, Vibrio sp,Heterotrophic Bacteria, Antibiotics 
DENSITY OF Pseudomonas sp. BACTERIA AND HETEROTROPHIC BACTERIA IN DUMAI SEAWATER OFRIAU PROVINCE Masdini, Alvira; Feliatra, Feliatra; Effendi, Irwan
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT Pseudomonas sp. is a hydrocarbonoclasticbacteria that is capable of degrading various types of hydrocarbons. Pseudomonas sp.including bacteria that have the potential to become pathogens. This research was conducted in April to May 2018. This study aims to calculate the density of Pseudomonas sp.bacteriaand heterotrophic bacteria in Dumai sea waters, comparison of the desity of Pseudomonas sp. bacteria to heterotrophic bacteria at each station and the resistance of Pseudomonas sp. to antibiotics. The result showed that densityPseudomonas sp. ranged from 2.25 x 105- 6.08 x 105 CFU/ml. The highest number of Pseudomonas sp.at station 1 and lowest at station 5. The total number of heterotrophic bacteria ranged from 7.37 x 106 - 10.38 x 106 CFU/ml. The highest number of heterotrophic bacteria at station 4 and the lowest at station 5.The highest percentage rasioof density of Pseudomonas sp. bacteri to heterotrophic bacteria was in station 1 (6.26%) and the lowest was in station 4 (2.86%). The results of isolation of bacteria obtained 25 pure isolates and tested for bacterial resistance to antobiotics. Results of isolation bacteria obtained 25 pure isolates and tested the resistance ofPseudomonassp. bacteriato antibiotics.Chloramphenicolantibiotic test results showed 2 isolates classified as sensitive with inhibition zone of 21,3 – 21,9 mm, 1 isolate including resistance with 9,7 mm inhibition zone, with a 22 isolates classified as intermediate with a inhibition zone of 9.7 – 19.6 mm. Two other antibiotics Penicillin and Isoniazid, all bacterial isolates were resistant with the inhibition zone range from 1.2 – 4.6 mm and 1 – 2.8 mm respectively Keyword: Density, Pseuomonas sp.,Heterotrophic Bacteria, Antibiotics