Helmi, T. Zahrial
JURNAL ILMIAH MAHASISWA VETERINER

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PENGARUH PAPARAN TIMBAL (Pb) TERHADAP LAJU PERTUMBUHAN IKAN NILA (Oreochromis nilloticus) Rahayu, Nur Inda; Rosmaidar, Rosmaidar; Hanafiah, M.; Karmil, T. Fadrial; Helmi, T. Zahrial; Daud, Razali
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.865 KB) | DOI: 10.21157/jim vet..v1i4.4757

Abstract

 Penelitian ini bertujuan mengetahui tingkat konsentrasi timbal   (Pb)  yang berpengaruh terhadap laju pertumbuhan ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 40 ekor dengan kriteria: sehat; bobot badan 15 – 18 gram; umur ± 2 bulan; jenis kelamin jantan. Penelitian ini menggunakan 4 kelompok perlakuan, P0 sebagai kontrol ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/L  dan pakan pelet, P2 diberikan paparan timbal 12,53 mg/L  dan pakan pelet dan P3 diberikan paparan timbal 25,06 mg/L  dan pakan pelet,  masing-masing perlakuan terdiri dari 10 ekor ikan nila. Perlakuan dilakukan selama 30 hari dan Pengukuran pertumbuhan ikan dilakukan setiap 10 hari sekali dengan cara menimbang bobot dan mengukur panjang tubuh setiap individu ikan. Hasil penelitian menunjukkan rata-rata panjang tubuh ikan nila P0 (9,45±1,06), P1 (8,89±0,90), P2 (8,86±0,87), dan P3 (8,66±0,85). Rata-rata berat ikan nila P0 (23,38±4,50), P1 (19,75±2,27), P2 (19,15±2,10), dan P3 (18,65±2,00). Laju pertumbuhan spesifik P0 (38,7%), P1 (8,3%), P2 (4,3%), dan P3 (3,3%). Laju pertumbuhan panjang harian individu ikan P0 (0,46 mm/d), P1 (0,27 mm/d), P2 (0,21 mm/d), dan P3 (0,19 mm/d). Kesimpulan dari penelitian bahwa konsentrasi timbal  dan lamanya paparan berpengaruh terhadap laju pertumbuhan ikan.  Konsentrasi timbal  yang sangat berpengaruh adalah 25,06 mg/L. ABSTRACTThe aims of this research was to find out to determine the level of Lead (Pb) concentration that affect the rate of growth tilapia (Oreochromis nilloticus). This study used 40 tilapia with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study used 4 treatment groups, P0 as control, fish fed only pellets, P1 was given lead exposure 6,26 mg/L and pellet, P2 was given lead exposure 12,53 mg/L and pellet and P3 was given lead exposure 25,06 mg/L and pellet. Each treatment consisted of 10 tilapia fish. Treatment carried out for 30 days and measure every 10 days. The results showed the average length of tilapia fish P0 (9,45±1,06b); P1 (8,89±0,90a); P2 (8,86±0,87a) and P3 (8,66±0,85). Average weight of tilapia fish P0 (23,38±4,50); P1 (19,75±2,27); P2 (19,15±2,10) and P3 (18,65±2,00). Spesific growth rate P0 (38,7%); P1 (8,3%); P2 (4,3%) and P3 (3,3%). The rate of long-term growth of individual fish P0 (0,46 mm/d); P1 (0,27 mm/d); P2 (0,21 mm/d) and P3 (0,19 mm/d). In conclusion, it showed that the concentration of lead (Pb) and the duration of exposure has affect the growth rate of fish. The influential concentration of lead is 25,06 mg/L.
17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate Dewi, Maryulia; Darmawi, Darmawi; Helmi, T. Zahrial; Erina, Erina; Gani, Basri; Eliawardani, Eliawardani; Azhar, Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.621 KB) | DOI: 10.21157/j.med.vet..v13i1.8884

Abstract

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.