HENNIVANDA, HENNIVANDA
JURNAL ILMIAH MAHASISWA VETERINER

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Resistensi Escherichia coli yang diisolasi dari telur Ayam di Beberapa Warung kopi kawasan Darussalam Kecamatan Syiah Kuala, Banda Aceh terhadap Antibiotik (Escherichia coli Resistance that Isolated from Chicken Eggs in Several Darussalam Coffee Shop Syiah Kuala Sub-district Area Banda Aceh of Antibiotics ALFURQAN, M.ARIF; RASTINA, RASTINA; HARIS, ABDUL; NURLIANA, NURLIANA; HENNIVANDA, HENNIVANDA; ERINA, ERINA
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
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Abstract

Penelitian ini bertujuan mengetahui resistensi beberapa jenis antibiotik terhadap Escherichia coli yang diisolasi dari telur ayam dari beberapa warung kopi kawasan Darussalam, Banda Aceh.Metode deteksi E.coli mengacu pada Bacteriological Analytic Manual (BAM) dan pengujian kepekaan E.coli terhadap antibiotik dilakukan dengan metode difusi cakram.Penelitian ini menggunakan 21 telur ayam yang dari tujuh warung kopi.Antibiotik yang digunakan yaitu ampisilin, gentamisin, streptomisin, siprofloksasin, eritromisin, sulfametoksasol dan tetrasiklin.Data dianalisis secara deskriptif. Hasil penelitian ini menunjukkan ampisilin, eritromisin dan sulfametoksasol yang resisten terhadap 12 koloniE.coli, tetrasiklin dan streptomisin resisten terhadap 11 koloni E.coli, dan gentamisin resisten terhadap 2 koloni E.coli. Berdasarkan penelitian ini dapat disimpulkan E. coli yang diisolasi dari telur ayam yang dijual di warung kopi kawasan Darussalam Kecamatan Syiah Kuala Banda Aceh menunjukkan tingkat resistensi yang sangat tinggi yaitu ampisilin 100%, eritromisin 100%, tetrasiklin 92%, streptomisin 92% dan sulfametoksasol 100%) dan tidak resisten terhadap antibiotik jenis siprofloksasin dan gentamisin.Kata kunci: Esherichia coli, antibiotik, resisten, telur ayam.ABSTRACTThe aim of this study was to determine of antibiotic resistance in some types of Escherichia coli which isolated from Chicken Eggs in several Darussalam Coffee shop Banda Aceh Area. The Detection test of E.coli that reference to Bacteriological Analytical Manual (BAM) method and sensitivity test for E. coli to antibiotics is done by disc diffusion method. This study used 21 Chicken Eggs samples that collected from 7 coffee shop, the antibiotics that used were ampicillin, gentamycin, streptomycin, ciprofloxacin, erythromycin, sulfametoxazole, and tetracycline. The data were analyzed descriptively. The results of this study showed resistance to the ampicillin antibiotic, erythromycin and sulfametoxazole to as well as 12 E. coli colonies, 11 E. coli colonies for tetracycline and streptomycin, and 2 E. coli colonies for gentamycin. The conclution is this study showed a fairly high level of resistance on the egg that isolated from several Darussalam coffee shop area banda aceh, there is resistance to ampicilin antibiotic 100%, eritromycin 100%, tetracycline 92%, streptomisin 92% and sulfametoxazole 100%, and not resistance of ciprofloxacyn and gentamycin antibiotics.Keywords : Esherichia coli, antibiotic, resistance, Chicken Eggs.
DETEKSI Salmonella sp PADA SALURAN PENCERNAAN KURA-KURA AMBON (Cuora amboinensis) (Detection of Salmonella sp in Gastrointestinal Tract of Ambon Turtle (Cuora amboinensis)) Erina, Erina; Dewi, Karunita; Sutriana, Amalia; Fakhrurrazi, Fakhrurrazi; Ismail, Ismail; Hennivanda, Hennivanda
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 2 (2019): FEBRUARI-APRIL
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Abstract

ABSTRAK            Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri Salmonella sp pada saluran pencernaan kura-kura ambon (Cuora amboinensis). Sampel penelitian menggunakan 6 ekor kura-kura ambon (Cuora amboinensis) yang dipelihara oleh masyarakat. Penelitian ini menggunakan metode Carter dengan cara mengambil swab kloaka kura-kura ambon (Cuora amboinensis) lalu diinokulasi pada media selenite cystine broth (SCB) kemudian ditanam pada media selektif Salmonella Shigella agar (SSA) dan diamati secara makroskopis morfologi koloninya lalu dilakukan pewarnaan Gram untuk pengamatan secara mikroskopis. Selanjutnya dilakukan uji biokimia IMVIC (indol, methyl red, Voges Proskauer, sulfid indol motility, Simmon’s citrate), triple sugar iron agar dan uji gula-gula (glukosa, sukrosa, laktosa dan manitol). Hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa 6 sampel kura-kura ambon (Cuora amboinensis) yang diteliti positif terdapat bakteri Salmonella sp di dalam saluran pencernaannya. Hal ini mengindikasikan bahwa kura-kura ambon (Cuora amboinensis) berpotensi untuk menjadi sumber penularan salmonellosis pada manusia.ABSTRACT            The purpose of this study was to detect the existence of Salmonella sp in ambon turtle (Cuora amboinensis) gastrointestinal tract. The sample used was cloacal swab sample taken from 6 ambon turtles reared by society. This research using Carter method by inoculating cloacal swab on selenite cystine broth, planting on a selective medium Salmonella Shigella agar, observing colonies morphology macroscopically, and conducting Gram staining. After that, the biochemistry IMVIC test (indole, methyl red, Voges Proskauer, sulfide indole motility, Simmon’s citrate), triple sugar iron agar, and sugars test (glucose, sucrose, lactose and mannitol) were performed. The data were analyzed descriptively. The research showed that 6 cloacal swab samples of ambon turtle were positive containing Salmonella bacteria in their gastrointestinal tract. Its indicated that the ambon turtle potential to become the source of salmonellosis in human.
ISOLASI DAN IDENTIFIKASI BAKTERI GRAM NEGATIF PADA AMBING KAMBING PERANAKAN ETAWA (PE) (Isolation and Identification of Gram Negative Bacteria on the Udder of Etawa Crossbred (PE) Goat) Ginting, Septian Tri Mulyana; Helmi, Teuku Zahrial; Darmawi, Darmawi; Dewi, Maryulia; Erina, Erina; Daud, Razali; Hennivanda, Hennivanda
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
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Abstract

ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Gram negatif pada ambing kambing peranakan etawa (PE). Sampel yang digunakan pada penelitian ini terdiri dari swab ambing dari 5 ekor kambing PE. Sampel dibiakkan pada media nutrient broth lalu diinkubasikan pada suhu 37°C selama 24 jam. Isolasi dan identifikasi dilakukan dengan metode Carter yang meliputi pengamatan morfologi koloni, pewarnaan Gram, penanaman pada media diferensial dan selektif serta uji biokimia. Hasil isolasi dan identifikasi pada 5 sampel swab ambing kambing PE didapatkan dua koloni bakteri Gram negatif yaitu Escherichia coli dan bakteri X yang belum teridentifikasi melalui uji-uji yang dilakukan. Berdasarkan hasil tersebut dapat disimpulkan bahwa bakteri Gram negatif pada ambing kambing PE adalah Escherichia coli dan bakteri X yang belum teridentifikasi.Kata kunci: kambing PE, ambing, bakteri Gram negatif. ABSTRACTThis study aimed to isolate and identify Gram-negative bacteria on udder of etawa crossbred (PE) goat. Swab samples were obtained from 5 PE goats udder. Swab samples were cultured in nutrient broth media and incubated at 37°C for 24 hours. Isolation and identification were performed by Carter method, followed by observation of colony morphology, Gram staining, cultured on differential and selective media, and biochemical tests were performed. The result showed that there were two colonies of Gram negative bacteria Escherichia coli and the other colony has not been identified yet with this tests. Therefore it can be concluded that the Gram-negative bacteria found on udder of PE goat were Escherichia coli and bacteria X that has not been identified by using this method.Keywords:etawa crossbred goat, udder, Gram-negative bacteria.