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Ichrom Nahzi, Muhammad Yanuar
FKG Unlam

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PENGARUH EKSTRAK KULIT MANGGIS (GARCINIA MANGOSTANA L.) TERHADAP JUMLAH SEL LIMFOSIT PADA INFLAMASI PULPA Studi In Vivo pada Gigi Molar Rahang Atas Tikus Putih Wistar Jantan

Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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Abstract

ABSTRACK  Background: Mangosteen is one of Indonesian’s favorite. Mangosteen pericarp extract contains saponin, tannin, flavonoid, xanthone and its derivatives, alpha-mangosteen, beta-mangosteen, and gamma-mangosteen, which have anti-inflammatory properties. Purpose: The aim of this study is to assess the effect of mangosteen pericarp extract on lymphocytes count in pulp inflammation and compare it to calcium hydroxide on day 1, 3, 5, and 7. Methods: This study was true experimental with pretest-posttest with control group design. Samples used were 39 white wistar (Rattus novergicus) rats divided into 3 groups of mangosteen pericarp extract treatment group, calcium hydroxide treatment group and no treatment group. Lymphocytes count was observed microscopically in zig zag fields of view. Result: The result presented mean scoring of lymphocytes in mangosteen pericarp extract treatment group as 3,67 on day 1, 6 on day 3, 11 on day 5, and 7,67 on day 7. Lymphocytes count reached its peak on day 5. Two way ANOVA and Post Hoc LSD tests indicated that there was significant difference between mangosteen pericarp extract treatment group and no treatment group, between mangosteen pericarp extract treatment group and calcium hydroxide treatment group, and between calcium hydroxide treatment group and no treatment group. Conclusion: There was a significant effect of mangosteen pericarp extract on lymphocytes count in wistar rats’ pulp inflammation compared to calcium hydroxide in decreasing lymphocytes count.  Keywords: pulp inflammation, lymphocytes, mangosteen pericarp extract, calcium hydroxide, antiinflammatory, pulp capping.  ABSTRAK  Latar Belakang: Manggis merupakan buah yang digemari oleh masyarakat Indonesia. Ekstrak kulit manggis mengandung saponin, tanin, flavonoid, xanthone dengan turunannya alpha-mangostin, beta-mangostin, dan gammamangostin, yang berfungsi sebagai anti-inflamasi. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit manggis terhadap jumlah sel limfosit pada proses inflamasi pulpa dan membandingkannya dengan kalsium hidroksida pada hari 1, 3, 5 dan 7. Metode: Jenis penelitian ini merupakan eksperimental murni dengan rancangan pretest-posttest with control group. Penelitian ini menggunakan 39 tikus putih (Rattus norvegicus) wistar yang dibagi menjadi 3 kelompok, yaitu kelompok perlakuan ekstrak kulit manggis, kelompok kalsium hidroksida, dan kelompok tanpa obat. Jumlah limfosit dilihat secara mikroskopis dan dihitung secara zig-zag. Hasil: Hasil penelitian menunjukan skoring rata-rata jumlah sel limfosit perlakuan ekstrak kulit manggis hari 1 (3,67), hari 3 (6), hari 5 (11), dan hari 7 (7,67). Jumlah sel limfosit mencapai puncak pada hari ke-5. Hasil uji Two Way ANOVA dan uji Post hoc LSD menunjukkan bahwa terdapat perbedaan bermakna antara kelompok ekstrak kulit manggis dan tanpa obat, antara kelompok ekstrak kulit manggis dan kalsium hidroksida dan antara kalsium hidroksida dan tanpa obat. Kesimpulan: Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa pemberian ekstrak kulit manggis berpengaruh terhadap jumlah sel limfosit pada inflamasi pulpa gigi tikus wistar dibandingkan kalsium hidroksida dilihat dari penurunan jumlah sel limfosit pada kelompok ekstrak kulit manggis dibandingkan kelompok kalsium hidroksida.

EFEKTIVITAS DAYA HAMBAT BAKTERI EKSTRAK BAWANG DAYAK TERSTANDARISASI FLAVONOID TERHADAP Enterococcus Faecalis (In vitro)

Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Background: Enterococcus faecalis is a bacterium which most often found on the wall of the root canal after the root canal treatment. It can be eliminated by NaOCl solution with high concentrations of 5,25%, this can cause toxic effects of the network around it. Dayak onion (Eleutherine palmifolia (L) Merr ) has antibacterial substances resulting active compound content one of it is flavonoid. Purpose: This study aims to determine the differences antibacterial activity of Dayak onion bulb which contains flavonoid compound on the growth of Enterococcus faecalis. Method: This experimental research using post test only with group design with 6 treatments groups, namely Dayak onion bulb extract 20 mg/ml, 40 mg/ml, 60 mg/ml and 80 mg/ml with 5,25% NaOCl as positive control and ethanol 96%as negative control. Result: The results of calculation inhibitory zone obtained the most effective concentration is 80 mg/ml of 21,314 mm,which is the category of high inhibitory zone, but no bacterial growth higher than the NaOCl 5,25% with a mean inhibition zone is 24,416 mm. The analysis data using shpiro-wilk to test a normality test and homogeneity test using levene’s test data showed normal and homogeneous (p<0,05). The analysis data is using one way Anova test that show there is significant differences p=0,000 (p<0,05), then further post hoc LSD test showed significant differences between concentrations of extract of Dayak onion bulb, NaOCl 5,25% and ethanol 96% p=0,000 (p<0,05). Conclusion: there are differences in the antibacterial activity of dayak onion bulb which contains flavonoid compounds on the growth of enterococcus faecalis and inhibitory zone obtained the most effective concentration is 80 mg/ml of 21,314 mm, but no bacterial growth higher than the NaOCl 5,25% with a mean inhibition zone is 24,416 mm.

THE EFFECT OF THERMOCYCLING TEST ON THE DIAMETRAL TENSILE STRENGTH VALUE OF BULK FILL RESIN COMPOSITE

Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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      Background:. Bulk fill resin composite can be applied and light-cured to depths of 4 mms at once. Varying temperature changes in the oral cavity that  caused by the comsumption cold or hot food and beverage may  cause stress on the resin composite material resulting in restoration failure. Temperature changes at 5°C and 55ºC may decrease the mechanical properties of resin composite, one of which is the diametral tensile strength. Purpose: to analyze the thermocycling test effect using 1500 and 3000 cycles to the  diametral tensile strength value. Method: This study was purely experimental post test-only with control design. Twenty four bulk fill resin composite samples were divided into 3 groups, which are control group didn’t tested thermocycling, second group treatment were tested thermocycling 1500 cycles and third group were tested thermocycling 3000 cycles.Diametral tensile strength was tested with universal testiimg machine and analyzed by One Way Annova. Result: mean value of diametral tensile strength bulk-fill resin composite of control group 42.35± 4.08 MPa, group thermocycling 1500 cycles 42.25 ± 2.26 MPa, and group thermocycling 3000 cycles 39,98±1.84  MPa. there are no significant difference in diametral tensile strength values of bulk-fill resin composite between thermocycling test group and control group. Conclusion: Thermocycling test 1500 cycle and 3000 cycles to composite resin not altered the diametral tensile strength value.

UJI EFEKTIVITAS ANTIBAKTERI AIR PERASAN JERUK NIPIS (CITRUS AURANTIFOLIA) SEBAGAI BAHAN IRIGASI SALURAN AKAR ALAMI TERHADAP PERTUMBUHAN ENTEROCOCCUS FAECALIS IN VITRO

Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACT Background: Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity. Acid substance in lime juice is coagulant source. Citric acid in lime juice has the quality to prevent bacterial and fungal growth. Enterococcus faecalis is known as the most resistant species in oral cavity and commonly found in post-root canal treatment cases. E.faecalis was reported in 20 of 30 persistent infected endodontic teeth after root canal treatment. The aim of this study to assess lime (Citrus aurantifolia) juice’s in vitro inhibition activity against Enterococcus faecalis. Purpose: This study used laboratory experimental method with postest only control group design using total random sampling; consisted of 6 treatments and 5 times repetition. Methods: Antibacterial activity test was performed using diffusion method. Data was analyzed using one way anova with confidence interval of 95% and the result presented that there’s a significant difference between β5%, 50%, 75%, 100% lime juice treatment groups and 3% hydrogen peroxide treatment group. Result:Based on the result of post hoc LSD, the conclusion of this study was 100% lime juice has better inhibition activity than 25%, 50%, 75% lime juice and 3% hydrogen peroxide. Conclusion: There was a difference of antibacterial effectiveness between lime juice and 3% H2O2 on Enterococcus faecalis bacterial growth observed from their inhibition zones.  ABSTRAK Latar Belakang: Jeruk Nipis (Citrus aurantifolia) merupakan salah satu tanaman yang mempunyai aktivitas antimikroba. Kandungan asam pada air perasan jeruk nipis merupakan sumber koagulan. Senyawa asam sitrat dalam air perasan jeruk nipis mampu mencegah pertumbuhan bakteri dan jamur. Enterococcus faecalis dikenal sebagai spesies yang paling resisten pada rongga mulut dan paling sering ditemukan pada kasus setelah perawatan saluran akar. E.faecalis ditemukan sebanyak 20 dari 30 kasus infeksi endodontik yang persisten pada gigi yang telah dilakukan perawatan saluran akar. Tujuan: Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Enterococcus faecalis secara in vitro. Metode: Penelitian ini dilakukan dengan metoda eksperimental laboratorium; postest only control group design dengan rancangan acak lengkap menggunakan 6 perlakuan dan 5 kali pengulangan. Metode uji aktivitas antibakteri menggunakan metode difusi. Analisis data menggunakan uji one way anova 95% menunjukkan terdapat perbedaan bermakna antara perlakuan air perasan jeruk nipis konsentrasi 25%, 50%, 75%, 100% dan perlakuan hidrogen peroksida 3%. Hasil: Pada hasil uji post hoc LSD diambil kesimpulan perlakuan air perasan jeruk nipis konsentrasi 100% memiliki efek daya hambat lebih baik dibandingkan  konsentrasi di bawahnya dan dibandingkan dengan perlakuan hidrogen peroksida 3%. Kesimpulan: Terdapat perbedaan efektivitas antibakteri dari air perasan jeruk nipis dan H2O2 3% terhadap pertumbuhan bakteri Enterococcus faecalis dilihat dari zona hambat.

PERBANDINGAN JARAK PENYINARAN DAN KETEBALAN BAHAN TERHADAP KEKERASAN PERMUKAAN RESIN KOMPOSIT TIPE BULK FILL

Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Abstract

Background: The polymerisation process is a process of polymer formation of a combination of several monomers. This is due to the incomplete polymerization, which affects the consistency and durability of the composite resin in the oral cavity. The maximum surface can result in composite resin are not able to withstand the pressure generated during mastication it can undergo cracking (rupture) or off the tooth. Purpose: To compare the material thickness and curing light distance of bulk-fill composite resin to determine its’ surface consistency. Method: Laboratorium experimental study with a post-test only with control group design. A total of 63 composite resin pieces were used as samples and divided into 9 treatment groups with different curing light distance (0 mm, 2 mm, 5 mm) and thickness (2 mm, 4 mm, 6 mm). Data were analyzed with One-Way ANOVA with a significance value of 0,000 (p<0,05). Post-Hoc Tuckey analysis with a p(<0,05) was used to determine the difference between two groups. Result: Post Hoc Tuckey analysis resulted in a significant value (p<0,05), which shows that there is a significant difference between each group and in the result show group with the thickness 2 mm and light distance 0 mm was the highest score. Conclusion: There is a comparison between curing light distance and material thickness towards surface consistency of Bulk Fill Composite Resin with thickness 2 mm and 4 mm with curing light distance 0 mm and 2 mm.

PENGARUH EKSTRAK KULIT MANGGIS (Garcinia mangostana Linn.) TERHADAP JUMLAH MAKROFAG PADA INFLAMASI PULPA Studi In Vivo Pada Gigi Molar Rahang Atas Tikus (Rattus norvegicus) Wistar Jantan

Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACK  Background: Treatment of dental pulp is a basic treatment in dentistry. Nowadays dental pulp treatment used adhesive resin substance, but adhesive resin substance is irritative and expensive thus alternative substance is needed to use. Pericarp of mangosteen containing to be rich in various pharmacys and antioxidant activity so that called queen of fruits. Xanthone, flavonoid, tanin, and saponin compound can working as antiinflammatory that very important in open dental pulp healing. Purpose: This research to investigate the effect pericarp of mangosteen extract to amount of macrophag on pulp response inflammatory and compare with calcium hydroxide. Methods: This study used true experimental method with post-test only group design and simple random sampling, consist of 39 male wistar rats were divided into 3 groups.  Perforated each dental pulp rats were treated with pericarp of mangosteen extract as treatment group, with calcium hydroxide as positive control group, and no treatment indeed (no medicine) as negative control group. On the day 1 to day 7, sampels for hystological analysis should be done after treatment, inflammatory reactions occured in all of groups. Result: Inflammatory reactions reckoned from scoring of macrophage. Two way Anova and Post Hoc LSD test indicated that pericarp of mangosteen extract more effective than calcium hydroxide in decrease scoring of macrophage. Based on research could be concluded that significantly the treatment process pericarp of mangosteen extract could be usefull to decrease scoring of macrophage in pulp inflammatory.  Keywords: open dental pulp, pulp inflammatory, macrophage, pericarp of mangosteen, calcium hydroxide  ABSTRAK   Latar Belakang: Perawatan pulpa terbuka merupakan perawatan dasar dalam kedokteran gigi. Perawatan pulpa terbuka pada saat ini dengan menggunakan bahan resin adesif, namun  resin adesif bersifat iritatif dan  mahal sehingga diperlukan bahan alternatif yang lebih aman digunakan. Kulit manggis mengandung senyawa yang memiliki berbagai aktivitas farmakologi dan antioksidan sehingga disebut queen of fruits. Senyawa golongan xanton, flavonoid, tanin, dan saponin dalam kulit buah manggis dapat berfungsi sebagai antiinflamasi yang penting dalam penyembuhan pulpa terbuka. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit manggis terhadap jumlah makrofag pada inflamasi pulpa dan membandingkannya dengan kalsium hidroksida. Metode: Penelitian ini merupakan penelitian eksperimental murni dengan post-test only group design, menggunakan Rancangan Acak Sederhana, terdiri dari 39 tikus Wistar jantan yang dibagi menjadi 3 kelompok. Pulpa gigi tikus diperforasi yang kemudian diberi perlakuan ekstrak kulit manggis sebagai kelompok perlakuan, dengan diberikan kalsium hidroksida sebagai kelompok kontrol positif, dan tidak diberikan aplikasi apapun (tanpa obat) sebagai kelompok kontrol negatif. Sampel dianalisis secara histologis pada hari ke -1 hingga hari ke -7 setelah aplikasi, reaksi inflamasi terjadi pada seluruh kelompok. Hasil: Reaksi inflamasi dihitung dari jumlah makrofag. Uji two way Anova dan uji Post Hoc LSD menunjukkan bahwa ekstrak kulit manggis lebih efektif menurunkan jumlah makrofag dibandingkan kalsium hidroksida. Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa pemberian ekstrak kulit manggis secara bermakna dapat menurunkan jumlah makrofag pada inflamasi pulpa. Kata-kata kunci: pulpa terbuka, inflamasi pulpa, makrofag, kulit manggis, kalsium hidroksida

PENGARUH EKSTRAK KULIT MANGGIS (GARCINIA MANGOSTANA L.) TERHADAP JUMLAH NEUTROFIL PADA INFLAMASI PULPA Studi In Vivo pada Tikus Wistar Jantan

Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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Abstract

ABSTRACK  Background:Indonesia has various numbers of plants with medicinal contents; one of them is mangosteen (Garcinia mangostana L.). Mangosteen pericarp which is regarded as waste turns out to have benefits to health. Mangosteen pericarp extract contains chemical substances such as saponin, tannin, flavonoid, steroid, quinon, and xanthone. These substances have many benefits, one of them is antiinflammatory properties.Purpose: The aim of this study was to assess the effect of mangosteen pericarp extract on neutrophils count in wistar rats’ pulp inflammation. Methods:This study was true experimental with post-test only with control design. Samples used were 39 male wistar rats divided into 3 treatment groups: no treatment group (negative control), mangosteen pericarp extract treatment group, and calcium hydroxide treatment group (positive control). Samples were analyzed histopathologically on day 1, 3, 5, and 7.Result: The result presented mean scoring of neutrophils in mangosteen pericarp extract treatment group as 13,33 on day 1, 10,00 on day 3, 4,33 on day 5 and 2,33 on day 7. Two way ANOVA and Post Hoc LSD tests indicated that there was a significant difference between mangosteen pericarp extract treatment group and no treatment group and calcium hydroxide treatment group with p value of < 0,05. Conclusion:There was a significant effect of mangosteen pericarp extract on neutrophils count in wistar rats’ pulp inflammation compared to calcium hydroxide in decreasing neutrophils count.  Keywords: mangosteen pericarp extract, Garciniamangostana L., anti-inflammatory, neutrophils, pulp inflammation,pulp capping.  ABSTRAK  Latar Belakang: Indonesia merupakan negara yang memiliki berbagai jenis tanaman yang berguna sebagai tanaman obat, salah satunya adalah buah manggis (Garcinia mangostana L.). Kulit buah manggis yang selama ini dibuang ternyata memiliki banyak manfaat bagi kesehatan. Kulit buah manggis mempunyai kandungan kimia berupa saponin, tanin, flavonoid, steroid, kuinon, dan xanthone. Kandungan tersebut memiliki banyak manfaat salah satunya sebagai antiinflamasi.Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit manggis (Garcinia mangostana L.) terhadap jumlah neutrofil pada inflamasi pulpa gigi tikus wistar.Metode: Penelitian ini merupakan eksperimental murni dengan post-test only with control design. Sampel terdiri atas 39 tikus Wistar dengan jenis kelamin jantan yang dibagi menjadi 3 kelompok perlakuan yaitu kelompok tanpa obat (kontrol negatif), kelompok ekstrak kulit manggis (perlakuan) dan kelompok kalsium hidroksida (kontrol positif). Sampel dianalisis secara histologis pada hari ke-1, 3, 5 dan 7. Hasil penelitian menunjukan nilai rata-rata jumlah neutrofil ekstrak kulit manggis adalah 13,33 pada hari ke- 1, 10,00  pada hari ke-3, 4,33 pada hari ke-5 dan 2,33 pada hari ke 7.Hasil: Hasil two way ANOVA dan Post Hoc LSD menunjukan bahwa terdapat perbedaan bermakna antara kelompok ekstrak kulit manggis dengan kelompok tanpa obat dan kelompok kalsium hidroksida dengan nilai p<0,05.Kesimpulan: Terdapat pengaruh ekstrak kulit manggis (Garcinia mangostana L.) terhadap jumlah neutrofil pada inflamasi pulpa gigi tikus wistardibandingkan kalsium hidroksida yaitu terjadi penurunan jumlah neutrofil pada kelompok ekstrak kulit manggis dibanding kelompok kalsium hidroksida.Kata-kata kunci:  ekstrak kulit manggis, Garcinia mangostana L. antiinflamasi, neutrofil, inflamasi pulpa,pulp capping

KEBOCORAN MIKRO AKIBAT EFEK SUHU TERHADAP PENGERUTAN KOMPOSIT NANOHYBRID

Dentino Vol 1, No 2 (2016)
Publisher : FKG Unlam

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ABSTRACTBackground: Composite is broadly used by dental practitioners as restoration material to treat caries. Nanohybrid composite is a type of composite composed of nano-sized filler combined with larger filler. Polymerization shrinkage commonly occurs in every restoration using composite, resulting in a gap between cavity margin and teeth structure, which eventually leads to microleakage. Purpose: The purpose of this study was to assess and measure the rate of nanohybrid composite microleakage as a result of temperature change from 5ºC to 60ºC. Methods: This study was true experimental with post test-only with control design. Specimens used were 20 maxillary premolars, divided into 2 groups: 5ºC to 60ºC temperature change treatment group and control group (without temperature change, sitting idly at 37ºC).  Results: Mean scoring of treatment group was 3 and control group was 1,9. Conclusion: It can be concluded that independent samples T-test presented significant difference between treatment group and control group.  ABSTRAKLatar Belakang: Komposit merupakan suatu bahan restorasi yang biasa digunakan oleh dokter gigi untuk menumpat gigi yang karies. Komposit nanohybrid merupakan salah satu jenis komposit yang memiliki komposisi filler berukuran nano dan digabung dengan filler yang berukuran besar. Pengerutan polimerisasi merupakan hal yang selalu terjadi pada setiap penumpatan dengan bahan komposit. Akibat dari pengerutan adalah terbentuknya celah antara tepi kavitas dan struktur gigi, hal tersebut akan menyebabkan kebocoran mikro. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui adanya kebocoran mikro pada komposit nanohybrid akibat perubahan suhu 5ºC ke 60ºC serta mengukur besar kebocoran mikro yang terjadi. Metode: Jenis penelitian ini merupakan eksperimental murni dengan rancangan post test-only with control design. Penelitian ini menggunakan gigi premolar rahang atas sebanyak 20 buah yang dibagi menjadi 2 kelompok, yaitu kelompok perlakuan perubahan suhu 5ºC ke 60ºC, dan kontrol yang tidak dilakukan perubahan suhu atau didiamkan pada suhu 37ºC. Hasil: Rata-rata skor untuk kelompok perlakuan adalah 3 dan kelompok kontrol adalah 1,9. Kesimpulan: Dapat disimpulkan bahwa hasil uji T-test tidak berpasangan  menunjukkan perbedaan yang bermakna antara kelompok perlakuan dan kelompok kontrol.

KEKERASAN PERMUKAAN RESIN-MODIFIED GLASS IONOMER CEMENT SETELAH PERENDAMAN DALAM AIR SUNGAI (Penelitian Menggunakan Air Sungai Desa Anjir Pasar, Barito Kuala, Kalimantan Selatan)

Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

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ABSTRACTBackground: Resin-modified glass ionomer cement is a hybrid form of glass ionomer cement and resin. Added resin (HEMA) in this material allows its characteristics to improve, especially its resistance of acid. The water of Desa Anjir Pasar River has acidic nature with pH as low as 3-5. Purpose: The aim of this study is to find surface hardness difference of resin modified glass ionomer cement after it was soaked in the water of Anjir Pasar Village River compared to aquadest-soaked materials. Method: The study used resin-modified-glass ionomer cement specimens prepared in cylindrical-shaped with diameter of 10 mm and thickness of 2 mm. One group was soaked in aquadest (control) and another group in river water with pH of 3 for 4.5 days (equivalent to 3 years of exposure) before measuring the surface hardness of each group. Data was analyzed using parametric analysis Independent T-Test 95% (α = 0.05) which presented p value = 0.003 (p<0.05). Result: The result indicated that there was a significant difference of surface hardness between resin modified glass ionomer cement soaked in the water of Anjir Pasar Village River with pH of 3 for 4.5 days and in the aquadest. Conclusion: Based in this study there is a great differences of hardness on resin-modified glass ionomer cement materials that are immersed in river water to sterilize aquades.Keywords: Resin-Modified Glass Ionomer Cement, river water, surface hardness

EFFECT OF DIAMETRAL TENSILE STRENGTH OF BULK FILL COMPOSITE RESIN WITH THE ADDITION OF SUGARCANE WASTE FIBER

Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Backgrounds: Bulk fill composite resin is a composite resin type which can be polymerized as deep as 4 mm and is indicated for posterior tooth restoration. Most posterior tooth restorations become fractured because of the composite resins that are not able to withstand the tensile stress that happens. Efforts to increase diametral tensile strength is by adding fiber. The use of natural fibers can be an alternative because it is environmentally friendly and inexpensive. One of the natural fibers is fiber bagasse (Saccharum Officinarum L.). Purpose: To know the influence of composite resin diametral tensile strength value in bulk fill  with the addition of bagasse fiber. Methods: Experimental laboratory studies with post-test designs only with control group designs. The experiment of consist 36 samples which divided into 3 groups. Group I non-fiber bulk fill resin composite, group II filling composite with bagasse fibers and group III filling composite resins with glass fibers as a control group. The sample are immersed in saline solution and incubated for 24 hours at 37° C. Then sample tested its diametrically tensile strength using a Universal Testing Machine. Data analysis used One Way Anova test with significance value of 0,001 (p<0,05), followed by Post Hoc Bonferroni. Results: Post Hoc Bonfferoni test obtained p value= 0.001 (p<0.05), which means there are significant differences between the three groups where the group with the addition of glass fiber obtained the highest diametral tensile strength value. Conclusion: the addition of bagasse fiber affects the diametral tensile strength and can increase the diametral tensile strength of the bulk fill composite resin.