Yulistiani, Dwi
IRIAP

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In Vitro protein digestibility and fermentability of mulberry (Morus alba)-Leucaena foliage mixed feed Yulistiani, Dwi; Jelan, Z. A.; Liang, J. B.
Indonesian Journal of Animal and Veterinary Sciences Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.605 KB) | DOI: 10.14334/jitv.v21i1.1352

Abstract

This experiment was carried out to determine the effect of mulberry-leucaena foliage mixed feed on protein digestibility and VFA production using an in vitro gas production study. Mulberry was mixed with one of 2 leucaena varieties (Leucaena leucocephala hybrid and Leucaena leucocephala local) at 3 levels (0, 25 and 50%). Study was conducted in completely randomized design. Mulberry foliage, leucaena and mixtures of mulberry-leucaena were incubated for 24 hours in glass syringes. Parameter recorded were gas production, in vitro true dry matter digestibility (IVTDMD), in vitro N digestibility (IVND) and VFA production. Results of the study showed that supplementation of leucaena to mulberry decreased IVOMD, gas production and protein digestibility in the rumen buffered medium. The mixture of mulberry and leucaena hybrid at ratio 50% resulted in the lowest IVND than other treatment. However it increased protein digestibility in acid pepsin incubation as an estimate of protein availability in intestine. Gas production decreased in mulberry leucaena mixtures was followed by decreased total VFA production. Tannin derived from leucaena hybrid supplementation to mulberry at ratio 1 : 1 was most effective to decrease protein digestion in the rumen and to increase protein digestibility in acid pepsin incubation. In conclusion mixing of hybrid leucaena hybrid with mulberry foliage was able to protect protein degradation from mulberry in the rumen.
Supplementation of molasses and branched-chain amino acid to increase in vitro digestibility of ammoniated corn cob in ruminants feed Puastuti, Wisri; Yulistiani, Dwi; Handiwirawan, Eko
Indonesian Journal of Animal and Veterinary Sciences Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.856 KB) | DOI: 10.14334/jitv.v22i4.1664

Abstract

Corn cob contains high fiber and lignin which causes low nutritive value. The objective of the study was to improve the digestibility of ammoniated corn cob (CC) by supplementation of molasses and branched-chain amino acid (BCAA: valine, leucine and isoleucine). CC was processed by addition 3% urea. The first stage of in vitro test was done with 4 levels of molases 0, 5, 10 and 15% of dry matter (DM) of CC. The experiment was carried out using complete randomized design with 4 treatments and 4 replications. The second stage was also in vitro study of supplementation of two levels each for valine = V, leucine = L and isoleucine = I namely 0.1 and 0.2% of DM of ammoniated CC. There were 8 treatments combination of the BCAA as follow: A = V0.1 L0.1 I0.1; E = V0.2 L0.1 I0.1; B = V0.1 L0.1 I0.2; F = V0.2 L0.1 I0.2; C = V0.1 L0.2 I0.1; G = V0.2 L0.2 I0.1; D = V0.1 L0.2 I0.2; H = V0.2 L0.2 I0.2 and 1 control (V0.0 L0.0 I0.0). The experiments were done using completely randomized design with 9 treatments and 3 replications. The results showed that treated CC with urea was able to increase protein content by 78% (increased from 3.34% to 5.95%) while neutral detergent fiber (NDF) decreased by 15.4%, acid detergent fiber (ADF) by 7.9% and lignin 16.7%. Addition of molasses in ammoniated CC increased DM digestibility (P <0.05) by 7.5% (41.9 vs 43.51-46.26%) and NDF by 17.7% (38.41 vs 43.76 – 46.31%). Supplementation of BCAA resulted in the highest DM, OM and NDF digestibility (P <0.05) in the treatment of A, C, D and G. Compare to treament I, the digestibility of DM, OM and NDF in G treatment increased by 31.4%, 27.5% and 36.5%, respectively and produced the highest total population of rumen bacterial of 12.4 x 109 colonies /ml. It can be concluded that the digestibility of ammoniated CC increased by the supplementation of 5% molases and BCAA combination consisted of 0.2% valine, 0.2% leucine and 0.1% isoleucine. Kata kunci: Tongkol jagung, amoniasi, molases, asam amino bercabang.