Safika, Safika
The Faculty of Veterinary Medicine of Syiah Kuala University

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2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA Hajar, Siti; Safika, Safika; Darmawi, Darmawi; Sari, Wenny Novita; Rahmi, Erdiansyah; Syahputra, Yandi; Nurliana, Nurliana; Rinidar, Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.484 KB) | DOI: 10.21157/ijtvbr.v1i2.5944

Abstract

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.
11. The Isolation And Identification Of Bacteria Salmonella Sp On Quail Egg Shell In Traditional Markets Ulee Kareng Banda Aceh Erina, Erina; Azmansyah, Azmansyah; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Safika, Safika; N Siregar, Tongku
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.344 KB) | DOI: 10.21157/j.med.vet..v13i1.4226

Abstract

This research aim was to determine the level of bacterial contamination of Salmonella sp on quail egg. The samples used in this research was 24 shell of quail eggs obtained from eight stores in traditional markets in Ulee Kareung Banda Aceh. The shell was crushed with a mortar and then cultured on SCB media (selenite cysteine broth). Isolation and identification was conducted by using SSA media culture, Gram staining, and biochemical tests. The results of this research showed that the shell of quail eggs contained Salmonella sp. Therefore it can be concluded that the quail eggs sold in Ulee Kareung traditional market were contaminated by Salmonella sp.