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Journal : Biota Jurnal Ilmiah Ilmu-Ilmu Hayati

Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Mursyanti, Exsyupransia; Lestari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.3 KB) | DOI: 10.24002/biota.v10i2.2843

Abstract

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, medium?s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncan?s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 µg/ml) was on 18 hrs of incubation period.
Eksplorasi Genom, terkuaknya misteri manusia (Kajian Buku) Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.342 KB) | DOI: 10.24002/biota.v10i3.2882

Abstract

Eksplorasi genom manusia yang terwadahi dalam Human Genom Project (HGP) telah berhasil mengidentifikasi keseluruhan genom (DNA) manusia dalam waktu 13 tahun (1990-2003), dua tahun lebih cepat dari yang ditargetkan (15 tahun). Karya yang spektakuler ini memberikan banyak informasi tambahan mengenai genom manusia. Genom manusia yang dahulu diperkirakan berukuran tiga milyar basa, ternyata tersusun atas 2,3 milyar nukleotida yang terdiri dari ±30.000 gen, 50% gen tersebut sudah diketahui fungsinya. Kromosom no.1 mengandung gen paling banyak (2.968 gen) sedangkan kromosom Y mengandung gen paling sedikit (231 gen). Selain itu, diinformasikan pula bahwa pembeda manusia yang satu dengan yang lain terletak pada tiga juta lokasi single nucleotide polymorphisms (SNPs).
Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Wahyuningsih, Sisilia Sri; Mursyanti, Exsyupransia; Atmodjo, P. Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.385 KB) | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.