Articles

Found 8 Documents
Search

Micropropagation of Mini Orchid Hybrid Phalaenopsis “Sogo Vivien” Mursyanti, Exsyupransia; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Faculty of Biology, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.637 KB) | DOI: 10.22146/jtbb.12933

Abstract

Phalaenopsis “Sogo Vivien” is an orchid hybrid with mini size plant body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental pot plant. Some plants of this orchid exhibit variegated leaves that improve the beauty of the plant, not only because of the flower but also as attracted leaves. This orchid has high economical value, but mass propagation of this orchid has not established yet. An effective method to propagate both the normal and variegated plants is worth to be generated. The objective of this research was to produce a large number of P. “Sogo Vivien” plants, including the variegated plants. The method used seeds from self pollinating variegated plant, and flower stalk nodes. The seeds were sown on three various medium: VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 + active carbon. The results showed that the best medium for in vitro culture of P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets. Most plantlets emerged from the seeds were non variegated, only one plantlet out of 1344 seeds was variegated (0.007%). Although all emerged plantlets from flower stalk exhibited variegated leaves. Particularly, the plantlets arised from the second and third basal nodes of flower stalk showed the highest growth rate than that from the other nodes. Histological analysis showed that at 11-13 days after shoot segment plantation on NP medium, the shape of apical cells in the nodes was changed, then followed by the change of cell shape in the basal part of the nodes, produced bipolar pattern, then gradually developed into shoot. These results suggest that mass propagation could be achieved using seed culture, but to get the variegated phenotypes, the second and third nodes of flower stalk from variegated plant were the best explants to be used.
Induction of Somatic Embryogenesis through Overexpression of ATRKD4 Genes in Phalaenopsis “Sogo Vivien” Mursyanti, Exsyupransia; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1208.768 KB) | DOI: 10.22146/ijbiotech.15276

Abstract

Phalaenopsis “Sogo Vivien “is a mini orchid hybrid with beautiful flowers and numerous inflorescences.Mass propagation of this orchid is needed to meet the market demand. Objective of this research was toinduce somatic embryogenesis of P.”Sogo Vivien” through insertion of AtRKD4 gene into orchid. T-DNAcontaining 35S::GAL4::AtRKD4::GR was inserted into 16-22 days after sowing orchid protocorms mediated byAgrobacterium tumefaciens EHA 105. Activation of the AtRKD4 gene was induced by glucocorticoid inductionsystem, using 15μM Dexamethasone (Dex). The results showed that 34 out of 2,648 orchid embryos developedinto protocorms on hygromycin selection medium, whereas only 4 out of 2,897 non-transformant protocormsdeveloped from embryos. A 500 bp of HPT genes was amplified from transformant candidates using specificprimers for HPT (HygF1 and HygR1) and 380 bp was amplified using specific primers for AtRKD4 (AtRKD4F1 and AtRKD4 R1), indicated that transgenes have been integrated into orchid genomes. Finally, 17 plantletswere positively carrying AtRKD4 and HPT genes, the efficiency of transformation was 0.63 %. Somatic embryoswere also emerged from leaf explants of transformant on hormone-free NP medium and became normalplantlets. It is probably due to the high activity of AtRKD4 genes in orchids
Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien” Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR Semiarti, Endang; Mursyanti, Exsyupransia; Suyoko, Ahmad; Perdana, Faiza Senja Widya; Widyastuti, Catharina Tri; Subchan, Aditya Nur
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.81 KB) | DOI: 10.14421/biomedich.2018.71.5-13

Abstract

Orchid is an elegant ornamental plant and favoured by the society. Phalaenopsis "Sogo vivien" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. "Sogo vivien" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L-1) and IBA (0, 1, 2 mg.L-1) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L-1 TDZ +1 mg.L-1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%.
Pelatihan Tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) Bagi Guru Biologi SMA Daerah Istimewa Yogyakarta (Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province) Sidharta, Boy Rahardjo; Mursyanti, Exsyupransia; Atmodjo, P. Kianto; To’bungan, Nelsiani; Arsiningtyas, Ines Septi
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 2 (2018): Vol 2, No 2 (2018)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstrakKegiatan Pengabdian kepada Masyarakat dengan topik “Pelatihan tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) bagi Guru Biologi SMA Daerah Istimewa Yogyakarta” dilakukan mengingat banyaknya kasus keracunan akibat konsumsi obat tradisional. Guru Biologi SMA menjadi sasaran pelatihan karena telah memiliki latar belakang keilmuan dan keterampilan yang memadai serta sering menghadapi pertanyaan orangtua murid dan masyarakat perihal pemanfaatan BOT. Pelatihan diharapkan memberikan solusi terhadap permasalahan di atas dengan menerapkan teknologi tepat guna yang sederhana, sehingga dapat memberikan pemahaman tentang BOT berkualitas berdasarkan Cara Pembuatan Obat Tradisional yang Baik (CPOTB). BOT yang dipilih yaitu sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), dan pule (Alstonia scholaris) karena banyak digunakan dalam pengobatan diabetes mellitus. Peserta diberikan keterampilan mengenali, mengidentifikasi, dan melakukan pemeriksaan mutu secara sederhana, namun ilmiah. Peserta menyatakan mampu menerapkan keterampilan yang diperoleh dan bersedia mengikuti pelatihan lanjutan di masa mendatang. Kata kunci: Pengenalan, Pemeriksaan, Penjaminan Mutu, Bahan Obat Tradisional  AbstractCommunity Service Activity with the topic “Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province” was done due to the increase of traditional medicine intoxications. Biology High School teachers were targeted as the participants, because they had scientific background and skills related to the problem. The activity was done to give better solution to the problem using simple and appropriate technology, hence it can give knowledge on high quality of traditional medicine based on Good Production of Traditional Medicine. Traditional medicines utilised were sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), and pule (Alstonia scholaris), since these traditional medicines were mostly practiced to cure diabetes. Participants were also given skills on determination, identification, and quality control. Participants stated that they were able to apply the skills obtained and were ready to be included in the continual training in the future. Key words: Determination, Identification, Quality Control, Traditional Medicine
Toxicity of Bioactive Compound from Endophytic Fungi Isolated from Red Ginger (Zingiber officinale var. rubrum) Utilizing Brine Shrimp Lethality Assay Prasetyo, Angga; Sidharta, Boy Rahardjo; Hartini, Yustina Sri; Mursyanti, Exsyupransia
Biogenesis: Jurnal Ilmiah Biologi Vol 7, No 1 (2019)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i1.6000

Abstract

Red ginger (Zingiber officinale var. rubrum) has been proven to show anticancer activity. Direct use bioactive compound from red ginger has many obstacles such as large amount of red ginger’s rhizome needed, limitation of planting area, and very long time of harvesting. Utilization of endophytic fungi from red ginger’s rhizome could be an alternative to the problems. The aims of this study were to determine bioactive compound produced by endophytic fungi and toxicity activity based on LC50. Endophytic fungi were isolated from red ginger and were identified macroscopically and microscopically. The bioactive compounds were extracted using ethanol 96%. Flavonoid test was done qualitatively, bioactive compounds were analyzed by Thin Layer Chromatography (TLC), and the toxicity test was done using Brine Shrimp Lethality Assay (BSLA). The present research found two endophytic fungi isolated from red ginger rhizome. Isolate 1 was similar to Mucor sp. and isolate 2 was similar to Trichoderma sp. Phytochemical test revealed bioactive compound extracted from the isolates were contained flavonoid. TLC analysis did not detect quercetin from the bioactive compound extracted from the isolates. LC50 values of the bioactive compound from the isolates were 2.300 and 1.747 µg/ml, respectively. The toxicological results suggest that both isolates produce non-toxic compound to Artemia salina.
Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Mursyanti, Exsyupransia; Lestari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.3 KB) | DOI: 10.24002/biota.v10i2.2843

Abstract

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, medium?s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncan?s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 µg/ml) was on 18 hrs of incubation period.
Eksplorasi Genom, terkuaknya misteri manusia (Kajian Buku) Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.342 KB) | DOI: 10.24002/biota.v10i3.2882

Abstract

Eksplorasi genom manusia yang terwadahi dalam Human Genom Project (HGP) telah berhasil mengidentifikasi keseluruhan genom (DNA) manusia dalam waktu 13 tahun (1990-2003), dua tahun lebih cepat dari yang ditargetkan (15 tahun). Karya yang spektakuler ini memberikan banyak informasi tambahan mengenai genom manusia. Genom manusia yang dahulu diperkirakan berukuran tiga milyar basa, ternyata tersusun atas 2,3 milyar nukleotida yang terdiri dari ±30.000 gen, 50% gen tersebut sudah diketahui fungsinya. Kromosom no.1 mengandung gen paling banyak (2.968 gen) sedangkan kromosom Y mengandung gen paling sedikit (231 gen). Selain itu, diinformasikan pula bahwa pembeda manusia yang satu dengan yang lain terletak pada tiga juta lokasi single nucleotide polymorphisms (SNPs).
Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Wahyuningsih, Sisilia Sri; Mursyanti, Exsyupransia; Atmodjo, P. Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.385 KB) | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.