Articles

Found 10 Documents
Search

Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior) Rusanti, Alfindah; Sukandar, Dede; Rudiana, Tarso; Adawiah, Adawiah
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 1, Mei 2017
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v3i1.3640

Abstract

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2?-dihydroxyflavanone, 3-hydroxy-3,4?-dymethoxyflavone and 4?-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640
PEMANFAATAN MINYAK ATSIRI KULIT BUAH HONJE SEBAGAI ANTIOKSIDAN PRODUK SOSIS AYAM Sukandar, Dede; Muawanah, Anna; Rudiana, Tarso; Aryani, Khilda Fithri
Jurnal Teknologi dan Industri Pangan Vol 28, No 1 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2017.28.1.20

Abstract

A study on the inhibition of oxidative deterioriation of chicken sausage products added with essential oil from honje (Etlingera elatior) fruit skin was conducted. The purpose of this study was to determine the antioxidative activity of essential oils of fruit peels of honje and its effect on the inhibition of oxidative deterioration of chicken sausage products. The antioxidant activity assay of the  essential oils and chicken sausages added with 0, 50, 100, and 160 µg/mL essential oil was then analyzed. The best formula of chicken sausages was determined by organoleptic test, i.e.a hedonic test on untrained panelists and by the inhibition of oxidative deterioration measured as malondialdehyde content. In addition, analysis of moisture, ash, fat, protein, carbohydrate, metal contamination and microbial contamination of the sausages was also done. The results showed that the essential oil of the honje fruit skin had  an antioxidant activity (IC50) of 21.296 mg/mL, and the best chicken sausages was those added with  µg/mL essential oils. The characteristics of the best chicken sausages complied with SNI 01-3820-1995 sausage quality standard, with water content of 66.75% (w/w); ash content of 1.26% (w/w); fat content of 2.90% (w/w); protein content of 23.10% (w/w); carbohydrate content of 5.99% (w/w); Metal Zn 14.17 mg/kg; Cu 0.65 mg/kg; and no microbial contamination.
INHIBISI EKSTRAK ETIL ASETAT KULIT BATANG BINTARO (Cerbera odollam Gaertn) TERHADAP PERTUMBUHAN BAKTERI PENYEBAB PLAK GIGI (Streptococcus mutans) Suryani, Nani; Rudiana, Tarso
BIOTIKA Jurnal Ilmiah Biologi Vol 16, No 1 (2018): Biotika Juni 2018
Publisher : Biologi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.524 KB)

Abstract

Plak gigi terbentuk oleh biofilm yang menutupi permukaan gigi. Biofilm merupakan lapisan lendir yang terdiri dari jutaan sel bakteri, saliva dan sisa makanan. Salah satu bakteri utama penyebab plak gigi adalah Streptococcus mutans. Salah satu tumbuhan yang berpotensi memiliki aktivitas antibakteri adalah tumbuhan Bintaro (Cerbera odollam G.), di masyarakat tumbuhan ini hanya digunakan sebagai tumbuhan hias. Belum ada yang penelitian aktivitas antibakteri tumbuhan C. odollam G. terhadap S. mutans, oleh karena itu penelitian ini bertujuan melihat potensi tumbuhan C. odollam G terhadap pertumbuhan bakteri S. Mutans. Tumbuhan C. odollam G. diekstraksi dan dipartisi menggunakan pelarut metanol, n-heksana, dan etil asetat. Berdasarkan hasil pengujian fitokimia dengan reagen spesifik dan KLT, kulit batang C.odollam G. mengandung saponin, tanin, fenolik, flavonoid, alkaloid, steroid dan terpenoid. Berdasarkan hasil pengujian antibakteri terhadap S.mutans, ekstrak etil asetat memberikan inhibisi sebesar 15; 17,5; 23,5 dan 26 mm pada konsentrasi 20, 40, 60 dan 80 %, pada konsentrasi 60 dan 80% diameter inhibisi lebih baik dari klorheksidin. 
Senyawa 5,3'-Dihidroksi-7,4'-Dimetoksiflavon dari Kulit Batang Tanaman Akway (Drimys beccariana Gibs) dan Aktivitas Antimalarianya Rudiana, Tarso; Herlina, Tati; Julaeha, Euis
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 2, November 2018
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v4i2.7775

Abstract

Akway (Drimys beccariana Gibs) is an endemic plant of Papua. Ethnobotany is used as a medicinal plant, one of which is used as a malaria drug. This study aims to isolate, determine the chemical structure and evaluate the antimalarial activity of D. beccariana stem bark. Powder of D. beccariana bark is macerated with methanol and fractionated using n-hexane and ethyl acetate. Ethyl acetate extract was fractionated using gradient liquid chromatography (KCV) (n-hexane: ethyl acetate: methanol). The KCV fraction was separated by repeated gravity column chromatography and preparative thin layer chromatography (TLC-P) to obtain Isolate 1. Isolate 1 was determined by spectroscopic methods including UV-Vis, IR, 1D NMR, and 2D. Isolate 1 is determined by its compound structure which is 5,3'-dihydroxy-7,4'-dimethoxyflavone. 5,3'-dihydroxy-7,4'-dimethoxyflavone compounds evaluated antimalarial activity against Plasmodium falciparum in vitro with antimalarial activity better than artemisinin.
Asam Protokatekuat dari Ekstrak Etil Asetatbiji Honje (Etlingera elatior) dan Uji Aktivitas Antioksidannya Sukandar, Dede; Umedi, Ibnu Umarudin; Nurbayti, Siti; Rudiana, Tarso; Fathoni, Ahmad
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 1, Mei 2018
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v4i1.7225

Abstract

Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas antioksidan dari ekstrak etil asetat biji honje (E. elatior). Isolasi senyawa fenolik dilakukan dengan metode maserasi, fraksinasi dengan kromatografi kolom gravitasi (KKG), dan kromatografi lapis tipis (KLT). Uji aktivitas antioksidan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan penentuan struktur senyawa menggunakan spektroskopi UV-Vis, FTIR, NMR, dan MS. Isolat yang diperoleh berupa gum kuning sebanyak 18 mg dari 3 kg sampel kering. Hasil uji aktivitas antioksidan menunjukkan isolat dari ekstrak etil asetat memiliki aktivitas yang sangat kuat dengan IC50 1,32 µg/mL. Hasil analisis dengan spektroskopi UV-Vis, FTIR, NMR, dan MS menunjukkan isolat sesuai dengan rumus molekul C7H6O4 yang dikenal dengan asam protokatekuat (asam 3,4-dihidroksi benzoat).DOI:http://dx.doi.org/10.15408/jkv.v4i1.7225
Identifikasi Komposisi Kimia dan Uji Aktivitas Antioksidan dari Biji Kurma (Phoenix dactylifera) Siregar, Yusraini Dian Inayati; Rudiana, Tarso; Riyadi, Windi
Jurnal Kimia VALENSI Jurnal Kimia VALENSI Volume 4, No. 2, November 2018
Publisher : Study Program of Chemistry Faculty of Sciences and Technology UIN Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v4i2.8818

Abstract

Dates are a component of dates that have 6.10-11.47% of all dates. In some countries, dates are a major problem in the processing industry because the seeds currently only become waste. This research was conducted to determine the solvent that produces the largest yield using the soxhletation method and determine its antioxidant activity. Dates are disoxletasion with a variety of solvents n-hexane, diethyl ether, and ethanol. Also carried out the variation of extraction time for 1.5; 2; and 2.5 hours. Based on the results of the study showed that the results of the largest date seed extract were obtained using ethanol of 4.93% for 2.5 hours. Antioxidant activity of date palm seed extract (IC50) using DPPH ethanol extract method has IC50 of 2.27 ppm; n-hexane extract 8396.56 ppm; and diethyl ether extract 9826.88 ppm. Inhibition of date palm seed extract using the TBA method derived from ethanol extract of 98.22% at a concentration of 250 ppm. The results of the analysis using LC-MS showed that ethanol extract was thought to contain 13-hydroxyabscisic acid compound, 1-ethylidene-4-methylidene-2oxo-7-(propan-2-il)-tocahydro-1H-indent-5-il- 3-methylpent-2-enoate, 12-hydroxy-acid (8,10,14) -eicosatrienoic and 1-hydroxy-3- (pentadecanoiloxy) propan-2-il (5,8,11,14) -icosa-5, 8,11,14-tetraenoate. The results of the analysis using GC-MS on n-hexane extract showed 2,4-di-tert-butylphenol and 9-octadecenoic acid. Diethyl ether extract contains 9-octadecenoic acid and mono (2-ethylhexyl) benzocarboxylic acid.  
Isolation and Structure Determination of Antioxidants Active Compounds from Ethyl Acetate Extract of Heartwood Namnam (Cynometra cauliflora L.) Sukandar, Dede; Nurbayti, Siti; Rudiana, Tarso; Husna, Tsalats Wahyul
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 19, No 1 (2017)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.193 KB) | DOI: 10.14203/jkti.v19i1.325

Abstract

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring at band I (λmax 330.22 nm), chromophore group of alkene (C=C) at band II (λmax 268.67 nm) and functional groups such as O−H (3343.91 cm-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1), C−O (1269.89) and C−H ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at δH 6.86 ppm (1H, s), C-6 at δH 5.95 ppm (1H, d, J=1.95 Hz), C-8 at δH 6.25 ppm (1H, d, J= 1.95 Hz), C-2’ and C-6’ at δH 7.03 ppm (2H, d, J=7.87 Hz), C-3’ and C-5’ at δH 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC50 value of 5.18 ± 0.014 ppm.
Characterization and antioxidant assay of yellow frangipani flower (Plumeria alba) extract Fathoni, Ahmad; Rudiana, Tarso; Adawiah, Adawiah
JURNAL PENDIDIKAN KIMIA Vol 11, No 1 (2019): April 2019
Publisher : JURNAL PENDIDIKAN KIMIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v11i1.13034

Abstract

Yellow frangipani flowers (Plumeria alba) are plants that commonly found in the tropics, including Indonesia. Frangipani utilization has not optimum and research about it has not many conducted. This study aims to characterize and test the antioxidant activity of frangipani flower extract. The extraction conducted by maceration method using n-hexane, ethyl acetate, and methanol as solvents. Characterization includes phytochemicals assay (alkaloids, flavonoids, terpenoids, phenolic hydroquinone, tannin-polyphenols, and saponins), total phenolic assay, and total flavonoid assay. Antioxidant test using DPPH radical scavenging assay. The results of the characterization showed that frangipani flower extract positively contained alkaloid, flavonoids, tannins compounds in all solvent phases. Terpenoids are positive in n-hexane and ethyl acetate extract, while saponins are positive in methanol extract. The total phenolic content of n-hexane, ethyl acetate, and methanol extract were 3.6 mgGAE/g; 27.74 mgGAE/g; and 35.23 mgGAE/g. The total flavonoid content of n-hexane, ethyl acetate, and methanol extract were respectively 0.8 mgQE/g; 12.18 mgQE/g; 19.9 mgQE/g. The strongest antioxidant activity is possessed by ethyl extract, followed by methanol extract, and the last are n-hexane extract.Keywords:Antioxidant; frangipani flower; phytochemical; total phenolic; total flavonoid
INHIBISI EKSTRAK ETIL ASETAT KULIT BATANG BINTARO (Cerbera odollam Gaertn) TERHADAP PERTUMBUHAN BAKTERI PENYEBAB PLAK GIGI (Streptococcus mutans) Suryani, Nani; Rudiana, Tarso
BIOTIKA Jurnal Ilmiah Biologi Vol 16, No 1 (2018): Biotika Juni 2018
Publisher : Departemen Biologi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.524 KB) | DOI: 10.24198/bjib.v16i1.17476

Abstract

Plak gigi terbentuk oleh biofilm yang menutupi permukaan gigi. Biofilm merupakan lapisan lendir yang terdiri dari jutaan sel bakteri, saliva dan sisa makanan. Salah satu bakteri utama penyebab plak gigi adalah Streptococcus mutans. Salah satu tumbuhan yang berpotensi memiliki aktivitas antibakteri adalah tumbuhan Bintaro (Cerbera odollam G.), di masyarakat tumbuhan ini hanya digunakan sebagai tumbuhan hias. Belum ada yang penelitian aktivitas antibakteri tumbuhan C. odollam G. terhadap S. mutans, oleh karena itu penelitian ini bertujuan melihat potensi tumbuhan C. odollam G terhadap pertumbuhan bakteri S. Mutans. Tumbuhan C. odollam G. diekstraksi dan dipartisi menggunakan pelarut metanol, n-heksana, dan etil asetat. Berdasarkan hasil pengujian fitokimia dengan reagen spesifik dan KLT, kulit batang C.odollam G. mengandung saponin, tanin, fenolik, flavonoid, alkaloid, steroid dan terpenoid. Berdasarkan hasil pengujian antibakteri terhadap S.mutans, ekstrak etil asetat memberikan inhibisi sebesar 15; 17,5; 23,5 dan 26 mm pada konsentrasi 20, 40, 60 dan 80 %, pada konsentrasi 60 dan 80% diameter inhibisi lebih baik dari klorheksidin. 
Effect of Beneng Taro Starch (Xanthosoma undipes K. Koch) Concentration as Disintegrant on Active Ingredient and Microbial Limit Test of Paracetamol 500 mg Tablets Dimas, Indriatmoko Danang; Suryani, Nani; Lestari, Dwi Putri; Rudiana, Tarso
Jurnal Kartika Kimia Vol 2 No 2 (2019): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v2i2.39

Abstract

Tablet dosage form consist of active substances and excipients. Excipients or additives in tablet formulations may be fillers, binders and adhesives, disintegrants, lubricants, antiadherents, adsorbents, flavorings, and coloring agents. Excipients are physical, chemical and pharmacological inert substances which are added to tablet dosage formulations to help it meet the technological process requirements, technical specifications, physical appearance, and official quality requirements (pharmacopoeia). An important excipient for tablet dosage form is the disintegrants. Disintegrants affect the release of active medicinal substances from preparations and can then provide the desired therapeutic effect. One of the disintegrants is starch. Plants that have the potential to have high levels of starch are beneng taro. Xanthosoma undipes K. Koch tubers themselves have a starch content of 15.21%. This study aims to determine the effect of the concentration of taro beneng starch on active ingredient and microbial limit of paracetamol 500 mg tablets. Tablets are made by direct pressing method with variations in the concentration of beneng taro starch as a disintegrant, are 0% (FI), 5% (FII), 10% (FIII) and 15% (IV). The results of the determination of the content showed that all formulations met the requirements of the Pharmacopoeia of Indonesia, FI = 100.27%; FII = 99.95%; FIII = 100.06%; and FIV 99.85%. Microbial limit test showed that the Total Plate Count FI = 20 cfu/g; FII = 35 cfu/g; FIII = 10 cfu/g; FIV = 50 cfu/g. Total Yeast and Mold Count are FI = 110 cfu/g; FII = 100 cfu/g; FIII = 50 cfu/g; FIV = 150 cfu/g. Pathogenic bacterial tests show that all formulas are not contaminated with pathogenic bacteria. Keywords: tablets, Xanthosoma undipes K. Koch, paracetamol, determination, microbes.