Nugraha E. Suyatma, Nugraha E.
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor

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PEMANFAATAN AKTIVITAS ANTIMIKROBA SAPONIN DAUN PEPAYA PADA KEMASAN KELOBOT JAGUNG Wahyuningsih, Sri; Suyatma, Nugraha E.; Kusumaningrum, Harsi D.
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 1 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.903 KB) | DOI: 10.6066/jtip.2016.27.1.68

Abstract

This study aimed to utilize saponins from papaya leaves as antifungal on maize husk packaging. Crude and semi-pure saponins were extracted from papaya leaves using Soxhlet and Ultrasonic-Assisted Extraction (UAE) methods. The total saponin contents were determined using UV-Vis spectrophotometry. Their ability to inhibit Aspergillus niger was tested by macrodillution method. The extracts were also applied to maize husk by soaking it for 5 minutes at concentration of 10, 20 and 25 mg/mL. The parameters observed were physical and mechanical properties (tensile strength and elongation), water vapor transmission rate (WVTR), total plate count (TPC) and mold and yeast counts (MYC) after 25 days of storage at room temperature (±28ºC). Drying of young papaya leaves using cabinet dryer resulted in the highest yield of crude saponin (12.96 ± 0.26%). Total saponin content in crude saponin extract was 115.43 mg/g, while in semi-pure saponin extract was 480.19 mg/g. Crude saponin and semi-pure saponin extract at concentration of 25 mg/mL could inhibit the growth of A. niger for 1.35 and 0.36 log CFU/mL, respectively after 24 hours. The WVTR test indicated that treatment with a concentration of 25 mg/mL of crude saponin extracts showed the lowest transmission. The addition of crude saponin and semi-pure saponin extract to the maize husk at the concentration of 10, 20 and 25 mg/mL was able to inhibit the microbial growth, represented by constant number in TPC and MYC for 25 days storage. This study showed that crude saponin and semi-pure saponin extract could improve the functional properties of maize husk as packaging materials.
PENGARUH PENAMBAHAN LILIN LEBAH DAN NANOPARTIKEL SENG OKSIDA TERHADAP SIFAT FISIK DAN MEKANIS FILM BERBASIS KITOSAN Nandiwilastio, Novan; Muchtadi, Tien R.; Suyatma, Nugraha E.; Yuliani, Sri
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 2 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.056 KB) | DOI: 10.6066/jtip.2019.30.2.119

Abstract

Chitosan-based films showed good gel formation capability and gas permeability (CO2 and O2). However, the high permeability of water vapor limits the application of chitosan as a single coating material. Additionally, single chitosan film was also brittle, so the addition of lipid and metal nanoparticles were chosen to solve this problem. This research aimed to determine the effect of the addition of beeswax emulsion and zinc oxide nanoparticles (ZnO-NPs) on the physical and mechanical properties of chitosan based films. The film was prepared by dissolving ZnO-NPs (0, 1 and 2% by weight of chitosan) into 100 mL of distilled water followed by the addition 1 g of chitosan and or, addition of beeswax emulsion (0 and 5% by the volume of solution). The result showed that the addition of beeswax emulsion ZnO-NPs into chitosan based film significantly decreased WVTR value from 24.03 to 10.38 g/m2 hours and the  tensile strength value from 8.30 to 6.92 Mpa. The addition of beeswax emulsion and ZnO-NPs also changed the physical properties of chitosan-based film significantly; in terms appearance, color and thickness. Mean-while, the film elongation percentage changed significantly in the  range of 30.36 to 50.09 % when ZnO-NPs was added into chitosan-based film solution. Overall, the addition of beeswax and ZnO-NPs showed improvement of the physical and mechanical properties of chitosan-based films so that better packaging of fresh food products can be developed.