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Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Susilowati, Dwi Ningsih; Fauziah, Fani; Pranoto, Eko; Hidayat, Ernin; Setyowati, Mamik; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
Effect of foliar fertilizer application on yield, and yield components of tea (Camellia sinensis Var. Assamica (Mast.) Kitamura) Haq, Muthia Syafika; Rachmiati, Yati; Karyudi, Karyudi
Jurnal Penelitian Teh dan Kina Vol 17, No 2 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i2.46

Abstract

The experiment on the effect of foliar fertilizer (29% N, 10% P2O5, 10% K2O, 3% MgO, 5% SO3, and micro compound B, 0,0075% Cu, 0,026% Fe, 0,032% Mn and 0,023% Zn) concentration on growing tea (Camellia sinensis) and productivity was con­ducted in Research Institute for Tea and Cin­chona Experimental Plantation on the altitude of 1.350 m above sea level and Andisol type soilfrom August to November 2013 with GMB 7 clones. The experiment was arranged in a randomized block design with 3 treatments and 4 replicates. Concentration levels of fertilizer were 1) 0% as control, 2) 2%, and 3) 4%. The results showed that productivity increased significantly, control (465,25 g/10 plantation) compared with the plants were treated with 2% (564,58 g/10 plantation) and 4% (573,46 g/10 plantation) fertilizer, respectively. The increase of productivity was due to increased of plucking point, quantity and weight of pecco and length of leaves. 
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fauziah, Fani; Setiawati, Mieke Rochimi; Susilowati, Dwi Ningsih; Pranoto, Eko; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.
The effect of NPK compound fertilizer (27%:6%:10%) compared to conventional fertilizers on productive tea plants of GMB 7 clone in the Andisols soil type Rahardjo, Pudjo; Rachmiati, Yati; Salim, Arkat Agus
Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v15i2.22

Abstract

The effectiveness application of compound fertilizer NPK (27%:6%:10%) to increase  tea shoots had been tested in Experimental Garden of Research Institute for Tea and Cinchona Gambung, Bandung, West Java, from January 2006 to March 2007. Experi­mental design used was randomized block design with 6 treatments with five replication. The plot sizes were 20 x 20 m = 400 m2/plot. The tea clone tes­ted was GMB 7. The appli­cation of compound fertilizer NPK (27%:6%:10%) were twice a year (50%-50%) and conventional fertlization were 4 times a year (20%-30%-20%-30%). The composition of  treatment was as follows: (A) 500 kg, (B) 600 kg, (C) 700 kg, (D) 800 kg/ha/year, and (E) conventional fertilizer recommended dosage of the Research Institute  for Tea and Cinchona Gambung (480 kg urea + 357 kg ZA + 260 kg SP 36 + 300 kg KCl + 278 kg  kieserit/ha/year), and (F) control (no fertilizer treatment). Parameters  observed were: 1. soil and leaf analysis at before and after treatment, 2. weighting the potential and the yield components each time of plucking, and 3. technoeconomic analysis. The results showed that: statistical analysis of the components as well as potential yield, and nutrient analysis (N, P, K, Mg and Zn) of the mother leaves of GMB 7 clones and the soil demon­strated the significantly differences among the treatments of all the various doses of compound fertilizer NPK and conventional fertilizer application to control (no fertilizer treatment). Meanwhile, there were no significantly differences among the result of  the doses of compound fertilizer NPK, to the conventional fertilizer applications. Further­more, dose of compound fertilizer NPK (27%:6%:10%) for produc­tive tea plant was recommendated at 700 kg/ha rate and was equivalent to 189 kg N/ha/year or 63% of the dose of conventional fertilizer, but could provide equivalent result to conventional fertilizer application, and not only could save the buying cost of conventional fertilizer  (urea, ZA, TSP/SP-36, KCl / MOP and kieserit) but also the cost of application cost in the field.
PENGARUH APLIKASI PUPUK DAUN MINERAL DAN ORGANIK CAIR TERHADAP PENINGKATAN PERTUMBUHAN BENIH TEH SIAP SALUR Wulansari, Restu; Rachmiati, Yati; Rezamela, Erdiansyah
Creative Research Journal Vol 2, No 02 (2016)
Publisher : Badan Penelitian dan Pengembangan Daerah (BP2D) Provinsi Jawa Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Persemaian teh merupakan tahapan penting untuk mendapatkan benih teh berkualitas. Permasalahan yang pada penyediaan benih berkualitas adalah persentase benih siap salur rendah. Penelitian ini bertujuan untuk meningkatkan persentase benih siap salur melalui pemberian pupuk mineral dan pupuk organik di persemaian teh. Penelitian dilaksanakan di Persemaian Pusat Penelitian Teh dan Kina, Bandung, Jawa Barat Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok, dengan empat perlakuan dan enam ulangan. Benih yang digunakan adalah klon GMB 7 berumur bibit 8,5 bulan. Perlakuan terdiri dari: pupuk mineral berupa larutan urea 2%, serta 2,5 mL/L, 5 mL/L dan 7,5 mL/L pupuk organik. Hasil penelitian menunjukkan bahwa pertumbuhan bibit teh yang diberi pupuk mineral dengan nyata lebih cepat dibandingkan dengan pupuk organik. Persentase bibit siap salur berumur 13,5 bulan tinggi pada pembibitan dengan pupuk mineral urea, yaitu 53,26%. Penelitian ini menjelaskan bahwa penggunaan larutan urea di persemaian teh memberikan pertumbuhan benih siap salur lebih cepat.