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Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Susilowati, Dwi Ningsih; Fauziah, Fani; Pranoto, Eko; Hidayat, Ernin; Setyowati, Mamik; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.744 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
The effect of foliar fertilizer and pesticide methomyl in tea plantation after infested by pest: (2) The effect on population and attack intensity of Empoasca flavescens Fauziah, Fani; Haq, Muthia Syafika; Karyudi, Dr.; Rosyadi, Achmad Imron
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.843 KB) | DOI: 10.22302/pptk.jur.jptk.v18i2.75

Abstract

The aim of this research was to obtain the effect of insecticide combination with nitrogen and zinc foliar fertilizers to Empoasca flave-scens. An experiment was conducted at Gam-bung Experimental Garden, Bandung, West Java. The trial was arranged in a randomized complete block design with nine treatments (A)methomyl; (B) methomyl + ZnSO4 1%; (C) me-thomyl + ZnSO4 2%; (D) methomyl + N 1%; (E) methomyl + N 1% + ZnSO4 1%; (F) metho-myl + N 1% + ZnSO4 2%; (G) methomyl + N 2%; (H) methomyl + N 2% + ZnSO4 1%; and (I) methomyl + N 2% + ZnSO4 2%, replicated three times. Each unit consisted of experiments is 40 plants. The treatments comprised: concen-tration of methomyl 0,5 gram/l with sprayed volume 200 l/ha. The level concentrations of nitrogen and zinc were 1% and 2%. The appli-cation of treatments was sprayed six times after plucking with 12 days interval, respectively. The population and intensity of E. flavescens attack were measured at the time of plucking. The effectiveness of treatment on the population as well as attack intensity of Empoasca con-ducted by measuring the rate of decline through a linear regression approach. The results showed that all of the treatments could reduce the population as well as the attack intensity of E. flavescens but there were no significantly different among others and were comparable to the chemical insecticide treatment. The fastest rate of decline were indicated by the treatment methomyl + N 2% + ZnSO4 1% and methomyl + N 2% + ZnSO4 2%.
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fauziah, Fani; Setiawati, Mieke Rochimi; Susilowati, Dwi Ningsih; Pranoto, Eko; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.257 KB) | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.
The effect of drought period on attack intensity of Empoasca sp and blister blight in Gambung tea plantation Rezamela, Erdiansyah; Fauziah, Fani; Dalimoenthe, Salwa Lubnan
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.812 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.111

Abstract

El-Nino had a significant impact on micro climate change including in rainfall, temperature and air humidity in Gambung Tea Plantation. In the dry season 2015, Gambung experienced four dry months that affect attack intensity of Empoasca and disease intensity of Blister Blight. This study aimed to determine the relationship between micro climate parameters and attack intensity of Empoasca and disease intensity of Blister Bright. Micro climate observation data were taken from Davis Automatic Weather Station (AWS) including rainfall intensity (mm), relative humidity (%) and maxium air temperature (0C). Attack intensity of Empoasca sp and Blister Bligh were observed by counting the number of healthy P+3 shoots as well as infected shoot from 200-500 gram of wet shoot sample randomly taken from container net at the plucking time. The results showed that the intensity of Empoasca decreased from 16.49% to 12.90% in the dry months from July to October 2015. The disease intensity of Blister Blight only occurred in July. There was a polynomial relationship among rainfall, temperature and humidity to the attack intensity of Empoasca with R2 values of 0.71, 0.77, 0.87, respectively the decrease of attack intensity occurred at ranfall intensity >140 mm, temperature >28°C and humidity >80%. Meanwhile linear relationship occurred among rainfall, temperature and humidity to the disease intensity of Blister Blight with R2 values of 0,98; 0,64; and 0,77, respectively the increasing of rainfall and humidity caused the increasing of disease intensity of Blister Blight. However disease intensity of Blister Blight decreased with increase in ambient temperature.
Foliar Fertilizer and Pesticide Methomyl 25% in Tea Plantation after Infested by Pest. (1) Effect of Productivity Increasing and Yield Components haq, muthia syafika; Fauziah, Fani; Karyudi, Karyudi
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.918 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.65

Abstract

The experiment of effect of Foliar Fertilizer (N& ZnSo4) and pesticide compatibility with Methomyl 25% compound to increasing Tea Productivity (Camellia sinensis) has been conducted in Research Institute for Tea and Chincona experimental plantation1350 m above sea level and andysol type soil. The experiment started from November 2013 – Februari 2014  in produced tea area with 1.080 plant population. Material plantswere GMB 4 clones with second pruning year and 12 days plucking interval. The experiment was arranged on randomized block design with 9 treatments and 3 replicate. Foliar fertilizer and pesticide combination which has been examined  were  (1) Methomyl 25% (control), (2) Methomyl 25% + Zn 1%, (3) Methomyl 25% + Zn 2%, (4) Methomyl 25% + N 1%, (5) Methomyl 25% + N 2%, (6) Methomyl 25% + N 1% + Zn 1%, (7) Methomyl 25% + N 1% + Zn 2%, (8) Methomyl25% + N 2% + Zn 1%and (9) Methomyl25% + N 2% + Zn 2. Spraying application done every after plucking. The results  showed that skiffing treatment can control the tea plantation that infested by pest and stimulate the new bud growth. The results  also showed that productivity increased significantly up to37, 48 % (2.781 gr/40 plants) when the plants were treated with Methomyl + N 1% + Zn 2%.But,the increased production is accompanied by an increase of banjhi because plant still using their nutrition to recovering after pest infested. The weight of pecco which grown from the  lateral buds is relatively small because of skiffing. Bud growth rate after given treatment was obtained only 3-4 cm/ 6 days caused by lack of light intensity at the time of reserached
Pengaruh Kombinasi Aplikasi Jamur Paecilomyces fumosoroseus, Lem Perangkap, dan Insektisida Imidakloprid terhadap Empoasca sp. Fauziah, Fani; Sucherman, Odih
Jurnal Agro Vol 4, No 1 (2017)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.815 KB) | DOI: 10.15575/1229

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In order to obtain an effective and efficient control method in integrated pest management, a research to know the effectiveness of combination treatments on Empoasca sp. on tea plant had been conducted. The trial was carried out at Gambung Experimental Station (1,250 masl) and designed in Randomized Complete Block design (RCB), with 8 treatments and 3 replications. The treatment tested comprised with A. Paecilomyces fumosoroseus (Pfr); B. sticky trap (ST); C. imidacloprid (I); D. Pfr + ST; E. Pfr + I; F. ST + I; G. Pfr + ST + I; and H. control. Pfr multiplied in rice medium 2.5 kg ha-1 and imidacloprid 0.125 L ha-1 were sprayed, while sticky trap was set in the middle of 5 m x 5 m plot. The parameters observed were attack intensity of Empoasca sp., population of Empoasca sp, and tea shoot production. The results showed that after 3 times application of all treatments revealed a decrease in the attack intensity of Empoasca sp. After the fourth application, combination treatment of sticky trap (ST) + imidacloprid (I) consistently showed higher efficacy than other treatments. In the sixth observation, the average of Empoasca sp. attack intensity of combination treatment (ST + I) was 15.95% with an average population of 0.67 Empoasca sp. per plot. In the meantime, tea shoot production was 33.64% higher compared with control.
Kompatibilitas Jamur Entomopatogenik Paecilomyces fumosoroseus dengan Beberapa Bahan Aktif Pestisida Secara In Vitro Fauziah, Fani; Rohdiana, Dadan
Jurnal Agro Vol 3, No 2 (2016)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.106 KB) | DOI: 10.15575/801

Abstract

Untuk mengurangi penggunaan pestisida di perkebunan teh, maka salah satu upaya pengendalian hama yang dapat dilakukan adalah mengkombinasikan aplikasi pestisida dengan jamur entomopatogenik. Penelitian ini bertujuan untuk mengetahui kompatibilitas antara jamur P. fumosoroseus  dengan beberapa bahan aktif pestisida di laboratorium. Perlakuan dirancang dengan Rancangan Acak Lengkap (RAL) terdiri dari 5 jenis bahan aktif yaitu metomil, bifentrin, imidakloprid, tembaga oksida dan metidation pada taraf konsentrasi sesuai rekomendasi lapang (RL), 0,5x RL dan 2x RL. Hasil pengamatan menunjukkan bahwa diantara kelima jenis bahan aktif pestisida yang diuji, metidation memiliki tingkat toksisitas yang paling tinggi. Persentase produksi spora tertinggi sebesar 13,77% ditunjukkan oleh perlakuan bifentrin 2x RL. Combining application of pesticides and entomopathogenic fungi is an alternative way for controlling pest in order to reduce pesticides application in the tea field. This research was aimed toexamine the compatibility of Paecilomyces fumosoroseus tosome active agents of pesticide in laboratory. Completely Randomized Block Designed (CRBD) with 5 active agents: methomyl, bifenthrin, imidacloprid, copper oxide and metidation in three different concentration was employed in this study, which was based on the existing field recommendation (FR): 0,5x FR and 2x FR. The results showed that among the five tested pesticides, metidation appeared to be the most toxic agentto P. fumosoroseus. Meanwhile, the highest percentage of spore production was counted in bifenthrin 2x RL (13,77%.)
PENGARUH APLIKASI INSEKTISIDA BERBAHAN AKTIF ASETAMIPRID TERHADAP ULAT PENGGULUNG PUCUK (Cydia leucostoma) PADA TANAMAN TEH Fauziah, Fani; Maulana, Hilman
JURNAL AGROTEKNOLOGI Vol 8, No 1 (2017): Agustus 2017
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

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Abstract

Tea Shoot roller (Cydia leucostoma Meyrick) is one potential pests that attack tea shoot, mainly on the new tea shoot after prunning, it caused plant growth becomes stunted. One of control that can be done is by application of insecticides. To determine the effectiveness of insecticide Acetamiprid, the trial has been conducted at Block A7, Gambung field trial, Kabupaten Bandung, during ± 3 months, from October up to December 2016. The trial was designed in Randomized Complete Block Design (RCBD) with five treatments and four replications. The treatment tested covered insecticide Asetamiprid 30% at 1; 0.75; 0.50; 0.25 l/ha and control. The insecticide was sprayed six times using knapsack sprayer one day after plucking, with one week interval. Tea shoot roller attack intensity was observed weekly at the time of plucking. Shoot production, phytotoxicity, as well as rainfall were also observed as a supporting data. The results showed that after three times of spraying, insecticide Asetamiprid 30% at all formulation doses tested could effectively suppress the attack intensity of tea shoot roller. The last observation result, after six times spraying, revealed that the average efficacy level relatively high, i.e. 73,24%. Therefore, for controlling tea shoot roller on tea it could be recommended the use of insecticide Asetamiprid 30%.
Pengaruh Pemberian Pupuk Mikro Zn dan Cu serta Pupuk Tanah terhadap Perkembangan Empoasca sp. pada Areal Tanaman Teh Fauziah, Fani; Wulansari, Restu; Rezamela, Erdiansyah
Agrikultura Vol 29, No 1 (2018)
Publisher : Fakultas Pertanian Universitas Padjadjaran

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Abstract

ABSTRACTThe effect of Zn and Cu micro-fertilizer and soil fertilizer on the development of Empoasca sp. in tea plantation areaFertilization has an important role for the entire process of metabolism and improves plant health. Tea plants are plants that require macro and micro nutrient inputs. The micro elements of Zn and Cu are needed for energy production, protein synthesis, formation of auxin hormones and increament of plant resistance to pest attack. This study aimed to determine the effect of Zn and Cu micro fertilizer and soil fertilizer on the development of Empoasca sp. The study was conducted from January to July 2017 at Gambung Experimental Station with an altitude of 1350 asl. The experiment was designed with Randomized Block Design (RBD) Factorial pattern with the following treatment arrangement: Factor A = 1) Without Micro Fertilizer, 2) Micro Fertilizer of Zn and Cu 200 g/ha/application, and 3) Zn and Cu 300 g/ha/application; Factor B = 1) Soil fertilizer of 100% recommended dosage, 2) Soil fertilizer of 75% recommended dosage, and 3) Soil fertilizer of 50% recommended dosage. All treatmnets were repeated three times. Application of micro fertilizers was carried out after six times of tea-leaf picking, while soil fertilizer was applied once at the beginning of the experiment. Observation parameters were population and attack intensity of Empoasca sp., as well as shoot production (kg/25 m2 plot size). The results showed that the application of fertilizers had an effect on the decrease of attack intensity and the population of Empoasca sp. in all treatments. Average attack intensity and population of Empoasca sp. at the end of the observation did not differ significantly of 31.64% and 2.55 Empoasca sp. per plant. Meanwhile, the highest total production of 36.56 kg/plot was obtained from the application of micro fertilizer Zn and Cu in the dosage of 200 g/ha/application at all doses of soil fertilizer, but there was no interaction between treatment factors.Keywords: Micro fertilizer, Soil fertilizer, Empoasca sp., Tea plantABSTRAKPemupukan memegang peranan penting untuk seluruh proses metabolisme dan meningkatkan kesehatan tanaman. Tanaman teh merupakan tanaman yang memerlukan input unsur hara makro dan mikro. Unsur mikro Zn dan Cu diperlukan tanaman untuk produksi energi, sintesis protein, pembentukan hormon auksin serta meningkatkan resistensi terhadap serangan organisme pangganggu tanaman. Penelitian ini bertujuan untuk mengetahui dampak pemberian pupuk mikro Zn dan Cu serta pupuk tanah terhadap perkembangan Empoasca sp. Penelitian dilaksanakan dari bulan Januari hingga Juli 2017 di Kebun Percobaan Gambung dengan ketinggian 1.350 mdpl. Percobaan dirancang dengan Rancangan Acak Kelompok (RAK) pola Faktorial dengan susunan perlakuan sebagai berikut: Faktor A = 1) Tanpa Pupuk mikro, 2) Pupuk mikro Zn dan Cu 200 g/ha/aplikasi, dan 3) Pupuk mikro Zn dan Cu 300 g/ha/aplikasi; Faktor B = 1) Pupuk tanah 100% dosis anjuran, 2) Pupuk tanah 75% dosis anjuran, dan 3) Pupuk tanah 50% dosis anjuran. Perlakuan diulang sebanyak tiga kali ulangan. Aplikasi pupuk mikro dilaksanakan setelah pemetikan sebanyak enam kali, sedangkan pemberian pupuk tanah dilakukan satu kali diawal percobaan. Parameter pengamatan yaitu populasi, intensitas serangan Empoasca sp., dan produksi pucuk (kg/plot 25 m2). Hasil penelitian menunjukkan bahwa pemberian pupuk berpengaruh terhadap penurunan intensitas serangan dan populasi Empoasca sp. pada seluruh perlakuan. Rata-rata intensitas serangan dan populasi Empoasca sp. pada akhir pengamatan tidak berbeda nyata sebesar 31,64% dan 2,55 ekor per tanaman. Sementara itu, total produksi tertinggi diperoleh dari aplikasi pupuk mikro Zn dan Cu dosis 200 g/ha/aplikasi pada semua dosis pupuk tanah yaitu sebesar 36,56 kg/plot, namun tidak terjadi interaksi antar faktor perlakuan.Kata Kunci: Pupuk mikro, Pupuk tanah, Empoasca sp., Tanaman teh
ALTERNATIF PENGENDALIAN Empoasca sp. DENGAN INSEKTISIDA BUPROFEZIN PADA TANAMAN TEH Fauziah, Fani
Creative Research Journal Vol 4, No 02 (2018)
Publisher : Badan Penelitian dan Pengembangan Daerah (BP2D) Provinsi Jawa Barat

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Abstract

Empoasca merupakan hama utama pada tanaman teh yang dapat menimbulkan kerusakan hingga 50%. Penelitian ini bertujuan untuk mengetahui alternatif pengendalian Empoasca dengan insektisida berbahan aktif Buprozein. Penelitian dilaksanakan di Kebun Percobaan Gambung, Kabupaten Bandung, selama ± 3 bulan, dari  bulan Maret hingga Mei. Pengujian dirancang dalam Rancangan Acak Kelompok (RAK) dengan lima perlakuan dan lima ulangan. Perlakuan  yang diuji meliputi insektisida Buprofezin 100 g/l  dengan dosis 0,125; 0,25; 0,375; 0,5 l/ha, dan kontrol. Insektisida disemprotkan dengan menggunakan alat semprot punggung sehari setelah pemetikan, sebanyak 6 kali, dengan interval aplikasi 1 minggu. Parameter yang diamati meliputi intensitas serangan, populasi Empoasca sp., produksi pucuk, suhu dan curah hujan. Hasil pengujian menunjukkan bahwa insektisida Buprofezin 100 g/l  pada semua dosis yang diuji berpengaruh terhadap intensitas dan populasi Empoasca sp. Hasil pengamatan menunjukkan bahwa dosis efektivitas tertinggi ditunjukkan oleh dosis formulasi 0,375; 0,5 l/ha dengan tingkat efektivitas lebih dari 70%.