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Correlations between morphological traits and catechin content on tea (Camellia sinensis (L.) O. Kuntze)

Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Catechin content in tea plant is a major character in tea breeding program for a healthy beverage. A tea breeding program for high catechin content maybe will performed by indirect selecting a trait or traits that signi­ficantly correlated with high catechin content. This research was performed to identify mor­phological traits which maybe significantly correlated with high catechin content in tea plant. A randomized complete block design with three replications was used in this experiment using 10 selected clones. The experiment was performed in Gambung Experimental Station ot the Research Institute for Tea and Cinchona, Gambung, West Java. Catechin content, tri­choma density of young leaves, weight of p+2, size of mother leaves, chlorophyll content of mother leaf, stomatal density of mother leaf, mother leaf thickness, and mother leaf angle, were measured. The results indicated that stomatal density and mother leaf angle were significantly correlated. Based on path analysis indirect selection for high catechin content could be performed by selecting stomatal density considering to trichoma density, mother leaf angle and chlorophyll content. In addition, indirect selection for high catechin content could be performed by selecting other leaf angle independently without considering other morphological traits.

The growth test of tea clones (Camellia sinensis) from cross pollination first generation clones

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Efforts to improve the productivity of tea needed new high yielding clones were obtained from the cross and easily propagated vegetatively. Preliminary testing of the clones  needs to be done to determine the growth of cuttings and rooting properties of tea clones from hybridization which are selected by potential production. This study used a Randomized Complate Block Design with three replications. The treatments consist of 14 clones from crosses and GMB 7 as clone comparison. The data is analyzed by using ANOVA and Scott Knott test with the level of 5%. The observations are intended to look at the growth of roots and shoots by dismantling seedling in polybag predetermined randomly at each observation. The results showed that the clones tested had good growth with a low mortality rate, and easily propagated vegetatively. The S / R ratio also showed shoot growth more faster than the growth of root. This character is advantageous because the most appropriate selection criteria for planting in the field.

Vegetative growth ability of artificial cross pollinated of tea clones

Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Vegetative propagation is common system that carried out in tea propagation. Seedling propagation was not reliable due to time consuming and phenotypic variability in seed plant. Despite yield potential, good quality, pest and disease resistant characters, the easy of vegetative propagation is very important character in tea breeding process. The aim of this study is to selected clones that resulted by artificial pollination for an ability of vegetative propagation character. Plant materials that tested in this experiment were the seed from the vegetative propagation of 14 clones, and GMB 7 clone as a control. Randomized Block Design was used in this experiment by 3 replications. The data analysis was ANOVA and Scott Knott test. The result of the experiment showed that clone number 41, 45, 38, 149, X5, X3, 122, 78, and 44, were the easiest clones to be vegetative propagated.

Identification of catechin content and its derivates of tea germplasm collection clones

Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Katekin merupakan salah satu poliphenol utama pada tanaman teh yang menjadikan teh sebagai minuman yang baik untuk dikonsumsi. Program pemuliaan tanaman teh dalam merakit klon yang memiliki kandungan katekin tinggi tidak dapat dilepaskan dari plasma nutfah teh. Informasi akan kandungan katekin klon koleksi plasma nutfah dapat menjadi pegangan bagi pemulia dalam melakukan seleksi tetua untuk proses persilangan buatan. Selain itu klon – klon koleksi plasma nutfah dengan kandungan katekin tinggi dapat dikembangkan langsung sebagai klon anjuran yang dapat meningkatkan kandungan katekin bagi para pekebun. Penelitian ini bertujuan untuk mengetahui kandungan katekin pada klon koleksi plasma nutfah teh. Percobaan dilakukan di Kebun Percobaan Gambung Pusat Penelitian Teh dan Kina, Kabupaten Bandung, Jawa Barat. Ketinggian tempat 1300 mdpl, dengan jenis tanah Andisol. Analisis kandungan katekin dilakukan di laboratorium dengan menggunakan metode yang tertera pada AOAC (1984). Bahan tanaman yang digunakan merupakan klon teh generasi pertama koleksi plasma nutfah sebanyak 10 klon, yaitu klon: Cin 20, Cin 29, Cin 149, RS 1, RB 1, RB 2, PS 354, PS 385, KP 2, SA 73. Karakter yang diamati dalam percobaan ini adalah kandungan katekin total, EGC, ECG dan EGCG. Analisis data yang digunakan adalah analisis keragaman dan uji lanjut menggunakan uji gerombol Scott-Knott. Hasil dari penelitian ini adalah (1) Kandungan katekin total dan derivatnya daiantara klon koleksi aksesi plasma nutfah berbeda-beda; (2) Klon dengan kandungan katekin total tertinggi adalah klon RB 1 dan terendah adalah  klon RS 1dan Cin 149; (3) Klon dengan kandungan EGCG yang tertinggi adalah klon RB 1 dan RB 2 dan terendah adalah klon Cin 149 dan RS 1. (4) Klon dengan kandungan ECG tertinggi terdapat pada klon Cin 149 dan terendah pada klon PS 354 dan Cin 20.