Amy Nindia Carabelly, Amy Nindia
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THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Carabelly, Amy Nindia; Taat Putra, Suhartono; Suardita, Ketut
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

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Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
Ekstrak metanol batang pisang mauli (Musa sp.) dosis 125-1000 mg/kg bb tidak menimbulkan efek toksik pada hati mencit (Mus musculus) Mauli methanol extract of banana (Musa sp.) dosages of 125-1000 mg/kg bw is not cause toxic effects on the liver of mice (Mus musculus) Apriasari, Maharani Laillyza; Carabelly, Amy Nindia; Andini, Ghea Tri
Journal of Dentomaxillofacial Science Vol 12, No 2 (2013): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v12i2.355

Abstract

Musa sp. is one of Kalimantan Selatan original plant that empirically has benefit as herbal medicine for acceleration of wound healing. Banana contain many phytochemicals such us saponin, flavonoid, and tannin which can be used asantibiotic, pain relieving and anti inflamantory. This experiment was aimed to know the toxicity effect of methanolicextract of mauli banana stem inmice livers histopathological. The research is pure experimental study using post testonly with control design. Populations were 25 mices, divided into 5 groups. Group I as negative control group weregiven aquadest, and group II, III, IV, and V were given 125 mg, 250 mg, 500 mg, and 1000 mg methanolic extract ofMauli banana stem. They were given treatment daily as long as 21 days. All of the mice were necropsied at day 22, andthe livers were taken for to examine their histopatological changes. Each liver tissue was processed by paraffin blockembededdand hematoxylin eosin staining method. Histopathological changes were examined based on the present necrosis, degeneration, and megalohepathosit lesions. The results showed light necrosis, degeneration, andmegalohepathosit on the treatment group. The conclusion was methanolic extract of Mauli banana stem dosage 1251000mg/kgbwis not toxic significantly on the liver of mice.
Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell) Apriasari, Maharani Laillyza; Carabelly, Amy Nindia; Aprilia, Gusti Febby
Journal of Dentomaxillofacial Science Vol 13, No 1 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i1.384

Abstract

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.
Angular cheilitis and oral pigmentation as early detection of Peutz-Jeghers syndrome Apriasari, Maharani Laillyza; Carabelly, Amy Nindia
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 1 (2018): (March 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.174 KB) | DOI: 10.20473/j.djmkg.v51.i1.p29-32

Abstract

Background: Peutz-Jeghers syndrome (PJS) is an inherited autosomal dominant disease determined by a mutation localized at 19p13.3 characterized by the occurrence of gastrointestinal hamartomatous polyps in association with mucocutaneous hyperpigmentation. The manifestation of PJS may first be encountered by a dentist during routine examination due to the presence of pigmented spots in the oral cavity. Purpose: To prevent a high risk of PJS, the dentist must establish its oral manifestation through early detection. Case: A 14-year-old male patient attended complaining of a week-long pain at the corners of the lips. An extra-oral exam revealed fissure lesions, redness, white crust and pain. The patient had experienced bleeding in his bowel movements, abdominal pain, nausea and vomiting since childhood. A number of black, painless, macular lesions, some 1-3 mm in diameter, were present on the upper lips, lower lips, fingers and palms. Case management: The patient was referred for a complete blood count check. The results obtained confirmed him to be suffering from severe anemia and he was, therefore, referred to an internist for treatment for PJS. Conclusion: It can be concluded that the early detection of PJS is crucial in order that the patient receives prompt treatment.
THE EFFECT OF GIANT SNAKEHEAD(Channa micropeltes)EXTRACT ON THE NUMBER OF MACROPHAGE IN DIABETES MELITUS WOUND HEALING (Research report) Khairunnida, Khairunnida; Carabelly, Amy Nindia; Apriasari, Maharani Lailyza
Dentino Vol 4, No 1 (2019)
Publisher : FKG Unlam

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Background:Giant snakehead contains albumin and omega-6 fatty acids which play an important role in diabetes mellitus wound healing. Wounds in people with diabetes mellitus have low level of albumin, thus inhibiting the macrophage function. Objective: To prove the effect of giant snakehead extract of 16mL/Kg dose of rats’ weight toward the number of macrophage on wistar rats’ back wounds with diabetes mellitus in day 4, 8 and 14. Method and Materials: This study applied pure experimental method with posttest-only control design. This study 36 wistar rats as sample which divided into 9 groups; group of giant snakehead extract with 16mL/Kg dose of rats’ weight, positive control group using haruan fish extract with 13.54mL/Kg dose of rats’ weight, and negative control group using BR2 feed. Results: The test result of One-way ANOVAon day 4 (p=0.022), day 8 (p=0.251) and day 14 (0.028). The test result of Post-hoc LSD showedthat there wereno significant differences on day 4 and 14 (p>00.5) between the groups of the giant snakehead extract and haruan fish extract. There wassignificant difference (p<00.5) between groups of the giant snakehead extract and BR2 feed. Conclusion: It can be concluded that giant snakehead extract at 16 mL / kg dose administered orally may increase the number of macrophage in wistar rat (Rattusnovergicus) induced diabetes mellitus back woundon day 4 and decreased macrophage cell count onday 8 and day 14.
TOXICITY TEST OF THE MIXED MOUTHWASH OF MAULI BANANA STEM AND BASIL LEAF AGAINST FIBROBLAST CELL STUDY IN VITRO Saputri, Martha Vania; Carabelly, Amy Nindia; Firdaus, I Wayan Arya K.
Dentino Vol 4, No 2 (2019)
Publisher : FKG Unlam

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Abstract

Background: Mauli banana stem and basil leaf are herbal plants which can be used as an herbal mouthwash. People in South Kalimantan often use mauli banana stem to accelerate wound healing because of its antibacterial activity. Basil leaf are proven to inhibit bacterial growth. Mauli banana stem has a bitter taste, so to minimize that bitter taste, basil leaf can be used as a mixture. Basil leaf give fresh and aromatic effect. Extract of mauli banana stem is not toxic against fibroblast cell BHK-21 with 25% concentrate, but there is no research about the toxicity of a mixed mouthwash of mauli banana stem and basil leaf against fibroblast cell BHK-21 yet. Purpose: To analyze IC50 after treatment with a mixed mouthwash of mauli banana stem and basil leaf into the fibroblast cell BHK-21. Method: True experimental using the posttest only with control group design which consisted of 10 treatment groups with several concentrations. Those were 31,25 ?g/mL, 62,5 ?g/mL, 125 ?g/mL, 250 ?g/mL, 500 ?g/mL, 1000 ?g/mL, 2000 ?g/mL and 4000 ?g/mL. The toxicity test used MTT Assay as a method. This research was formed by 2 stages, which were preparation of fibroblast cell BHK-21 and the toxicity test. The cell viability counted with Freshney formula. Result: The result showed that the IC50 was 2183,43 ?g/mL. Conclusion: The mixed mouthwash of mauli banana stem and basil leaf is not toxic against fibroblast cell BHK-21 because IC50 is 2183,43 ?g/mL.
THE EFFECT OF TOPICAL TOMAN (Channa micropeltes) FISH EXTRACT ON THE NUMBER OF NEUTROFIL CELLS IN DIABETES MELITUS WOUND HEALING (In Vivo Study on Male Wistar (Rattus novergicus) Rat’s Back) Nurdianty, Annisa Vara; Apriasari, Maharani Laillyza; Carabelly, Amy Nindia
Dentino Vol 4, No 2 (2019)
Publisher : FKG Unlam

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Backgrounds: Diabetes mellitus forges the wound healing process to last longer. Toman fish which contains albumin and omega-6 fatty acid is proven to to enhance wound healing process in this systemic condition. Patients who suffer from diabetes mellitus retain high neutrophil number. Omega-6 fatty acids contained in Toman fish can decrease the number of  neutrophil cells. Objectives:  To prove the effect of Toman fish (Channa micropeltes) extract at 20% concentration topically on the number of neutrophil in diabetes mellitus-induced Wistar Rat (Rattus novergicus) injuries on day 4, 8 and 14.  Methods: This research was a true experimental study with posttest-only and control group with complete random design. Thirty six male Wistar rats (Rattus novergicus) was divided evenly for three treatments comprised of negative control given BR2 comfeed, positive control treated with Haruan fish (Channa striata) extraxt at 10% concentration topically and treatment group given with Toman fish (Channa micropeltes) extract at 20% concentration topically. Results: The data was analyzed using One Way ANOVA and was presenting the result of the 4th day (p=0.000), 8th day (p=0.001) and 14th day (p=0.000). Post-hoc Least Significant Difference (LSD) showed that p value was less than 0.05 which means that there was a significance difference in the mean of all treatment groups. Conclusion: Toman fish (Channa micropeltes) extract possesses the capacity in decreasing the number of neutrophil on 4th, 8th and 14th day in diabetic wound healing of Wistar Rat (Rattus novergicus).
THE INFLUENCE OF TOMAN FISH (Channa micropeltes) EXTRACT ON THE NUMBER OF NEOVASCULAR IN DIABETES MELLITUS WOUND HEALING In Vivo Study on the Back of Male Wistar Rat (Rattus novergicus) Royyana, Alya; Carabelly, Amy Nindia; Aspriyanto, Didit
Dentino Vol 3, No 2 (2018)
Publisher : FKG Unlam

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Background: Patients with diabetes mellitus (DM) have delayed wound healing. This happens becauseof the depletion of neovascular number. Neovascular plays an important role in wound healing by restoringblood flow to the wound area, so the new tissue gets enough oxygen and nutrition supply to proliferate. Tomanfish (Channamicropeltes) contains albumin that can increase and decrease the number of neovascular, so it?spotentially become an alternative medicine to accelerates wound healing in DM. Purpose: The aim of thisresearch to prove the effect of toman extract dose 16 ml/Kg BW rat orally on the number of neovascular inwound of diabetic wistar rats on the 4th,8th and 14th day. Materials and methods: This research used trueexperimental method with posttest only control group design. This research used 36 wistar rats which were thendivided into 9 groups, toman fish extract dose 16 ml/Kg BW of rat group, haruan fish extract dose 13,54 ml/KgBW of rat group and BR2 feed only group. Research result: The results of One Way-Anova and Post Hoc LeastSignificant Difference tests showed there was a significant difference between group of toman extract and groupof feed only, while on group of toman extract and group of haruan extract showed there was no significantdifference on 4th,8th and 14th day. The average value of toman extract-given group on 4th, 8thand 14th day were14,75. 7,5 and 6,75 respectively. Conclusion: Toman extract has effects which are increasing the number ofneovascular on 4th day and decreasing the number of neovascular on 8th and 14th day in wound healing withDM.
THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Carabelly, Amy Nindia; Taat Putra, Suhartono; Suardita, Ketut
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 ?g/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity
Anti Inflammatory Effect Of Toman Fish (Channa micropeltes) Extract In Wound Healing Process Apriasari, Maharani Laillyza; Puspitasari, Dewi; Carabelly, Amy Nindia
Berkala Kedokteran Vol 14, No 2 (2018)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v14i2.5335

Abstract

 Abstract: One phase of wound healing is the inflammatory phase. Haruan fish extract has shown to accelerate the healing process up because it has anti-inflammatory effects. Toman fish originates from the same genus as the Haruan fish, but its anti-inflammatory effect remains unknown. It was done to analyzed anti-inflammatory effects of Toman fish extract. This study was true experimental design with posttest-only control design. Twenty-seven male mice of Balb-C strain were divided into 3 groups. Incision wound of 1 cm was made along the back of the mice. Nine mice in each group were sacrificed on 3rd days, then histopathology examination was conducted with Haematoxylin eosin. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract, and also compared to the negative control group in PMN cell examination. There were significant differences between treatment group given Toman fish extract and positive control group given Haruan fish extract compared to negative controls on the number of macrophage cells. Toman fish extract could lower the number of PMN cells and was able to increase the number of macrophages on the 3rd days. Toman fish has anti-inflammatory effects on the wound healing process. Keywords: Anti-inflammatory effects, haruan fish extract, macrophages, PMN cells, toman fish extract