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Effects of Metaldehyde and Niclosamide on The Behavior and Reproductive Capacity of Radix Quadrasi Von Moellendorf (Basommatophora: Lymnaeidae) Retnoaji, Bambang
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

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Abstract

The effects of molluscicides exposure, metaldehyde and niclosamide, on the behavior and reproductive capacity of Radix quadrasi was studied under laboratory conditions. R quadrasi were exposed to different concentration-rates of niclosamide: 0, 0.045, 0.058 and 0.075 mg/L, and of metaldehyde: 0, 100, 112 and 130 mg/L. The symptoms of poisoning of treated snails were observed closely throughout the duration of the experiment. Observations were made three times: at the beginning of treatment (0 hour), 12 hours, and 24 hours after exposure. The surviving R. quadrasi were maintained for one month to determine their reproductive capacity. Results indicated that there was immediate reaction of the snails to molluscicide exposure. Lower concentration-rates of molluscicide triggered water-leaving behavior. Such behavior occurred in all of the low and medium concentration-rates of niclosamide and metaldehyde, as well as in some snails from the control group. By contrast, higher concentration-rates caused almost instantaneous immobility. Niclosamide and metaldehyde also significantly reduced the reproductive capacity of R. quadrasi. The number of egg masses and eggs laid varied with the treatments. However, the incubation period of the treated eggs was not statistically different from the treated ones. This study showed that both niclosamide and metaldehyde significantly affected the behavior and reproductive capacity of the R. quadrasi. Between the two molluscicides used in this experiment, niclosamide was more toxic than metaldehyde.
Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) Nurhidayat, Luthfi; Arfiani, Febrina Nanda; Retnoaji, Bambang
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).
Histological Study of Phyllidia coelestis (Nudibranch) Epidermal Tissue from Pasir Putih, Situbondo Permadani, Karunia Galih; Retnoaji, Bambang
Indonesian Journal of Biology Education Vol 1, No 1 (2018): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

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Abstract

Nudibranch are a mollusk which can be found in all zones of ocean. Further, they are distributed widely from subtropical to tropical areas. The Nudibranch is characterized by lack of a shell and usually have a bright shiny color. Nudibranch has a potency to be used in the medical field because they have chemical compound inside their body. These chemical compound are the result of secondary metabolites from symbiosis with zooxanthellae in nudibranch tissues.  This research aims to study the structure of epidermal tissue of the Phyllidia coelestis collected from Pasir Putih, Situbondo, East Java Indonesia. The results showed that the epithelial structure of the Phyllidia coelestis composed of cuboidal epithelium with vacuoles spread regularly along the mantle layer below the epidermal layer. Gland cells are rarely found in the outer epithelial layer. The foot epidermal layer consisted of simple ciliated columnar epithelial cells, with goblet-shaped mucous secreting glands among the epithelium. Gill tissue structure was composed of cell layers of cuboidal ciliated epithelial cells resting on a basement membrane and connective tissues