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The influence of using platelet rich plasma for post-extraction wound healing process Oscar, Franky; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.555 KB) | DOI: 10.24198/pjd.vol22no1.14066

Abstract

Wound healing process characterized by specific processes of inflammation, proliferation, and maturation. It was coordinated by the regenerating and healing factors, including growth factor. Platelet-rich plasma was a blood component that separated from blood cell plasma, which contains a lot of growth factors for increased wound healing. The objective of this research was to observe the effect of the application of platelet-rich plasma for wound healing. The true experimental factorial design research was done to 24 rabbits (Oryctolagus cuniculus) which have been performed tooth extraction and application of platelet-rich plasma. These rabbits were terminated on 3rd, 7th, and 14th day, and the lower jaw region where the tooth had been extracted were cropped stained with hematoxylin-eosin for fibroblast and collagen histopathology examination. Based on ANOVA statistical analysis, the mean of fibroblast on the experimental group was 52.00+19.18 higher than the control group 24.83+10.46. Statistical tests showed a significant result of platelet-rich plasma application to fibroblast amount (p < 0.001). Collagen mean on the experimental group was 30.25+9.33 higher than the control group 13.75+6.96. It was significant statistically (p = 0.001). The conclusion of this research that wound healing process was influenced by the application of platelet-rich plasma.
Comparison of the effect of collagen (amino acid) and amnion (proteinase inhibitor) on the wound healing of soft tissues Adhiarto, Weko; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2000.307 KB) | DOI: 10.24198/pjd.vol22no1.14067

Abstract

The wound healing process of the soft tissue aims to accelerate the closing of the wound by recurring, restoring the function, and minimizing the scar tissue. This process has to occur in the surgery process to obtain better-wound healing. The aim of this study was to know the stimulation effect of the wound dressing to accelerate the wound healing process of the soft tissue using Collagen (amino acid) and Amnion (proteinase inhibitors). This true experimental study was done to 36 mice (Sprague Dawley) that were divided into three different groups, two groups were the treatment groups and one group was the control group. Each group was adapted in Pathology Laboratory of Faculty of Veterinary Medicine Bogor Agricultural University. All of the mice were injured on the skin by incising the right side of the back for 2 cm in length and 4 mm in depth and then cleaned with 0.9% NaCl solution. To the first group of treatment, the wound was applicated by Amnion, and the second group was applicated by Collagen. Each 4 mice of all group were a termination by ether and encryption on the wounded tissue was conducted on day 3rd, 7th, and 14th. Assessment for a number of fibroblast and collagen synthesis on the wound of back tissue was conducted with the histological examination by painted using hematoxylin-eosin. The results concluded that Collagen application was influencing the mean of fibroblast higher than Amnion, and the lowest was controlled with statistically significant. The mean of collagen used Collagen lower than Amnion, and the lowest was control with statistically significant. It could be concluded that collagen application was preeminent in increasing the productivity of the fibroblast, as well as in developing forms of the collagen synthesis compared with Amnion and control.
Temperature and holding time of instrument sterilization as an infection control of odontectomy Meliawaty, Florence; Mangundjaja, Sunardhi; Hardjawinata, Karlina
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3763.687 KB) | DOI: 10.24198/pjd.vol23no1.14059

Abstract

Odontectomy should be performed aseptically. The goal of sterilization is the complete killing of all forms of microbial life including bacterial spores on the items being processed. Biologic monitoring provides the main guarantee of sterilization. The aim of this study was to find the interrelation of the temperature and the holding time of instrument sterilization as an infection control for the successful of lower molars odontectomy. This experimental laboratory study was conducted at the Oral Maxillofacial Surgery Department in the Hasan Sadikin General Hospital Bandung and at the Microbiology Laboratory Faculty of Dentistry, Universitas Padjadjaran, Jatinangor. The Protocol was performed in three methods of sterilization: dry heat with oven and ozone, dry heat with oven and infrared (125)C for 15 minutes), both were monitored by Bacillus atrophaeus as the biologic indicators, and autoclavization (121" C for 15 minutes) with Geobaciflus stearothermophilus as the biological monitoring, with 17 times repetition. After sterilization, all of the indicators were cultured on Nutrient Agar Plate (NAPS), and the subsequent growth was assessed. The colony forming units (CFUs) were counted by Stuart Electric Bacteria Colony Counter. Adequate positive and negative controls were used in every cycle. The results showed that after autoclavization, all spores were killed. In comparison with dry heat in the oven, there were still CFUs on the NAPs, but no colonies grow after 3 repetitions by oven and infrared. Heating in oven and ozone could only reduce the spore numbers, even after repeating 5 times. The reduction of the CFUs was greater in more repetition. According to the statistical analysis, the differences were significant. This study concluded that sterilization by oven and infrared will be achieved after 3 holding times (30-35 minutes) and dry heat with oven and ozone could only act as a germicide. In autoclavization, all of Geobacillus steorothermophilus have been killed.
The influence of using platelet rich plasma for post-extraction wound healing process Oscar, Franky; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.555 KB) | DOI: 10.24198/pjd.vol22no1.14066

Abstract

Wound healing process characterized by specific processes of inflammation, proliferation, and maturation. It was coordinated by the regenerating and healing factors, including growth factor. Platelet-rich plasma was a blood component that separated from blood cell plasma, which contains a lot of growth factors for increased wound healing. The objective of this research was to observe the effect of the application of platelet-rich plasma for wound healing. The true experimental factorial design research was done to 24 rabbits (Oryctolagus cuniculus) which have been performed tooth extraction and application of platelet-rich plasma. These rabbits were terminated on 3rd, 7th, and 14th day, and the lower jaw region where the tooth had been extracted were cropped stained with hematoxylin-eosin for fibroblast and collagen histopathology examination. Based on ANOVA statistical analysis, the mean of fibroblast on the experimental group was 52.00+19.18 higher than the control group 24.83+10.46. Statistical tests showed a significant result of platelet-rich plasma application to fibroblast amount (p < 0.001). Collagen mean on the experimental group was 30.25+9.33 higher than the control group 13.75+6.96. It was significant statistically (p = 0.001). The conclusion of this research that wound healing process was influenced by the application of platelet-rich plasma.
Comparison of the effect of collagen (amino acid) and amnion (proteinase inhibitor) on the wound healing of soft tissues Adhiarto, Weko; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2000.307 KB) | DOI: 10.24198/pjd.vol22no1.14067

Abstract

The wound healing process of the soft tissue aims to accelerate the closing of the wound by recurring, restoring the function, and minimizing the scar tissue. This process has to occur in the surgery process to obtain better-wound healing. The aim of this study was to know the stimulation effect of the wound dressing to accelerate the wound healing process of the soft tissue using Collagen (amino acid) and Amnion (proteinase inhibitors). This true experimental study was done to 36 mice (Sprague Dawley) that were divided into three different groups, two groups were the treatment groups and one group was the control group. Each group was adapted in Pathology Laboratory of Faculty of Veterinary Medicine Bogor Agricultural University. All of the mice were injured on the skin by incising the right side of the back for 2 cm in length and 4 mm in depth and then cleaned with 0.9% NaCl solution. To the first group of treatment, the wound was applicated by Amnion, and the second group was applicated by Collagen. Each 4 mice of all group were a termination by ether and encryption on the wounded tissue was conducted on day 3rd, 7th, and 14th. Assessment for a number of fibroblast and collagen synthesis on the wound of back tissue was conducted with the histological examination by painted using hematoxylin-eosin. The results concluded that Collagen application was influencing the mean of fibroblast higher than Amnion, and the lowest was controlled with statistically significant. The mean of collagen used Collagen lower than Amnion, and the lowest was control with statistically significant. It could be concluded that collagen application was preeminent in increasing the productivity of the fibroblast, as well as in developing forms of the collagen synthesis compared with Amnion and control.
Temperature and holding time of instrument sterilization as an infection control of odontectomy Meliawaty, Florence; Mangundjaja, Sunardhi; Hardjawinata, Karlina
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3763.687 KB) | DOI: 10.24198/pjd.vol23no1.14059

Abstract

Odontectomy should be performed aseptically. The goal of sterilization is the complete killing of all forms of microbial life including bacterial spores on the items being processed. Biologic monitoring provides the main guarantee of sterilization. The aim of this study was to find the interrelation of the temperature and the holding time of instrument sterilization as an infection control for the successful of lower molars odontectomy. This experimental laboratory study was conducted at the Oral Maxillofacial Surgery Department in the Hasan Sadikin General Hospital Bandung and at the Microbiology Laboratory Faculty of Dentistry, Universitas Padjadjaran, Jatinangor. The Protocol was performed in three methods of sterilization: dry heat with oven and ozone, dry heat with oven and infrared (125)C for 15 minutes), both were monitored by Bacillus atrophaeus as the biologic indicators, and autoclavization (121" C for 15 minutes) with Geobaciflus stearothermophilus as the biological monitoring, with 17 times repetition. After sterilization, all of the indicators were cultured on Nutrient Agar Plate (NAPS), and the subsequent growth was assessed. The colony forming units (CFUs) were counted by Stuart Electric Bacteria Colony Counter. Adequate positive and negative controls were used in every cycle. The results showed that after autoclavization, all spores were killed. In comparison with dry heat in the oven, there were still CFUs on the NAPs, but no colonies grow after 3 repetitions by oven and infrared. Heating in oven and ozone could only reduce the spore numbers, even after repeating 5 times. The reduction of the CFUs was greater in more repetition. According to the statistical analysis, the differences were significant. This study concluded that sterilization by oven and infrared will be achieved after 3 holding times (30-35 minutes) and dry heat with oven and ozone could only act as a germicide. In autoclavization, all of Geobacillus steorothermophilus have been killed.