Articles

Found 6 Documents
Search

Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus oktiyani, neni; Sunarti, sunarti; Prasetyastuti, Prasetyastuti; Cahyono, Jujuk Anton
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.684 KB) | DOI: 10.31964/mltj.v1i1.4

Abstract

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes
ANTIHIPERGLIKEMI PATI GEMBILI (DIOSCOREA ESCULENTA) DAN EUBACTERIUM RECTALE PADA MODEL TIKUS DIABETES YANG DIINDUKSI STREPTOZOTOCIN DAN NIKOTINAMID Setyawati, Tri; Oktiyani, Neni; Kusuma, Rio Jati; Setiawan, Tony Adi; Sunarti, Sunarti
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 2, No 2 (2015)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.8 KB)

Abstract

Latar belakang. Diabetes merupakan salah satu penyakit degenaratif dengan prevalesi cukup tinggi di Indonesia. Diabetes tipe 2 memiliki prevalensi paling tinggi diantara jenis diabetes yang lain. Diabetes tipe 2 merupakan kondisi hiperglemia kronis yang umumnya disebabkan oleh resistensi insulin. Diet dengan resistant starch berpotensi untuk meningkatkan sensitivitas insulin pada penderita diabetes melalui butirat yang dihasilkan pada saat fermentasi di usus besar. Salah satu bahan pangan yang berpotensi dalam penanganan diabetes adalah gembili (Dioscorea esculenta). Eubcaterium rectale (e. rectale) merupakan bakteri butirogenik yang dapat meningkatkan produk butirat di dalam kolon.Tujuan. Tujuan penellitian ini adalah untuk mengetahui penurunan glukosa darah pada tikus Wistar yang diinduksi nikotinamide dan streptozotosin setelah pemberian diet gembili dan eubacterium rectale.Metode. Penelitian ini menggunakan desain pre dan posttest kontrol. Tikus jantan Wistar 3 bulan, dibagi menjadi 5 kelompok yaitu kelompok kontrol sehat (K1), kelompok yang diinduksi Streptozotocin (STZ) dan nikotinamide(NA) tanpa terapi (K2), kelompok yang diinduksi STZ dan NA dengan pemberian e. ractale (K3), kelompok induksi ditambah pati gembili (K4), dan kelompok induksi dengan sinbio pati gembili dan e. rectale (K5). Dengan lama intervensi 4 minggu.Hasil. Terjadi penurunan kadar glukosa darah setelah intervensi 4 minggu yaitu antara kelompok K2 dan K3, K4 dan K5 dengan nilai signifikansi p < 0,05.Kata kunci: diabetes tipe 2, Dioscorea esculenta, resistant starch, butirat, resistensi insulin, sensitivitas insulin.
Effects of Dioscorea esculenta and Eubacterium rectale on insulin receptor substrate 1 (Irs1) Expression in skeletal muscle and homeostatic model assessment-insulin resistance (HOMA-IR) in diabetic rats Sunarti, .; Setyawati, Tri; Oktiyani, Neni; Kusuma, Rio Jati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 3 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.589 KB)

Abstract

Low expression of insulin receptor substrate 1 (Irs1) is associated with insulin resistance and type 2 diabetes mellitus (type 2 DM). This study was performed to evaluate the effects of Dioscorea esculenta and Eubacterium rectale on the Irs1 expression in the skeletal muscle and the homeostatic model assessment-insulin resistance (HOMA-IR) of diabetic rats. Twenty-five male Wistar rats were divided into five groups i.e. non diabetic rats Group 1; diabetic rats as Group 2; diabetic rats + D. esculenta as Group 3; diabetic rats + E.rectale as Group 4 and diabetic rats + both E. rectale and D. esculenta as Group 5. Rats were made diabetic with induction of intraperitoneally injection of nicotinamide and streptozotocin. After four weeks of the interventions, the blood and skeletal muscles were taken. The Irs1 expression was analyzed with immunohistochemical staining, plasma glucose levels was analyzed using a spectrophotometer, and insulin was analyzed using ELISA methods. All intervention groups reduced plasma glucose levels and HOMA-IRs (p<0.001) and increased Irs1 expression. The greatest reduction of  plasma glucose levels and increase of Irs1 expression in the skeletal muscle were found in Group 4, however, the lowest of HOMA-IR was seen in Group 5. These results suggested that D.esculenta, E.rectale, and the combination reduced plasma glucose levels and HOMA-IR by increasing Irs1 expression in skeletal muscle.
Akurasi Hitung Jumlah Eritrosit Metode Manual dan Metode Otomatis Oktiyani, Neni; fahriyan, fahriyan; Muhlisin, Akhmad
Medical Laboratory Technology Journal Vol 3, No 2 (2017): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.051 KB) | DOI: 10.31964/mltj.v3i2.166

Abstract

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.
Lactic Acid Levels Yogurt Red Beans with Addition of Honey Trigona sp Widyati, Ruri; Lutpiatina, Leka; Oktiyani, Neni; Haitami, Haitami
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (754.872 KB)

Abstract

Red beans (Phaseolus vulgaris L.) are grains that are rich in nutrients and useful as a substitute for vegetable milk which can be processed into yogurt using Streptococcus thermophillus and Lactobacillus bulgaricus. Carbohydrates in red beans consist of a group of oligosaccharides that have little ability as an energy source for bacteria to produce lactic acid. Therefore, the making of red bean yogurt needs another source of sugar by adding Trigona sp. Honey. The purpose of this study was to determine the effect of adding Trigona sp honey by 0%, 1.9%, 3.8%, 5.7%, 7.6%, 9.5% to the total level of lactic acid in red bean yogurt incubated for 24 hours at 37oC. This type of research is an experiment with the Postest Only With Control Group Design research design using the alkalimetry titration method. The sampling technique used was purposive sampling technique, namely Trigona sp honey bee taken at Trigona sp bee farm in Tambangan, Tanah Laut. Indonesia. The results showed that there was an effect of adding Trigona sp honey to total lactic acid levels in red bean yogurt with the addition of Trigona sp honey 1.9% - 9.5% fulfilling the requirements of SNI 01.2981-2009. The highest average value of total lactic acid in red bean yogurt with the addition of Trigona sp honey 9.5% which is equal to 0.6672%. It is recommended for future researchers to use other types of carbohydrate sources and additives in the form of proteins. For the community, it is recommended to use alternative sources of other sugar and optimize the addition of Trigona sp. Honey.
Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Nurlailah, Nurlailah; Alma, Nurhayati Aslami; Oktiyani, Neni
Medical Laboratory Technology Journal Vol 3, No 1 (2017): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.806 KB) | DOI: 10.31964/mltj.v3i1.148

Abstract

Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.