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Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi Dwiyanti, Ratih Dewi; Nurlailah, Nurlailah; Widiningsih, Indah Kurnia
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi. Keywords: Water decoction of leaves Binahong, Salmonella typhi, antibacterial. Abstrak: Penyakit tifus atau dikenal dengan demam tifoid atau demam enterik adalah salah satu penyakit demam akut yang disebabkan oleh bakteri Salmonella typhi. Pengobatan demam tifoid biasanya menggunakan antibiotik, penggunaan antibiotik dapat menimbulkan efek samping. Masyarakat saat ini banyak menggunakan pengobatan dengan bahan alami, salah satunya adalah Binahong (Anredera cordifolia) yang mengandung senyawa Alkaloid, Polifenol, Flavonoid, Saponin, dan Antrakuinon yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efektifitas air rebusan daun Binahong terhadap pertumbuhan Salmonella typhi secara in vitro. Jenis penelitian ini adalah true eksperiment dengan rancangan penelitian Posttest Only Control Group Design dan metode yang digunakan adalah difusi (sumuran) dengan 5 perlakuan. Konsentrasi air rebusan daun Binahong yang dgunakan adalah 20%, 40%, 60%, 80% dan 100%. Hasil penelitian didapatkan zona hambat air rebusan daun Binahong terhadap Salmonella typhi pada konsentrasi 20%, 40%, 60%, 80% adalah 0 mm, sedangkan pada konsentrasi 100% didapatkan zona hambat sebesar 11 mm. Kesimpulan penelitian ini adalah air rebusan daun Binahong pada konsentrasi 100% memiliki kemampuan menghambat pertumbuhan Salmonella typhi, namun hasil ini belum efektif karena masih dalam kategori resisten. Disarankan bagi peneliti selanjutnya untuk meningkatkan konsentrasi air rebusan daun binahong atau menggunakan ektrak alkohol daun binahong untuk menghambat pertumbuhan Salmonella typhi. Kata kunci : Air rebusan daun Binahong, Salmonella typhi, antibakteri.
Staphylococcus aureus pada keyboard di unit pelayanan penunjang rumah sakit Putri, Deby Arnisya; Roebiakto, Erpan; Dwiyanti, Ratih Dewi
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

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Abstract

Computer keyboard can be a cause of health problems due to poor cleanliness. Keyboard that is not cleaned contains more bacteria. Staphylococcus aureus is one of the bacteria that causes nosocomial infection that can be found on the keyboard. The purpose of this research is to know the description of Staphylococcus aureus bacteria in Hospital Supporting Unit. This type of research is descriptive survey with 16 samples with total sampling. Based on research conducted on the keyboard in the Medical Support Unit Hospital found Staphylococcus aureus as much as 68.75% It is recommended to do keyboard cleanliness and cleanliness of computer users before and after using the computer.
Daya Hambat NaCl terhadap Pertumbuhan Staphylococcus aureus Amalia, Amalia; Dwiyanti, Ratih Dewi; Haitami, Haitami
Medical Laboratory Technology Journal Vol 2, No 2 (2016): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Staphylococcus aureus is a pathogenic bacterial that causes infectious disease and human poisoning through enterotoxin produced by the bacteria. Salt or sodium chloride is used by human for food preservation process because it can inhibit the growth of bacteria, especially Staphylococcus aureus. The purposes of the study were to determine the effect of NaCl in media on the growth of Staphylococcus aureus and determine the effective concentration of NaCl. The type of research used in this study was true experiment posttest only control group design. Samples were prepared in 5 NaCl concentrations, they were 10%, 15%, 20%, 25% dan 30%. The study conclusion was there were effects of NaCl addition to the media on the growth of Staphylococcus aureus with a significance value of 0.000 (
Lime Soap as Bacteria Reducer Dwiyanti, Ratih Dewi; Lutpiatina, Leka
Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).
Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp Dwiyanti, Ratih Dewi; Dediq, Rion; Thuraidah, Anny
Medical Laboratory Technology Journal Vol 3, No 1 (2017): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

edes sp is known as a mosquito that transmits various diseases. In addition to spreading dengue fever and chikungunya, these mosquitoes also spread the virus zika is quite dangerous. One of the effort to controlling these diseases is to eradicate the vector by using materials that can be taken from plants one of them is bay leaf (Syzygium polyanthum). The purpose of this study is to determine the kills power of Syzygium polyanthum water extract against Aedes sp larvae. This research uses true experiment with Posttest Only With Control Group Design design. Data were obtained from the number of larval deaths in the Syzygium polyanthum extract in each control group and treatment group for 24 hours. The results showed that the percentage of bayleaf water extract which has the largest number of larval deaths was at 32% concentration with 55% death percentage. It was concluded that each concentration of aqueous bay leaf water extract given had a killing power against Aedes sp larvae with the lowest percentage of 2.5% and the highest 55%. Based on the result of Kruskal-Wallis test showed that water extract of the bay leaf was able to kill Aedes sp larvae at each treatment with the value of sig 0,001. Suggestion for the next researcher to test the power of killing the water extract of Syzygium polyanthum to Aedes sp larvae by increasing the concentration of salted bay water extract.
DAYA HAMBAT EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus aureus Lutpiatina, Leka; Amaliah, Nur Rizqi; Dwiyanti, Ratih Dewi
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

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Abstract

Daun kenikir (Cosmos caudatus Kunth.) sudah lama dimanfaatkan masyarakat untuk dikonsumsi maupun pengobatan tradisional. Daun kenikir memiliki kandungan fenol, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal dan konsentrasi bunuh minimal ekstrak daun kenikir terhadap bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat true eksperiment dengan rancangan postest only control group design. Variabel bebas dalam penelitian ini adalah konsentrasi ekstrak daun kenikir (Cosmos caudatus Kunth.).  Variabel terikat dalam penelitian ini adalah bakteri Staphylococcus aureus. Hasil penelitian Konsentrasi Hambat Minimal (KHM) menunjukkan kekeruhan pada konsentrasi 160 mg/ml, 320 mg/ml dan adanya kejernihan pada konsentrasi 340 mg/ml, 380 mg/ml dan 400 mg/ml. Hasil penelitian Konsentrasi Bunuh Minimal (KBM) diperoleh jumlah koloni pada konsentrasi 160 mg/ml sebanyak 60 CFU/ml, 320 mg/ml sebanyak 12 CFU/ml, 340 mg/ml sebanyak 3 CFU/ml, 380 mg/ml sebanyak 0 CFU/ml dan 400 mg/ml sebanyak 0 CFU/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa KHM ekstrak daun kenikir adalah 340 mg/ml dan KBM ekstrak daun kenikir adalah 380 mg/ml. Disarankan dilakukan penelitian lebih lanjut mengenai pengaruh ekstrak daun kenikir terhadap bakteri Staphylococcus aureus adalah dengan menggunakan pelarut lain.
Fungi That Produce Toxins in Salted Fish Susanti, Yuli; Lutpiatina, Leka; Dwiyanti, Ratih Dewi
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

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Abstract

Salted fish are fish that are processed through a process of salting and drying. The contamination of fungi in salted fish can be caused by prolonged storage. Storage of salted fish that is too long can cause the growth of various fungi. One of the fungi that often grows in salted fish is the fungus of Aspergillus sp. Some species of the Aspergillus sp fungi can produce aflatoxin, one of which is Aspergillus flavus. This study aims to determine the contamination of toxin-producing fungi in salted fish in the traditional Banjarbaru market in Indonesia. The type of research used is descriptive survey. Samples were taken by purposive sampling taken from 5 salted fish sellers each taken 3 different types of salted fish so that the number of samples was 15. The results were obtained from 15 samples examined, 6 positive samples contaminated with Aspergillus flavus fungi, 8 positive samples contaminated with Aspergillus fungi niger, 5 positive samples contaminated with Monilia sitophila fungi, 6 positive samples contaminated with Rhizopus sp fungi, 6 positive samples contaminated with Penicillium sp fungi, and 1 positive sample contaminated with Mucor sp fungi. Based on the results of the study, samples of salted fish contaminated with Aspergillus sp fungi were 73% (11 samples) and no samples were contaminated with Fusarium sp.
Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Dwiyanti, Ratih Dewi; nailah, hana; Muhlisin, Ahmad; Lutpiatina, Leka
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

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Abstract

       Jeruk nipis (Citrus aurantifolia) dapat digunakan untuk obat batuk, peluruh dahak, influenza, dan obat jerawat. Jeruk nipis mengandung senyawa kimia yang bermanfaat salah satunya minyak atsiri dan flavonoid yang berfungsi sebagai antibakteri dan berperan sangat penting dalam menghambat pertumbuhan bakteri. Tujuan penelitian ini untuk mengetahui efektivitas air perasan jeruk nipis (Citrus aurantifolia) pada konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% dan 100%  terhadap pertumbuhan Eschericia coli secara in vitro. Penelitian ini bersifat  eksperimen dengan rancangan post test only control group design. Sampel penelitian adalah air perasan jeruk nipis. Pengujian daya antibakteri menggunakan metode difusi sumuran. Parameter daya antibakteri ditentukan dengan mengukur zona hambat  (mm) yang terbentuk di sekitar pertumbuhan bakteri uji pada media Muller Hinton Agar. Hasil Penelitian menunjukkan zona hambat air perasan jeruk nipis terhadap pertumbuhan Eschericia coli pada konsentrasi 40%, 50%, 60%, 70%, 80%, 90% dan 100% masing- masing berdiameter 7,25mm, 13,25mm, 14,25mm, 16mm, 17mm, 18,25mm, dan 20,75mm. Berdasarkan uji regresi linear didapat nilai signifikan 0,000 < α 0,05 sehingga dapat disimpulkan bahwa ada pengaruh air perasan jeruk nipis terhadap pertumbuhan Eschericia coli secara in vitro.  Disarankan pada penelitian lebih lanjut untuk menguji daya hambat air perasan jeruk nipis dengan menggunakan metode lain atau terhadap bakteri spesies lain.
Angka Kuman Es Batu Produksi Rumah Tangga Yulianti, Annisa Nur; Dwiyanti, Ratih Dewi; Norsiah, Wahdah; Lutpiatina, Leka
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

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Abstract

Air adalah kebutuhan dsar manusia, baik untuk keperluan sehari-hari seperti mandi, cuci, masak, dan minum. Air tidak hanya untuk air minun saja, tetapi dapat dibuat es batu yang memenuhi syarat bakteriologis. Penelitian bertujuan untuk mengetahui hasil TPC (Total Plate Count) pada es batu produksi rumah tangga di Kelurahan Sungai Besar. Penelitian ini bersifat survei deskriptif, sampel diperiksa dengan menggunakan metode TPC (Total Plate Count)  dengan pengenceran 100 sampai 10-4. Sampel pemeriksaan berupa es batu yang diambil dari rumah produksi di Kelurahan Sungai Besar yang berjumlah 6. Hasil penelitian dari 24 sampel es batu produksi rumah tangga di Kelurahan Sungai Besar adalah 2 sampel yang memenuhi syarat dan 22 sampel tidak memenuhi syarat. Kesimpulan dari penelitian ini adalah 80% sampel tidak memenuhi syarat Standar Nasional Indonesia (SNI) 01-3553-2006. Saran penelitian lebih lanjut dengan melakukan identifikasi terhadap jenis bakteri yang terdapat dalam es batu tersebut.
Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru Dwiyanti, Ratih Dewi; Lutpiatina, Leka
Medical Laboratory Technology Journal Vol 2, No 1 (2016): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.