Laily Ilman Widuri, Laily Ilman
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A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP Widuri, Laily Ilman; Dewanti, Parawita; Sugiharto, Bambang
BIOVALENTIA: Biological Research Journal Vol 2, No 1 (2016)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

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Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.
Potret Budidaya Padi Lebak oleh Petani Lokal di Kecamatan Pemulutan, Ogan Ilir, Sumatera Selatan Lindiana, Lindiana; Lakitan, Benyamin; Herlinda, Siti; Kartika, Kartika; Widuri, Laily Ilman; Siaga, Erna; Meihana, Meihana
Jurnal Lahan Suboptimal Vol 5, No 2 (2016): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Lindiana, et al. Rice Cultivation Images by Local Farmers in Pemulutan District, Ogan Ilir, South Sumatra. JLSO 5(2):153-158. Plans and efforts in increasing rice productivity at riparian wetlands can be commenced by introducing relevant technologies; however, the selected technologies should be based on real needs or problems faced by local farmers. As a pre-requisite, if the needs and problems are not comprehensively understood, government intervention to introduce new technology may not be effective since the technology may not be relevant or affordable to local farmers. Objectives of this research are to analyze characteristics of the wetlands, farmer’s adoption capacity, and technological preferences. The research was conducted in five villages at Pemulutan District. Qualitative Grounded Theory and Quantitative Survey were employed through dialogues with 100 local farmers.  Results of the research indicated that any effort to increase riparian wetland productivity should consider the unpredictability of flood occurrences and prolonged drought. These unfavorable conditions have limited local farmers to only grow rice once per year. Local farmers were almost solely depending on rice and cultivation of other crops were rarely observed, except limited vegetables grown on elevated border of paddy fields.