The Comparison Of Additional Bio-Activator EM-4 (Effective Microorganism) And Mol (Microorganisme Local) Pineapple (Ananas Comosus L.Merr) Skin To The Composting Time. Composting is the reform process (decomposition) and stabilization of organic materials by microorganism in controlled environment (controlled) and its final outcome of humus or compost. It can be speed up by addition other ingredients called the activator. Activator is material consisting of enzymes and microorganisms (bacterial culture) that can accelerate the composting process. This study aimed to determine the effect of additional bioactivator EM-4 (Effective Microorganism) and MOL (Microorganism Local) pineapple skin (Ananas comosus l.merr) skin to the composting time. They was administered on each treatment and observed for 24 day. The result of the compost with the addition bio-activator EM-4 took for 16 days and compost with the addition MOL pineapple skin took for 14 days. Based on the statistical analysis by One Way Analysis of Variance method with α = 0.05 was significant effect between the addition of bio-activator EM-4 and MOL pineapple skin to the compositing time with average composting time for 16 and 14 days. Further study can be conducted by potential materials as basic raw materials for MOL such as fruits (banana, sugar cane, jackfruit), animal and human urine, and food scraps.

Abstract: Knowledge, Attitude with Merchants Participation In The Management of Market Waste. In Banjarbaru city waste volume transported by TPS officers per day about 90 tons / day to TPA. One of the source of waste is the market which is a big problem because most of the market waste is wet garbage. So these waste piles become flies nest, rats, insects.Waste management is also influenced by the participation of merchants that were still lacking awareness to play an active role in the implementation. The purpose of this study is to determine the relations of knowledge, attitude with the participation of merchants in waste management in the Bauntung market Banjarbaru. The type of this research is analytical survey research with Cross Sectional approach. The population in this study is all merchants in the Bauntung market Banjarbaru. The sample is 85 respondents, obtained by proportional sampling method. The research variables consist of independent variables, namely knowledge and attitude while the dependent variable is the participation of merchants in waste management. Data analysis used is univariat and bivariate (using Chi Square with α = 0,05). The result of the research stated that there is no correlation between knowledge level and participation (p-value 0,747> 0,05). There is a correlation between attitudes with participation (p-value 0.001

Abstract : Decreased Levels BOD, COD and TSS Waste Of Tahu Using Effective Microorganism-4 (EM4) In Aerobic. Wastewater was generated from industry tahu still contains levels of polluters such as BOD, COD and TSS are still high that can pollute waterways, Therefore it must be lowered levels before discharge into waterways. One way to do is wastewater treatment with EM4. This type of research was a quasi experiments aimed to determine the reduced levels of BOD, COD and TSS waste of tahu with wastewater treatment using the activator Effective Microorganism-4 (EM4) in aerobic with variation concentrations of 0%, 3%, 5%, 7% and retention time of 72 hours, 144 hours, 216 hours. The results showed the highest decrease levels wastewater concentration treatment of 7% EM4 with retention time of 216 hours with a BOD value 399,9 mg/L (88,8%), COD 1355,2 mg/L (85,3%) and TSS 287 mg/L (72,7%). Data analysis used two-way anova in the treatment group EM4 concentration and retention time obtained p value = 0.000 and α = 0.05 the results showed that there were significant differences due to variations in the concentration of EM4, due to the retention time and due to the effects of interactions EM4 concentration with retention time. The treatment results still exceeded the quality standard so before doing aerobic treatment, the first can do anaerobic treatment, so that wastewater treatment is getting better effluent. Keywords: Waste of tahu; levels of BOD; COD;TSS; EM4; retention time.

Abstract: LC 50Test of Tofu Waste Water to Tilafia (Orechromis niloticus) aged 2 months in Banjarbaru. Tofu industrial wastewater with a low pH, 0 mg/l DO and a temperature of 32°C when discharged without treatment into receiving water bodies will lead to the death of aquatic biota so acute toxicity tests need to be done. This study aims to determine the LC50 of the waste out in tilapia (Oreochromis niloticus). This study used an experimental method, posttest design with a control group. Samples were taken randomly in waste outlet in Banjarbaru, then toxicity tests performed with various of toxicant concentrations and exposure time at tilapia of 2 months age.  Based on the initial results of toxicity tests, the lowest concentration that causes 100% dead of tilapia is 20%, so that the concentration of industrial wastewater know that will be used in the acute toxicity tests are: 4, 8, 12, 16 and 20%. Parameters wich were controlled are pH, temperature and DO. Probit  analysis using SPPS was used to determined LC50. The results showed that LC50 for exposure time: 24, 48 and 72 hours are 3.80 up to 11.35%, 3.67 s.d 14.30% and 3.38 up to 12.10% particularly. Correlation analysis showed the characteristics of pH and DO in the tofu industrial wastewater affects the LC50. Decrease of DO and pH, LC50 would decrease. The study is expected as input to information and pollution control and waste out policy decisions in order to make local regulations maintained environmental sustainability. For further research can complement parameters Ammonia levels and turbidity in the water that affect the survival of fish, in addition to the pH, DO and temperature of the water.Keywords   : LC50 test; tofu waste water; Tilafia (Orechromis niloticus) of 2 months aged.

Abstrak: Nilai resistivitas dengan variasi jarak di tempat pemrosesan akhir sampah Gunung Kupang Banjarbaru. Tempat Pemrosesan Akhir Sampah (TPA) Gunung Kupang Banjarbaru terletak di Kecamatan Cempaka.  Pembuangan  sampah di TPA ini rata rata  1000 m3 atau 65 - 85 ton per hari.  Pada daerah ini diduga terdapat rembesan air lindi.  Tujuan penelitian ini diperolehnya gambaran pola distribusi akumulasi rembesan air lindi di sekitar TPA Gunung Kupang berdasarkan nilai resistivity (tahanan jenis listrik). Metode penelitian secara  eksperimen    yaitu dengan menetapkan variasi jarak dari lahan Tempat Pemrosesan Akhir Sampah kemudian dilakukan   pengukuran nilai resistivity lapisan tanah dan batuan. Perlakuan yang dilakukan adalah variasi jarak dari TPA mulai dari 0, 10 meter dan 40 meter sedangkan variabel yang diteliti adalah nilai resisistivitas dari lokasi yang berhubungan TPA. Hasil penelitian menunnjukkan bahwa rerata nilai Resistivitas pada lintasan A (0 m dari TPA ) 13.075Ωm , pada lintasan B (10 m dari TPA ) 30.995 Ωm  dan pada Lintasan C (40m dari TPA ) 91.3 TPA 91.3 Ωm . Ada perbedaan bermakna rata-rata nilai Resisistivitas (p= 0.056). Rata-rata nilai resistivitas lintasan A(0 m dari TPA) dengan Lintasan B (10 m dari TPA). tidak ada perbedaan bermakna (p= 0.74) dan ada rembesan lindi pada jarak 10 m dari TPA. Rata-rata nilai resistivitas lintasan A(0 m dari TPA) dengan Lintasan C (40 m dari TPA) ada perbedaan bermakna (p= 0.027). Rembesan cairan lindi  terbanyak memanjang dari jarak 0 sampai 100 meter pada lintasan A memanjang pada jarak 60 – 72.5 m pada lintasan B dan pada lintasan C memanjang pada jarak 80 – 140 m, dengan nilai resistivitas antara 2 – 8 Ωm. Disarankan penggunaan metode penyelidikan geolistrik konfigurasi Dipole dipole  Res2Dinv D bisa dipakai untuk evaluasi  tentang kemungkinan cemaran lindi dari Tempat pemrosesan Akhir sampah. Kata Kunci : Resistivitas, Jarak dan Tempat Pemrosesan Akhir Sampah

Abstract: Effect Of Addition Bio-Activator EM-4 (Effective microorganism) And Mol (Microorganism Local) Casserole Rice On Timing Of Compost. Composting is the decomposition of organic matter into simple materials is done naturally with the help of microorganisms , but the composting process that occurs naturally long and slow so indispensable ingredient bioactive able to accelerate the composting process. The help of a solution of EM - 4 (Effective microorganism) and MOL (Local Microorganisms) rice casserole that can be accelerated < 2 months . This study aims to determine differences in the addition of EM - 4 bio-activator (Effective Microrganism) with MOL (Micro Organism Local) Rice Casserole to the time of the compost. The method used in this study is experimental which has been experimenting with the observed variables such as time of compost from each - each treatment well control , EM - 4 as well as the addition of a solution MOL stale rice. Then after the data collected will be followed by a test using a One Way Analisys of Variance , the provisions of P value < alpha (0.05). The results showed that the value of p < alpha (0.05) . So there is the effect of the time difference with the help of compost activator EM-4/MOL rice. Then the three treatments of the fastest time of the end of the composting process is treatment with the addition of a solution of the stale rice MOL (15 days). Based on the results of the study can be recommended to accelerate the composting time can be used activator in the form of EM - 4 or with the help of a solution-based MOL stale rice. Keywords: Compost; EM – 4; MOL Rice Basi; Time Occurrence Compost.

Production of Biogas from Cow Manure and Chicken Manure with Addition of Papain. Farms not only contribute positively to meet the demands of protein and increase incomes but also have a negative impact on the environment due to their wastes, especially manure. Biogas produced from the anaerobic decomposition of organic waste is a solution of manure utilization to be a fuel which is cheap and environmentally friendly. To accelerate the production of biogas it is needed a proteoloitik enzyme from papaya, which is called papain. This study aims to calculate the production of biogas from cow manure and chicken manure with addition of papain. The experiments were conducted in the digester made from plastic drums and observed for 30 days. The results showed that the papain influenced of production acceleration of biogas from cow manure. Comparison of biogas production in a week from cow manure: cow manure + 2 kgs of papaya pieces: cow manure + 4 kgs of papaya pieces are 202.7 liters: 263 liters: 332.3 liters. Cow manure did not need other ingredients except water with ratio 1: 1½ . Chicken manure had not been able to produce biogas because their CN ratio was not eligible, although the chaff had been added to increase the carbon content. Biogas from cow manure with addition of papain can be applied on a wider scale, while biogas from chicken manure requires other ingredients that biodegrade easier to add C content in the mixture. Keywords : biogas; cow manure; chicken manure; papain

Rational empirical antimicrobial therapy is an important component of sepsis patient management. This study aimed to assess the rationality of empirical antimicrobial therapy in patients diagnosed with sepsis admitted in intermediate care ward of internal medicine department (RPI) of Dr. Soetomo General Hospital from January 2016 to July 2017. Medical records of 91 patients diagnosed with sepsis were collected and studied retrospectively in period from July 2017 to November 2017. 91 (85.05%) medical records from 107 sepsis patients were evaluated. Cultures and antimicrobial sensitivity tests were carried out in 21 (23.07%) patients. 14 patients yielded positive culture results, 9 of which were MDRO positive with ESBL as resistant marker. Empirical antibiotic therapies for these patients were reviewed according to Gyssens method.73 (80.2%) of 91 patients were deemed receiving appropriate empirical antibiotic therapies. Ceftriaxone IV injection as monotherapy or combination therapy were the most common empirical antibiotic therapies (82 in 91 patients, 90.1%), despite local microbiologic flora and antibiogram show most pathogens were resistant to ceftriaxone. Mortality rate in this study was high, 92.3% (84 patients died) despite rational empirical antibiotic therapies were high. This study concluded that empirical antibiotic therapies in sepsis patients according to guidelines adopted in Soetomo General Hospital, albeit deemed rational, was no longer appropriate according to local antibiogram issued by microbiological department of Soetomo General Hospital. Keywords: Empirical Antibiotics Therapy, Gyssens criteria, Intermediate Care Ward, Sepsis, Septic Shock

Carbapenemase-producing Escherichia coli (E. coli) has caused trouble in therapeutic antibiotic selection. Carbapenemase screening procedure in laboratories is usually based on inacurate semi-automatic system. Confirmation and classification of carbapenemases according to Ambler can be done with combination of phenotypic methods, i.e., Modified Hodge Test (MHT), Sodium Mercaptoacetic Acid (SMA), and 3-Aminophenylboronic Acid (PBA). This study aimed to compare profiles of carbapenemase-producing E. coli which were confirmed and classified phenotypically with the genotypic profiles. E. coli isolates from urine specimens which were potential as carbapenemase-producers according to semi-automatic system BD Phoenix were phenotypically tested with MHT, SMA, and PBA. Isolates were grouped as carbapenemase-producers and non carbapenemase-producers. Phenotypic carbapenemase-producer isolates were classified based on Ambler criteria. All isolates were then tested with Polymerase Chain Reaction (PCR) for the presence of OXA-48, IMP1, IMP2, GES, VIM, NDM, KPC genes. Out of 30 isolates, 6 isolates (20.0%) were MHT positive, and 25 isolates (83.3%) were SMA positive, which indicated that most isolates produced were carbapenemase Ambler B. PCR confirmed 12 isolates (40.0%) had VIM gene which were classified as carbapenemase Ambler B. Phenotypic confirmatory test had 100% sensitivity and 22.2% specificity. Classification with phenotypic confirmatory test had 91.7% match with PCR. Phenotypic confirmatory test detected more carbapenemase than PCR. This low specificity may be caused by inappropriate use of diagnostic gold standard. PCR should not be used for routine carbapenemase confirmation because of vast diversity of carbapenemases. Phenotypic confirmatory test can classify carbapenemase according to Ambler classification.

Village of Jambu  Hilir  is one endemic Dengue Hemorrhagic Fever (DHF) in Hulu Sungai Selatan Distric . Data from Puskesmas Jambu Hilir  In 2011, there were 15 cases of DHF and in 2012 there were 34 cases of dengue fever, in 2013 there were 37 cases of one person died and in 2014 to 8 cases of DHF, and in 2015 increased to 58 cases of DHF, and 1 people died, while Figures Free Flick (ABJ) 67.9% lower (<95%). This study aims to determine the relationship between knowledge about mosquito nest eradication (PSN DBD) with the presence of larvae of Aedes aegypti (Ae. Aegypti) households in the village of Jambu Hilir.  Cross sectional study was conducted in 64 households, selected by simple random sampling of 236 households. Respondents are heads of households. The independent variable was public knowledge about the PSN while the dependent variable was the presence of mosquito larvae Ae. aegypti. Data were collected by interviews face advance individually housed in homes using questionnaires and observation. Data analysis was performed using univariate, bivariate and analyzed using Chi – square Test.  Results showed knowledge about PSN good category 31.3%, 14.1% lacking  and sufficient 54.7%. The house is positive there Aedes sp larvae  37.5% =  ABJ is 62.5%. Statistically significant relationship PSN level of public knowledge about the existence of Aedes sp larvae in the home (p = 0:00). It is suggested that efforts to change behaviors that support PSN DBD is still needed. Puskesmas need to increase health promotion on the dangers of dengue and its prevention, coordination across relevant sectors and the performance of larva monitoring (jumantik) to improve the PSN behavior of households, resulting in increased morbidity,  ABJ and a drop in dengue. Keywords:Knowledge PSN, free numbers larva rate, Aedes aegypti, DHF