Fitriyanti Jumaetri Sami, Fitriyanti Jumaetri
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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (2,2 diphenyl-1-picrylhydrazyl) dan METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat) Sami, Fitriyanti Jumaetri; Rahimah, Sitti
Jurnal Fitofarmaka Indonesia Vol 2, No 2 (2015): JURNAL FITOFARMAKA INDONESIA
Publisher : FAKULTAS FARMASI UNIVERSITAS MUSLIM INDONESIA

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Abstract

Telah dilakukan penelitian uji aktivitas antioksidan ekstrak metanol bunga brokoli (Brassica oleracea L. var. Italica) dengan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan metode ABTS (2,2-Azinobis (3-etilbenzotiazolin)-6-asam sulfonat). Bunga brokoli diekstraksi secara maserasi dengan menggunakan pelarut metanol.  Ekstrak bunga brokoli (Brassica oleracea L. var. Italica) dibuat dalam berbagai konsentrasi dan diuji aktivitas antioksidannya. Pada metode DPPH diperoleh nilai IC50 sebesar 123,698 ppm dan IC50 vitamin C murni sebesar 3 ppm sedangkan pada metode ABTS diperoleh nilai IC50 sebesar 32,1292 ppm dan nilai IC50 vitamin C murni sebesar 6 ppm.
UJI AKTIVITAS TABIR SURYA PADA BEBERAPA SPESIES DARI FAMILY ZINGIBERACEAE DENGAN METODE SPEKTROFOTOMETRI Sami, Fitriyanti Jumaetri; Nur, Syamsu; Martani, Megawati M.
Jurnal Ilmiah As-Syifaa Vol 7, No 2 (2015): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Abstract

Testing activity sun screen of several species from family Zingiberaceae with a spectrophotometric method have been. Study purpose is to obtain data on the sunscreen activity of ginger extract Z. officinale var. Officinale, Z. officinale var. Rubrum and Zingiber zerumbet SM and their combination with the parameters of % transmission of erythema, % transmission of pigmentation and SPF. The method used an experimental laboratory. Evaluation of the activity of ethanol extract of Z. officinale var. Officinale, Z. officinale var. Rubrum and Zingiber zerumbet SM made by maceration method using 70% ethanol. The test is based on the ability of the absorb the rays ultraviolet. Research carried out by the respective concentrations of ethanol extract of Z. officinale var. Officinale, Z. officinale var. Rubrum and Zingiber zerumbet SM of 200 ppm , 400 ppm , 600 ppm, 800 ppm and 1000 ppm. Based on percent transmission of erythema and percent transmission of pigmentation at a concentration of 1000 ppm, respectively ethanol extract Z. officinale var. Officinale, Z. officinale var . Rubrum and Zingiber zerumbet SM could be used as a sunscreen but still weak to be used as sunscreen ingredients. The result showed that combined Z. officinale var. Officinale, Z. officinale var. Rubrum and Zingiber zerumbet SM at a concentration of 500 ppm obtained percentage value of 1% transmission of erythema and 3,9% of pigmentatied transmission and categories as sunblock with SPF value is 50 protection.
PEMANFAATAN JAMUR ENDOFIT DARI DAUN MURBEI (Morus alba L.) SEBAGAI ANTIBIOTIK Kursia, Sukriani; Ali, Alimuddin; Sami, Fitriyanti Jumaetri; Adhawiyah, Rabiatul
Jurnal Ilmiah As-Syifaa Vol 9, No 1 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Endophytic fungi are fungi colonize internally plant tissue, without giving detrimental effect to the host plant. They act as symbiont, mediated plant resistance against biotic stress i.e. pests and diasease and abiotic stress such as drought and extreme of temperature. Endophytic fungi are one of the most creative groups of secondary metabolite producers that play important biological roles for human life. Fungi are among the most important groups of eukaryotic organisms that are well known for producing many novel metabolites which are directly used as drugs or function as lead structure for modification.Mulberry (Morus alba L.) is one of the genus species known to have secondary metabolite compound that can be used as an antioxidant, antibacterial, antiviral, antidiabetic, antihypertensive, hypolipidemic, fever, flu, cough, antiemetic and gastrointestinal disorders. This research aimed to determine antibacterial activity of Mulberry Fungi from the leaves against the bacteria Escherichia coli and Staphylococcus aureus. Isolation of endophytic fungal carried by the direct isolation techniques. Isolates which produced as much as 3 isolates the MUR1, MUR2 and MUR3. Further isolates fermented using PDY medium and extracted using ethyl acetate. The ethyl acetate extract qualitative test the activity against bacteria Escherechia coli and Staphylococcus aureus. The result of the antibacteria activity, ethyl acetat extract from fermentation medium PDY of Mulbery (Morus alba L.) inhibited growth of Escherechia coli and Staphylococcus aureus at > 12 mm Keywords: Endophytic fungi, Mulberry (Morus alba L.), Antibiotic.
UJI AKTIVITAS ANTIOKSIDAN DAUN KERSEN (Muntingia calabura L.) DENGAN METODE DPPH (1,1-difenil-2-pikrilhidrazil) DAN FRAP (Ferric Reducing Antioxidan Power) Sami, Fitriyanti Jumaetri; Nur, Syamsu; Ramli, Naimah; Sutrisno, Budi
Jurnal Ilmiah As-Syifaa Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

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Abstract

Has conducted research of antioxidant activity from kersen leaves (Muntingia calabura Linn) using DPPH (1,1-diphenyl-1-picrilhydrazil) and FRAP (ferric reducing antioxidant power). Kersen leaves extracted by maceration using ethanol 70%. Phytochemical identification of kersen leaves contain phenolic, flavonoids and saponins compounds. The test results of antioxidant activity using DPPH method with series concentrations of 2, 4, 6, 8, and 10 ppm obtain IC50 value 6.8249 ug / ml. Testing of the ethanol extract of antioxidants obtained by FRAP method IC50 83,149 µM. Based on these data the ethanol extract of kersen leaves has good antioxidant activity.
Sintesis Natrium Karboksimetil Selulosa (Na.CMC) dari Selulosa Hasil Isolasi dari Batang Alang-Alang (Imperata cylindrica L.) Megawati, Megawati; Sami, Fitriyanti Jumaetri; Syatriani, Syatriani
Jurnal Farmasi dan Ilmu Pengobatan Vol 2 No 1 (2017): JPMS
Publisher : STIFA Makassar

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Abstract

Alang-alang (Imperata cylindrica L) is a plant thatis used as fodder. This plant contain a high cellulose content 96,7%, which can be used as the manufacture of sodium carboxymethyl cellulose. The purpose of this study to synthesize the carboxymethyl cellulose from the isolation of coarse grass (Imperata cylindrica L). Na.CMC  of coarse grass (Imperata cylindrica L) obtained by alkalization of cellulose with sodium hydroxide in the solvent isopropyl alcohol in submitting etherification using sodium chloro acetate. Results of qualitative testing color identification with reactant molischshowed positive resuls, analysis formed muchilagform, a o white swelling, viscocity 8Cp, pH of 6 with a purity of 95.8%, flame test Na+ are marked with a yellow flame and  FTIR results showed the wave number 3442 cm-1 -OH group, 2918 cm-1 CH , 1647 cm-1 group C = O, 1265cm-1 CO group and 894 cm-1 is CH that is the Na.CMC the synthesis.
UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH Sami, Fitriyanti Jumaetri; Soekamto, Nunuk Hariani; Firdaus, Firdaus; Latip, Jalifah
Jurnal Kimia Riset Vol 4, No 1 (2019): Juni
Publisher : Universitas Airlangga

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Abstract

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of  T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL. 
Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan Nur, Syamsu; Sami, Fitriyanti Jumaetri; Awaluddin, Akbar; Afsari, Mutiara Indah Ayu
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Vol 5, No 1 (2019): (March 2019)
Publisher : Universitas Tadulako

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Abstract

This experiment aims to determine the correlation of total phenolic and flavonoid content of jati putih leaves fraction (Gmelina arborea Roxb.) towards Antioxidant activity .  Sample was extracted by maceration method using ethanol 70% to obtain the ethanol extract (EE), followed by liquid-liquid extraction method to obtain fraction of ethyl acetate (EA) and n-Hexane (EH). The phytochemical screening  and determination of total phenolic and flavonoid content were done by colorimetric method. Antioxidant activity were done by DPPH, FRAP and ABTS methods. Phytochemical screening showed positive results for flavonoids, phenolic and saponins.  The largest total phenolic content was found on EA (11,59 µg/ml ± 0,3 %b/b EAG) and the largest total flavonoid content was on EA (3,88 µg/ml ± 0,02 %b/b EK). The total phenolic and flavonoid content of Jati putih leaves has a correlation with antioxidant activity. The coefficient correlation of activity on reducingDPPH radical was 56,7% (total of phenolic content) and 57,8% (total of flavonoid content) and on iron reduction power in FRAP method  was 99,9% (total of phenolics and flavonoids content). The relationship with the activity in reducing radical ABTS obtained coefficient correlation of 57,0% and 58,1% for total phenolic and flavonoids contents, respectively.