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The Effect of Green Tea Leaf Extract on Spatial Memory Function and Superoxyde Dismutase Enzyme Activity in Mice with D-galactose Induced Dimentia

Sains Medika Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Introduction: Oxidative stress and inflammation play an important role in pathogenesis of brain aging and neurodegenerative diseases such as Alzheimer. Green tea has been shown to have antioxidant, anti-inflammatory, anticancer, and neuroprotective activity. Objective: to determine the effect of green tea extract on spatial memory function and superoxide dismutase enzyme activity in mice with D-galactose induced dementiaMethods: An experimental study using “post test only control group design”. Twenty male BALB/c Mice aged 6-8 weeks were divided into 4 groups. Negative control group (NG) was induced by subcutaneous injection of D-galactose (150 mg/kg BW) once daily for 6 weeks. GT-90, GT-270, GT-540 were induced by D-galactose and orally administered with 90, 270, and 540 mg/kg BW of green tea extract once daily for 6 weeks. The spatial memory functions were assessed using Morris water maze and SOD enzyme activities were evaluated using ELISA. One-way Anova and Kruskal-Wallis were used for statistical analysis.Results: mean percentage of latency time in the GT-90 (35.29 (SD= 2.69)%), GT-270 (35.28 (SD= 2.62)%), and GT-540 (35.62 (SD=5.05)%) were significantly higher compared to that of NG (20.38 (SD = 3.21)%), p <0.05). SOD enzyme activity in the GT-270 (0.78 (SD = 0.07) U/ml) was significantly higher compared to that of NG (0.51 (SD = 0.01) U ml), p= 0.004).Conclusion: Green tea extract may improve spatial memory function and the activity of superoxide dismutase enzyme in mice with D-galactose induced dementia.

PERBANDINGAN KUALITAS TENAGA KESEHATAN PADA PELAYANAN DIARE ANAK DI LAYANAN PRIMER (ANALISA DATA INDONESIAN FAMILY LIFE SURVEY (IFLS) 2007)

Media Medika Muda Vol 2, No 1 (2017)
Publisher : Faculty of Medicine Diponegoro University

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Abstract

Latar Belakang: Diare merupakan salah satu penyakit yang berpengaruh terhadap kematian anak. Kualitas tenaga kesehatan yang baik dapat berpengaruh positif terhadap kualitas pelayanan. Pelayanan primer memiliki peranan yang penting untuk meningkatkan derajat kesehatan. Penelitian ini bertujuan untuk mengetahui perbandingan kualitas tenaga kesehatan (dokter, perawat dan bidan) pada pelayanan primer di Indonesia.Metode: Penelitian kuantitatif menggunakan analisis data sekunder Indonesian Family Life Survey (IFLS) 2007 dengan unit analisis tenaga kesehatan dan desain cross sectional. Data IFLS 2007 diambil dari 13 provinsi. Perbandingan kualitas tenaga kesehatan dinilai dari pertanyaan vignette meliputi anamnesis, pemeriksaan fisik, pemeriksaan laboratorium dan terapi pada blok puskesmas dan fasilitas kesehatan praktek swasta. Kemudian di uji menggunakan one way-Annova dengan program Stata 12 dengan kemaknaan p < 0.05.Hasil :Sampel terdiri atas 2179 tenaga kesehatan. Dokter memiliki rerata tertinggi dalam menjawab seluruh pertanyaan yaitu sebanyak22.25±7.9 dari 56 pertanyaan.Dari 26 pertanyaan anamnesis, rerata dokter menjawab 11±4.1 lebih tinggi dibandingkan dengan perawat : 9.06±3.3 dan bidan : 10.07 ±3,7 (p<0.05). Dari 19 pertanyaan pemeriksaan fisik dan laboratorium, rerata dokter menjawab7.62±3.1, lebih tinggi dari perawat 6.36±2.9 dan bidan 6.89±2.9 (p < 0.05).Dari 19 pertanyaan terapi rerata dokter 3.62±1.8, tidak berbeda secara statistik dibanding bidan 3.64±1.7 (p>0.05).Simpulan : Dokter memiliki kualitas lebih baik dibandingkan dengan perawat dan bidan dalam kemampuan anamnesis, pemeriksaan fisik – laboratorium dan kemampuan dalam menjawab seluruh pertanyaan. Kemampuan Dokter tidak berbeda dibandingkan bidan dalam memberikan terapi diare. Kata kunci: diare, perbandingan, kualitas tenaga kesehatan, IFLS

The Effects of Zinc Supplementation on Immune Response of Obese Children With Family History of Atopy

Journal of Biomedicine and Translational Research Vol 4, No 2 (2018): December 2018
Publisher : Faculty of Medicine, Diponegoro University

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Abstract

Introduction: Obesity is associated with low-grade chronic inflammation and disbalance between cytokine production of T helper1 and T helper2 cells.  Zinc plays role in balancing immune response, has anti-oxidant and anti-inflammation activities; its deficiency also common in obesity.  The role of zinc supplementation in children with obesity with risk of atopy needs to be elucidated.Materials and Method: We performed randomized controlled trial double-blind to investigate the effect of daily 15 mg zinc supplementation for 6 weeks in children aged 13 to 14 years old with obese who has family history of atopy.  Subjects were divided into group of zinc supplementation and placebo, recruited from junior high schools from Semarang municipality, performed during periods from January to December 2013.  Plasma zinc concentration, leptin, immunoglobulin-E (Ig-E), interferon-γ (IFN-γ), interleukin-4 (IL-4) were measured, also body weight, height and ISAAC questionnaire to recruit subjects with family history of atopy.Result: There were 36 subjects.  The concentration of zinc serum, Ig-E, IFN-γ, and IL-4 of two groups before supplementation were not significantly different (p>0.05).  Zinc supplementation increase the mean level of zinc concentration on treatment group compare to placebo group (27.74+11.80 vs 3.47+2.55, p=0.000).  The concentration of Ig-E, IFN-γ and IL-4 serum were decreased at treatment group (15.5+20.46 vs 20.02+38.02, p=0.613), (1.82+3.59 vs 0.911+3.51, p=0.174), and (4.76+6.49 vs 2.44+4.41, p=0.085) respectively.  Conclusion: Zinc supplementation increased the level of zinc serum concentration of obese children and decreased the risk of atopy.

EFEK PEMBERIAN EKSTRAK DAUN Carica pubescens TERHADAP JUMLAH LEUKOSIT PADA TIKUS Sprague Dawley YANG DIINDUKSI AZOXYMETHANE : STUDI DI LABORATORIUM PENELITIAN DAN PENGUJIAN TERPADU 4 UNIVERSITAS GADJAH MADA

JURNAL KEDOKTERAN DIPONEGORO Vol 8, No 1 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Program Studi Kedokteran Umum, Fakultas Kedokteran, Universitas Diponegoro

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Abstract

Latar belakang: Inflamasi adalah salah satu faktor penyebab terjadinya kanker kolorektal. Flavonoid adalah suatu senyawa yang terkandung dalam daun Carica pubescens diyakini memiliki efek antiinflamasi. Tujuan: mengetahui efek pemberian ekstrak daun Carica pubescens terhadap jumlah leukosit pada tikus Sprague dawley yang diinduksi azoxymethane. Metode: jenis penelitian ini adalah post test only control group design dengan sampel terdiri dari 25 tikus Sprague dawley yang dibagi dalam 5 kelompok yaitu K1 diberi injeksi NaCl 0,9% seminggu sekali selama dua minggu, K2 diberi injeksi azoxymethane seminggu sekali selama dua minggu. P1, P2 dan P3 yang diberi injeksi azoxymethane seminggu sekali dalam dua minggu dan diberi ekstrak daun Carica pubescens dengan dosis 100 mg/KgBB, 200 mg/KgBB dan 400 mg/KgBB. Jumlah leukosit dihitung dengan alat hematology analyzer Sysmex KX-21 yang dinyatakan dalam sel/μL. Data dianalisis dengan One way Anova dan uji Post Hoc. Hasil: rerata jumlah leukosit kelompok K2 (7000 ± 2065,2 μL) lebih tinggi signifikan dibanding K1(4830 ± 449,4 μL, p=0,002) dan P1 (4380 ± 715,5 μL, p=0,002). Rerata kelompok K2 (7000 ± 2065,2 μL) lebih tinggi namun tidak berbeda signifikan dengan kelompok P2 (6820 ± 1030,5 μL, p=0,807) dan P3 (6020 ± 759,6 μL, p=0,193). Kesimpulan: ekstrak daun Carica pubescens menurunkan jumlah leukosit.Kata Kunci: Carica pubescens, leukosit, kanker kolorektal.