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PENINGKATAN PRODUKSI SEFALOSPORIN C DARI Acremonium chrysogenum CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN Prabandari, Erwahyuni; Hidayati, Dyah Noor; Dewi, Diana; Islamiati, Eni Dwi; Syamsu, Khaswar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 4, No 1 (2017): June 2017
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v4i1.1808

Abstract

Cephalosporin is a β-lactam antibiotic produced by Acremonium chrysogenum using submerged fermentation. Carbon and nitrogen are the most influential medium ingredients for cephalosporin formation. The purpose of this study was to obtain the best composition of media for cephalosporin C production. Response surface methodology was used for production optimization. The results showed that molasses of 70 g/Lwas the best carbon source, while the best nitrogen source was the combination of corn steep liquor, urea and ammonium sulphate. DL-methionine, carbon, and nitrogen source significantly affected  the production of cephalosporin C. The mathematically modelled optimization showed that the highest production of cephalosporin C (3876 mg/L) was obtained using medium composition of 68.28 g/L molasses, 71.61 g/L nitrogen, and 0.4 g/L DL-methionine. Laboratory verification using the same medium composition produced 3696 mg/L of cephalosporin C, being 4.65% different from the mathematically optimized results. Medium optimization increased the cephalosprin C production which was 1.48 times higher than that using the previous medium, where the maximum production was only 2487 mg / L.Keywords: Carbon,  cephalosporin C, cultivation medium, nitrogen, A. chrysogenum ABSTRAKSefalosporin C adalah golongan antibiotik β-lactam yang dihasilkan Acremonium chrysogenum melalui fermentasi cair. Komponen yang sangat berpengaruh terhadap produksi sefalosporin C adalah sumber karbon dan nitrogen. Penelitian ini bertujuan mendapatkan komposisi media terbaik untuk produksi sefalosporin C. Optimasi dilakukan menggunakan metode respon permukaan. Hasil menunjukkan bahwa molases 70 g/L adalah sumber karbon terbaik dan kombinasi corn steep liquor, urea dan ammonium sulfat adalah sumber nitrogen terbaik. DL-methionin, sumber karbon, dan nitrogen berpengaruh nyata terhadap produksi sefalosporin C. Optimasi menggunakan model matematika menunjukkan produksi sefalosporin C tertinggi (3876 mg/L) yang diperoleh dengan komposisi media 68,28 g/L molases, 71,61 g/L nitrogen, dan  0,4 g/L DL-methionin. Verfikasi di laboratorium menggunakan komposisi media yang sama menghasilkan sefalosporin C sebesar 3696 mg/L, berbeda 4,65% dibanding dengan hasil optimasi matematis. Optimasi media mampu meningkatkan produksi sefalosprin C sebesar 1,48 kali dibanding media yang digunakan sebelumnya, dimana maksimal hanya menghasilkan 2487 mg/L.Kata kunci: Karbon, sefalosporin C, media kultivasi, nitrogen, A. chrysogenum
PENGARUH BEBAN KERJA DAN LINGKUNGAN KERJA TERHADAP KINERJA KARYAWAN PADA PT. AN-NUR KANGGRAKSAN CIREBON Kartikasari, Wiwit; Dewi, Diana
VALUE Vol 12 No 2 (2017): Edisi : Juli - Desember 2017
Publisher : Prodi Ilmu Manajemen, Fakultas Ekonomi Universitas Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Problems in this research is the workload given by PT. An-Nur Kanggraksan Cirebon to many employees that burdensome employees so that the work is completed with extra time (overtime), while the workplace environment is felt by employees is still less comfortable resulting in decreased even though not significantly reduced employee performance. This study aims: 1) To describe data about the effect of workload on employee performance PT. An-Nur Kanggraksan Cirebon. 2) To describe data about the influence of work environment on employee performance PT. An-Nur Kanggraksan Cirebon. 3) To describe data about the influence of workload and work environment simultaneously on the performance of employees of PT. An-Nur Kanggraksan Cirebon. The data collecting of this research is done through questionnaire to 50 employees as the research sample. While the data processing using statistical data analysis using multiple linier regression analysis method. The conclusions of this study are: 1) Work load affect the performance of employees at PT. An-Nur Kanggraksan Cirebon based on the results of data analysis is known to prove at the level of significance of ? = 0.05 (5%) ie with a tcount of 2.239> ttable of 1.998 meaning significant. Thus if the workload is increasing then the performance of employees participate in the increase. 2) Work environment affect on employee performance at PT. An-Nur Kanggraksan Cirebon based on the results of data analysis is known to prove at the level of significance of ? = 0.05 (5%) ie with a t count of 2.184> ttable of 1.998 meaning significant. Thus the improved and conducive work environment hence increasing the performance of employees. 3) Meanwhile, workload and work environment are proven jointly affect the performance of employees at PT. An-Nur Kanggraksan Cirebon based on the results of data analysis known this result proved to be believed at the level of significance of ? = 0.05 (5%) with the value of Fhitung 6.187> Ftable of 3.14 means significant. If the workload and work environment are synergized in improving employee performance then the result obtained is the influence of workload and work environment on employee performance is quite meaningful.   Key words : workload, work environment and employee performance.
Analisis Promosi Aspictura Photography Melalui Media Sosial Instagram Dewi, Diana; Widayatmoko, Widayatmoko
Prologia Vol 1, No 2 (2017): Prologia
Publisher : Fakultas Ilmu Komunikasi Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/pr.v1i2.1951

Abstract

Fotografi merupakan salah satu bisnis di bidang pernikahan yang sudah menjadi kebutuhansetiap pasangan yang ingin melangsungkan pernikahannya, yaitu untuk mengabadikan momen-momen berharga pada saat itu. Dalam menjalankan sebuah bisnis, tentunya diperlukan kegiatan promosi. Pada era modern ini, promosi tidak hanya dapat dilakukan melalui media konvensional, namun juga melalui media baru, seperti internet. Pada penelitian ini, penulis membahas mengenai Aspictura Photography yang melakukan promosi melalui salah satu media yang merupakan perkembangan dari internet, yaitu media sosial Instagram. Tujuan penelitian ini adalah untuk mengetahui promosi yang dilakukan oleh Aspictura Photography melalui Instagram. Teori yang digunakan pada penelitian ini adalah promosi dan media sosial. Penelitian ini menggunakan metode penelitian kualitatif deskriptif. Subyek dari penelitian ini adalah Aspictura Photography, dan obyek dari penelitian ini adalah promosi. Data penelitian ini diperoleh melalui wawancara dengan pemilik dan head of marketing dari Aspictura Photography. Promosi yang dilakukan oleh Aspictura Photography mencakup iklan, promosi penjualan, hubungan masyarakat, penjualan personal, pemasaran langsung, dan pemasaran interaktif. Pemilihan Instagram sebagai media promosi sudah tepat, dan fitur-fitur yang terdapat di Instagram memudahkan Aspictura Photography dalam melakukan promosi dan menyebarkan informasi.
The Illocutionary Acts of Environmental Persuasion in U.S. Online Newspaper Opinion Articles Dewi, Diana
Beyond Words Vol 7, No 2 (2019)
Publisher : Widya Mandala Catholic University Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.33508/bw.v7i2.2151

Abstract

Persuasion, especially in written form, has been acknowledged by scholars as difficult. Success in its attempt requires complex use of, among others, pragmatics. Persuasive texts have therefore been highly researched using the speech act theory. However, a research gap existed for a study of illocutionary acts in newspaper opinion articles. To fill the gap, the current study did a qualitative textual analysis on 10 U.S. online newspaper opinion articles that talk about climate change. The analysis was conducted to discover types of illocutionary acts and persuasive strategies the writers use to successfully persuade readers. It was then found that the writers predominantly performed assertives. The second most frequently used were directives. The most preferred subtypes under these categories were, among others, informing, urging, assuring, and demanding. These preferences further revealed the writers? persuasive strategies. The strategies were as follows: being highly implicit, being occasionally explicit, giving more information about the issue, conveying certainty and uncertainty, arousing fear, also creating a sense of urgency. These acts and strategies, according to existing theories, could increase the texts? persuasiveness. Hence, this study establishes that when used in persuasive texts, the previously mentioned illocutionary acts and strategies can help writers to attain persuasion.