Muhammad Cahyadi, Muhammad
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Published : 4 Documents
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Produksi dan Kualitas Susu Sapi Perah Penderita Mastitis yang Mendapat Pengobatan Antibiotik

Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 14, No 2 (2016): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

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Abstract

This study aims to determine differences of production, the physical quality and chemical quality of dairy cattle mastitis patients before and after treatment with antibiotics penicillin-streptomycin. This research was conducted in farmer group (KTT) Subur Makmur, Banyuanyar Village, District Ampel, Boyolali. The material used is 15 cows PFH suffering from mastitis in lactation period 3rd trimester, aged 3 to 5 years. Combinations of antibiotics used is penicillin G 3 grams of mixed powder injection streptomycin sulfate injection of 1 gram powder is then dissolved with aquadestilata until volume of 15 ml. Antibiotics do 1 times a day for 5 days intramuscularly and intramammae. This research was carried out experimentally with two treatments and 15 replications. Data were analyzed by paired sample t-test (paired sample t-test). The analysis showed highly significant (P<0,01) on milk production, protein content, lactose, fat, nonfat dry (BKTL) and the Californian Mastitis Test (CMT), significant differences (P<0,05) pH, color, smell and consistency as well as the difference was not significant (P>0,05) against density. The conclusion of this research is the treatment of penicillin-streptomycin antibiotic use in dairy cows mastitis patients effectively improve the production, chemical quality and physical qualities but can not improve the density of milk. Keywords: mastitis, production, physical quality, chemical and bacteriological quality, milk, penicillin, streptomycin

AUTENTIKASI DAGING AYAM SEGAR DARI KONTAMINASI DAGING BABI MENGGUNAKAN GEN CYT-B DENGAN ANALISIS DUPLEX- POLYMERASE CHAIN REACTION

Buletin Peternakan Vol 41, No 2 (2017): BULETIN PETERNAKAN VOL. 41 (2) MEI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

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Abstract

Halal is one of important aspects in consumer protection. Meat and processed meat products are food that should be controlled strictly because those are prone to be adulterated by pork contamination. Therefore, it is necessary to provide detection technique which is accurate, fast and cheap. The objective of this research was to identify the presence of impurities of pork meat on raw chicken meat using gene Cyt-b with duplex-PCR analysis. This research used six samples of raw chicken meat and raw pork. Raw chicken meat was bought from supermarkets in the city of Surakarta and raw pork was obtained from pig slaughterhouse. The percentage of raw pork contamination on raw chicken meat was designed as much as 1, 5, 10, and 25%, respectively. The DNA genome was isolated according to DNA isolation protocol from Genomic DNA Mini Kit. In addition, duplex-PCR was performed based on protocol of KAPA2G Fast Multiplex PCR kit. The data was descriptively analyzed by directly looking the DNA bands on the gel documentation apparatus. The result showed that specific DNA bands for chicken and pig were completely appeared on 1.5% of agarose gels. Duplex-PCR detect contamination of pork on raw meat of chicken at all contamination levels. This research proved that the duplex-PCR detect the contamination of pork until the level of 1%.

The Effect of Various Decomposers on Quality of Cattle Dung Compost

Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

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Abstract

The aim of this study was to compare the utilization of decomposer from bioethanol waste (DLB) with various commercial decomposers on quality of compost made from cattle dung. This study used completely randomized design (one-way ANOVA) with four treatments and five replications. Materials used in this study were cattle dung, water, molasses, DLB and various commercial decomposers (A, B, and C). The treatments in this study were cattle dung + BLB 0.5% (P0), cattle dung + decomposer A 0.5% (P1), cattle dung + decomposer B 0.5% (P2) and cattle dung + decomposer C 0.5% (P3). Then, the mixtures were aerobically ripened until four weeks. During composting, compost was stirred weekly. Furthermore, mature compost was analyzed to evaluate the amount of C Organic, total N, total P, total K, total Ca, total Mg, C/N ratio and pH value. The analysis of variance showed that there was no difference of the quality and pH value of compost made from cattle dung with utilization of DLB and commercial decomposers. It could be concluded that utilization of DLB in composting of cattle dung had identically quality and pH value of compost with utilization of commercial decomposers, therefore, this technology could be an alternative to process waste from home industry of bioethanol.

Komposisi Unsur Hara Kompos yang Dibuat dengan Bantuan Agen Dekomposer Limbah Bioetanol pada Level yang Berbeda

Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 16, No 2 (2018): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

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Abstract

The objective of research was to evaluate the effect of different level of decomposers from bioethanol waste as a bioactivator to compost nutrient content. The materials used in this study were feces collected from six 3 to 5 years old of male Fresian Holstein (PFH), and decomposer agents from processed-bioethanol waste. The experimental design used was completely randomized design with four treatments and six replications, i.e. P1: 0.4% of decomposer, P2: 0.5% of decomposer, P3: 0.6% of decomposer, P4: 0.7 of decomposer %. Analysis of nutrient contents of compost was conducted at the the Laboratory of Chemistry and Soil Fertility, Department of Soil Science, Faculty of Agriculture, Gajah Mada University, Yogyakarta. Data analysis was performed using Minitab 17 software. The results of the analysis of variance showed that different levels of decomposer affected organic C, phosphor (P), Kalium (K), and Magnesium (Mg). Organic C, P, K, and Mg contents were found highest in P2, on the other hand N and Ca contents, C/N ratio, pH, and temperature of compost were not different among treatments. It could be concluded that compost made with 0.5% decomposer agents from processed-bioethanol waste was the best.