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UJI EFEKTIVITAS ASAP CAIR CANGKANG BUAH Hevea braziliensis TERHADAP AKTIVITAS BAKTERI Escherichia coli

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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Abstract

This research purpose to determine the effectiveness of the liquid smoke of the Hevea braziliensis  fruit shell  against the activity of Escherichia coli bacteria. The preparation and purification of liquid smoke is carried out by 4 stages: pyrolysis , sedimentation, stage 1 and stage  2  distillation. This research was started with total acidity test, phenol level, pH and density with result of measurement 4,725% total acid content, 0,84% phenol content, 2,548 pH and 1,004 in density . An antibacterial test  using disc diffusion method by looking at the diameter of the clear zone. There are six concentrations of liquid smoke of  H.braziliensis  rubber shell used, negative control using aquades and positive control using ciprofloxacin . An  effective concentration was found in inhibiting E.coli is 100% concentration with a clear zone diameter of 11.3 mm. The result of analysis using One Way ANOVA showed F count (4.42)> F table (3,23), then H0 rejected, meaning there is a significant difference of influence of concentration to the diameter of clear zone produced.

AKTIVITAS ASAP CAIR CANGKANG BUAH Hevea braziliensis SEBAGAI ANTI BAKTERI Staphylacoccus aureus

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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Abstract

The aims of this research is to know the activity of liquid smoke Hevea braziliensis  Seed Shell as antibacterial of Staphylacoccus aureus. The synthesis of  liquid smoke on this research through some process such as pyrolysis, sedimentation, filtration, distillation and redistillation. H.braziliensis Seed Shell liquid smoke  obtained phenol levels 0.84% and pH  2.548. Antibacterial activity tested by hollow difusion methode with variations concentration of liquid smoke .at 5%, , 10 , 25 , 50, 75, and 100 % v/v. The effective concentration H.braziliensis  liquid smoke in inhibiting the growth of S. aureus bacteria is 75% v/v with strong antibacterial level that showed by an inhibition zone at 10,6 mm. On this research One Way ANOVA test showing the Fcount   ? F table, which means there is a significant difference between the diameter of the inhibition zone on various concentrations of H.braziliensis seed shell.iquid smoke..

MENGGUNAKAN MEDIA INTERNET DOCS.GOOGLE PADA POKOK BAHASAN REAKSI REDOKS KELAS X IPA DI SMAN 4 KOTA BENGKULU

Alotrop Vol 2, No 1 (2018)
Publisher : FKIP University of Bengkulu

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[USING THE DOCS. GOOGLE INTERNET MEDIA ON THE REDOX REACTION  SUBJECT IN CLASS X IPA SMAN 4 BENGKULU CITY  ] This research aims to know the results of learning: students in the class who are not given the preparation of learning at home, knowing the results of the teaching of students in a given grade of preparatory study at home using internet media, knowing the Docs. Google the difference in the results of student learning in the classroom are not given preparation home study with the results of a survey of students in a provided a homework preparatory sign Docs. Google internet media. The methods used in this research was quasi-experimental research is the entire population with grade X IPA SMAN 4 city of Bengkulu, Sample research, i.e., class experiments and classroom control. The results of the study in this research result of students pretest and posttest. Based on the results of the research conducted in mind that there is a significant difference between the results from student learning in the classroom are not given preparation home study with the results of a survey of students in a given a homework preparation home study using internet media Docs. Google via t-tests using SPSS 16 program. The learning outcomes of students in a given grade of preparatory education at home using Google Docs. Indicates the average rating of 77.0 while learning results of students in the class who are not given a homework preliminary with the average value of 68.6. Student Learning Outcomes in a given class homework preparation using Docs. Google may provide better results on the subject of redox reactions.

POTENSI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI BAKTERI ENDOFIT PADA DAUN Moringa oleifera L

Alotrop Vol 2, No 1 (2018)
Publisher : FKIP University of Bengkulu

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[POTENTIAL ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITES FROM ENDOPHYTE BACTERIA ON Moringa oleifera L LEAF] This research aims to know the similarities between secondary compound metabolites produced by endophyte bacterial on host leaves and from  Moringa oleifera L (kelor) leaves as well as determine the magnitude of antioxidant activity compounds of secondary metabolites produced by bacterial endophyte. Based on the results of the test compound is secondary metabolite in both samples, for secondary metabolite compounds in the extract of the leaves of M. oleifera L. contains identified steroid compounds, flavonoids, saponins, tannins and phenolic compounds, while for metabolites secondary bacterial endophyte undetectable on the existence of any secondary metabolite compounds.For testing of antioxidants on endophyte bacterial extracts of leaves of M. oleifera L is done using the DPPH method, performed on variations of the concentration of 200, 400, 600, 800, and 1000 ppm with Ascorbic acid as a comparison. The results of the test get an IC50 of M. oleifera L leaf extract at 243.67 ppm, and supernatan extract from endophyte bacterial at 492 ppm. The IC50 values showed antioxidant activity in secondary metabolite compounds derived from extracts of the leaves of M. oleifera L which has an extreme antioxidant category. The amount of antioxidant activity for secondary metabolite compound extracts of bacterial endophyte on leaves of M. oleifera L category is feeble, and to extract the supernatan activity is weak compared to the value of the antioxidant activity of Ascorbic acid.

Studi Komponen Kimia Pelepah Sawit Varietas Tenera Dan Pengembangannya Sebagai Modul Pembelajaran Kimia

PendIPA Journal of Science Education Vol 1, No 1 (2017): october
Publisher : University of Bengkulu

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This study aims to (1) determine the levels of the components of the Tenera variety of palm oil compounds including extractives, holocellulose, ? - cellulose, and lignin. (2) Analyze the utilization of the pulp of Tenera varieties as pulp raw materials based on their chemical components; (3) application of chemistry learning module to improving student learning outcomes. Determination of extractive substance content with TAPPI test methods Q: 204; lignin content T: 222; holocellulose Q: 9 levels and ?-cellulose content with TAPPI test methods T: 204. then lignin, holocellulose and ?-cellulose produced from the procedure were characterized by an IR spectrophotometer. The results of the study were module and implemented in ICHO students in SMAN 2 Kota Bengkulu. The data of the research results were analyzed by ANOVA test at 5% level. The results of the characterization of lignin, holocellulose and ?-cellulose with FTIR obtained a distinctive peak of the respective functional groups of the macromolecules. From the research also obtained the average value of chemical component content on the palm velvet varieties of tenera according to their part of base, middle, ends with mean for extractive substance 8.49%, 7,87%, 6,74%, lignin 20,7 %, 18.95%, 16.69%. holocellulose 81.57%, 80.33%, 79.24% and ?- cellulose 44.57%, 43.56%, 43.26%. Based on the results of the variance analysis, the difference in position (base, center, tip) on the palm oil of the tenera varieties on extractive, lignin, holocellulose and ?-cellulose substances has significant differences. Based on the chemical component classification of Indonesian wide wood leaf, sheep betera varieties of tenera in all three positions are used as pulp raw materials because they have moderate lignin content, high levels of Holocellulose and moderate levels of ?-cellulose. The result of module implementation in students there is a significant difference between pretest and posttest value. The use of modules in learning in science groups can improve student learning outcomes.

Penjerapan Zat Warna Sintetis Menggunakan Karbon Aktif Kelapa Sawit Dan Pengembangannya Sebagai Bahan Ajar

PendIPA Journal of Science Education Vol 1, No 1 (2017): october
Publisher : University of Bengkulu

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This study aims to: (1) explain the ability of the active carbon absorption of Palm Oil Palm as a dye adsorbent Reactive Red and Direct Green (2) determines the pH, contact time, and weight of the optimum adsorbent and the influence of temperature using activated carbon. Reactive Red and Direct Green dyestuffs. (3) to explain the influence of video media usage on the improvement of learning result and critical thinking ability of students in Chemistry class XI MIPA E at SMAN 2 Kota Bengkulu. The procedure in this study is the activation of activated carbon of palm oil and activated, activated carbon characterization using FTIR, Determination of maximum wavelength (?), Calibration curve creation, Adsorption of substance by activated carbon of palm oil: determining pH, contact time, adsorbent weight, , adsorption isotherms, Determination of temperature effect, Adsorption Isotherm Determination, Determination of adsorption kinetics, Applications on dyestuffs. The data were analyzed. The result of pH variation, variation of adsorbent weight, and contact time were made curve so that pH, adsorbent weight, concentration and optimum contact time were obtained using Excel program. Using ANAVA one way, followed by KR-20 test and one sample t test. The results showed that there were: reactive red optimum wavelength 496 nm, optimum pH 3, optimum time 40 min, absorbent weight 150 mg, maximum absorbency KAPKS 32,73 mg / gr and direct green optimum wavelength 613 nm, with optimum pH 5, optimum time 40 minutes, absorbent weight 150 mg, maximum absorbency KAPKS 32.825 mg / gr. there is an increase of chemistry learning outcomes during the enrichment hours or outside of class hours and critical thinking skills of grade XI IPA E SMAN2 Kota Bengkulu using audio-visual media with average pretest score of 75 and average post test score 82.27 with four criteria critical thinking skills are less critical by 26%, critical enough 43%, critical 23% and very critical at 8%.

Pengembangan media pembelajaran e-book berdasarkan hasil riset elektroforesis 2-d untuk mengukur kemampuan berpikir kreatif mahasiswa

PendIPA Journal of Science Education Vol 2, No 2 (2018): june
Publisher : PendIPA Journal of Science Education

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ABSTRAKPenelitian ini bertujuan untuk mengemas hasil profil protein hati Mus musculus dengan elektroforesis dua dimensi ke dalam buku elektronik (e-book), dan mengetahui kemampuan berpikir kreatif  mahasiswa terhadap implementasi pembelajaran menggunakan media e-book. Metode yang digunakan dalam penelitian ini adalah penelitian pengembangan (Development Research). Hasil penelitian ini  yaitu media pembelajaran e-book yang dibangun berdasarkan hasil riset secara struktural terdiri dari; a) pendahuluan, b) materi isi elektroforesis, c) analisis hasil penelitian,  d) kesimpulan, e) evaluasi, dan f) biodata penulis. Setelah e-book diimplementasi terhadap mahasiswa sebanyak 30 orang menunjukkan bahwa mahasiswa yang berpikir sangat kreatif berjumlah 19 orang (63%), mahasiswa berpikir kreatif 7 orang (23,3%), dan mahasiswa berpikir cukup kreatif 3 orang (13,3%).Kata kunci : E-book ; elektroforesis 2-D; kemampuan berpikir kreatif.

KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT

Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis 

UJI AKTIVITAS ASAP CAIR CANGKANG BUAH KARET (Hevea brassiliensis) DAN APLIKASINYA DALAM PENGHAMBATAN KETENGIKAN DAGING SAPI

Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

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This research was aimed to know the degree of Fenol compound, total acid and the activity of liquid smoke of Rubber (Hevea brassiliensis) Seed Shell also the application in obstructing the rancidity of beef. The Fenol compound was determined by using Reagen Follon-Ciocalteu. The total acid was determined by using the method of titrated acids.The ability as the antioxidant was tested by DPPH method and the ability of obstructing the rancidity of beef was done by measuring the alteration of TBA value in beef as long as saved. The result was shown the fenol compound in liquid smoke of Rubber Seed Shell as much as 0,84 % and the acetic acid content was 4.725%. The antioxidant test was produced IC50 value as much as 101.27 ppm. According to the IC50 value which got from the test that the liquid smoke of Rubber Seed Shell were included in moderate category. The increment of the liquid smoke of Rubber Seed Shell were able to suppress the rancidity of beef during the storage time. It was marked by the decreasing of TBA value in beef as given in treatment A1 (liquid smoke 4% ) and A2 liquid smoke 6%. The treatment without the liquid smoke (A0) increased the TBA value from 0.05 mgMDA/Kg on day (0) to 0.615 mgMDA/Kg on day 6. The treatment of concentration liquid smoke 4% (A1)  increased  from  0.039  mgMDA/Kg  on  day  (0) to  0.395  mgMDA/Kg  on  day  6.  The  treatment  of concentration liquid smoke 6% (A2) increased from 0.031 mgMDA/Kg on day 0 to 0.209 mgMDA/Kg on day 6.

PERBANDINGAN AKTIVITAS DAN HASIL BELAJAR KIMIA SISWA SMAN 2 ARGAMAKMUR PADA PEMBELAJARAN MENGGUNAKAN TEAM ASSISTED INDIVIDUALIZATION DAN GROUP INVESTIGATION BERBASIS KURIKULUM 2013

Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

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This research was aimed to know  the differences between the student’s chemical learning activities and achievement by using a models called Team Assisted Individualization and Group Investigation based on Curriculum 2013. The samples in this research is class X MIA 3 (Team Assisted Individualization) and X MIA 6 (Group Investigation). The result of this research is learning activities and achievement and than the analysis used are the score and percentage of mean, normality tests, homogeneity tests, hypothesis tests. The observation result of student’s learning activities in the 1 and 2 of experimental class was 78.79% and 68,18%. The mean score of student’s achievement for experimental class 1 and 2 was 80,56 and 75,31. The result of t test obtained tcount > ttable, 10,6 > 1,0 for learning activities and 3,36 > 2,30 for achievement. It means there was a significant differences between the student’s chemical learning activities and achievement by using both models of learning.