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Pewarnaan Gram Buffy Coat untuk Deteksi Awal Pasien Bakteremia lutpiatina, leka
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity. Keywords: Gram stain, Buffy Coat, bacteraemia Abstrak: Bakteremia adalah salah satu masalah kesehatan masyarakat. Bakteremia menempati peringkat kesepuluh sebagai penyebab utama kematian di Amerika Serikat (Kung H et.al., 2008). Pewarnaan Gram dari buffy coat dapat digunakan untuk deteksi awal pasien bakteremia. Tes diagnostik yang cepat dengan pewarnaan Gram ini dapat di interpretasikan dalam waktu satu jam dan dalam penelitian yang telah dilakukan Richmond et al. 2002, mempunyai sensitifitas 75% dan spesifisitas 79%. Penelitian ini dimaksudkan untuk mempelajari sensitifitas dan spesifisitas pewarnaan Gram buffy coat dengan gold standar kultur darah media bifasik pada pasien tersangka demam. Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional study. Populasi penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013. Sampel penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013 yang diambil secara purposive dengan Kriteria inklusi : Pasien Rumah Sakit Umum Daerah Ratu Zalecha Martapura, berumur ≥10 tahun, memiliki gejala demam ≥ 5 hari, memiliki gejala sakit perut, bersedia mengikuti penelitian. Hasil mikroskopis pewarnaan Gram dianalisis dengan aplikasi epicalc. Hasil mikroskopis pewarnaan Gram buffy coat yaitu ditemukan batang Gram negatif sebanyak 7 (26%) dan yang tidak ditemukan batang Gram negatif sebanyak 20 (74%). Hasil kultur penelitian ini terdapat pertumbuhan bakteri Gram negatif sebanyak 6 (22%) dan tidak terdapat pertumbuhan sebanyak 21 (78%). Kesimpulan hasil mikroskopis pewarnaan Gram buffy coat mempunyai nilai sensitifitas 67% dan spesifisitas 86%. Kata kunci : Pewarnaan Gram, Buffy Coat, Bakteremia
Staphylococcus aureus and Methicillin resistant Staphylococcus aureus From The Diabetic Ulcer Lutpiatina, Leka; Eriana, Nisa Nur Agistni
Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Infections of diabetic ulcers may occur because patients with diabetes mellitus have a weak immune system and the presence of high blood sugar into a strategic place for bacterial growth one of them is Staphylococcus aureus. Increased resistance of Staphylococcus aureus to various antibiotics, causing problems for the treatment of this infection. As by Methicillin-resistant Staphylococcus aureus (MRSA) This study aims to assess the presence of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) at diabetic ulcer patient at Banjarmasin city hospital. This research uses descriptive observational research type. The study sample was patients with grade 2 and three diabetic ulcers with a total of 30 samples. The result of the research found 14 Staphylococcus aureus bacteria, Staphylococcus epidermidis nine, Staphylococcus saprophyticus two and 5 Gram-negative bacteria. Of the 14 samples of Staphylococcus aureus, there were eight samples of cefoxitin-resistant samples. The conclusion was 47% of Staphylococcus aureus in patients with a diabetic ulcer at Banjarmasin City Hospital. There was a 57% Methicillin-resistant Staphylococcus aureus in the isolated Staphylococcus aureus found. Need further research on the resistance of antibiotics oxacillin and cefoxitin to other Staphylococcus sp bacteria derived from patient diabetic ulcers.
CEMARAN Staphylococcus aureus DAN Pseudomonas aerogenosa PADA STETESKOP DIRUMAH SAKIT lutpiatina, leka
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Medical devices that are susceptible to bacteria contaminated one of them is a stethoscope. Nosocomial infections that are problematic in the hospital include Staphylococcus aureus and Pseudomonas aerogenosa. These bacteria can cause mild skin infections to serious life-threatening infections. The purpose of this research is to know the existence of bacteria contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop at Banjarbaru hospital. This research uses descriptive survey method, which is a research method to describe objectively based on facts found. The samples were stethoscope in child care room, internal medicine room, delivery room, post partum room, perinatalogi room, and post-surgical treatment room at Banjarbaru hospital for 30 stethoscopes. The result of the research was found contamination of Staphylococcus aureus and Pseudomonas aerogenosa on steteskop. The stethome condition of the research data was 66.7% cleaned daily, the storage method was placed on the table 70% and the duration of using the stethome more than 1 year as much as 70%. The conclusion of stethoscope at Banjarbaru Hospital was contaminated with Staphylococcus aureus by 70% and Pseudomonas aerogenosa by 17%. Suggestion of research can be continued by knowing the existence of Staphylococcus aureus resistant antibiotic and Pseudomonas aerogenous antibiotic resistant at steteskop at Hospital
DAYA HAMBAT EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus aureus Lutpiatina, Leka; Amaliah, Nur Rizqi; Dwiyanti, Ratih Dewi
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

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Abstract

Daun kenikir (Cosmos caudatus Kunth.) sudah lama dimanfaatkan masyarakat untuk dikonsumsi maupun pengobatan tradisional. Daun kenikir memiliki kandungan fenol, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal dan konsentrasi bunuh minimal ekstrak daun kenikir terhadap bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat true eksperiment dengan rancangan postest only control group design. Variabel bebas dalam penelitian ini adalah konsentrasi ekstrak daun kenikir (Cosmos caudatus Kunth.).  Variabel terikat dalam penelitian ini adalah bakteri Staphylococcus aureus. Hasil penelitian Konsentrasi Hambat Minimal (KHM) menunjukkan kekeruhan pada konsentrasi 160 mg/ml, 320 mg/ml dan adanya kejernihan pada konsentrasi 340 mg/ml, 380 mg/ml dan 400 mg/ml. Hasil penelitian Konsentrasi Bunuh Minimal (KBM) diperoleh jumlah koloni pada konsentrasi 160 mg/ml sebanyak 60 CFU/ml, 320 mg/ml sebanyak 12 CFU/ml, 340 mg/ml sebanyak 3 CFU/ml, 380 mg/ml sebanyak 0 CFU/ml dan 400 mg/ml sebanyak 0 CFU/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa KHM ekstrak daun kenikir adalah 340 mg/ml dan KBM ekstrak daun kenikir adalah 380 mg/ml. Disarankan dilakukan penelitian lebih lanjut mengenai pengaruh ekstrak daun kenikir terhadap bakteri Staphylococcus aureus adalah dengan menggunakan pelarut lain.
Lactic Acid Levels Yogurt Red Beans with Addition of Honey Trigona sp Widyati, Ruri; Lutpiatina, Leka; Oktiyani, Neni; Haitami, Haitami
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

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Abstract

Red beans (Phaseolus vulgaris L.) are grains that are rich in nutrients and useful as a substitute for vegetable milk which can be processed into yogurt using Streptococcus thermophillus and Lactobacillus bulgaricus. Carbohydrates in red beans consist of a group of oligosaccharides that have little ability as an energy source for bacteria to produce lactic acid. Therefore, the making of red bean yogurt needs another source of sugar by adding Trigona sp. Honey. The purpose of this study was to determine the effect of adding Trigona sp honey by 0%, 1.9%, 3.8%, 5.7%, 7.6%, 9.5% to the total level of lactic acid in red bean yogurt incubated for 24 hours at 37oC. This type of research is an experiment with the Postest Only With Control Group Design research design using the alkalimetry titration method. The sampling technique used was purposive sampling technique, namely Trigona sp honey bee taken at Trigona sp bee farm in Tambangan, Tanah Laut. Indonesia. The results showed that there was an effect of adding Trigona sp honey to total lactic acid levels in red bean yogurt with the addition of Trigona sp honey 1.9% - 9.5% fulfilling the requirements of SNI 01.2981-2009. The highest average value of total lactic acid in red bean yogurt with the addition of Trigona sp honey 9.5% which is equal to 0.6672%. It is recommended for future researchers to use other types of carbohydrate sources and additives in the form of proteins. For the community, it is recommended to use alternative sources of other sugar and optimize the addition of Trigona sp. Honey.
Erythrocyte Morphology of Tuberculosis Patients Rifa'i, Ahmad; Muhlisin, Ahmad; Lutpiatina, Leka
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

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Abstract

Tuberculosis is one of the causes of anemia in chronic diseases in addition to rheumatoid arthritis, urserative colitis, Crohn's disease, ovarian malignancy and Hodgkin's lymphoma. Chronic disease anemia also known as anemia due to inflammation, occurs in patients with chronic infections. This anemia also occurs in some patients with malignancy. The causes of anemia are based on cell size, namely iron deficiency (often), anemia of chronic diseases (often), Thalassemia (often in certain ethnicities) causing the size of cells to become microsytic. Cells turn macrocytic due to liver disease, excess alcohol, megaloblastic anemia (vitamin B12 deficiency or folate or exposure to certain drugs), hemolysis, and aplastic anemia. Whereas the normal cell factors are blood loss, the initial phase of iron deficiency, anemia, chronic disease, and kidney failure. The aim of this study was to determine the type of anemia based on the morphological description of erythrocytes in the form, color, and size of erythrocytes seen in the smear blood supply of tuberculosis patients based on treatment duration of 0-6 months. The type of research used was a descriptive survey with a population of 15 people taken by accidental sampling. Data collection is done by examining the blood smear smears. Based on the examination of peripheral blood smear preparations in pulmonary tuberculosis patients, the morphology of erythrocytes in the form of Normocytic normochrome was 74% and microsocytic normochrome was 26%. Suggestions in the research for further research can be carried out with support other than the examination of peripheral blood smear.
Dengue Hemorrhagic Fever Vector in the Paring Sungai Martapura Indonesia Triyanti, Arista; Lutpiatina, Leka; Rifqoh, Rifqoh
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

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Abstract

Dengue Hemorrhagic Fever (DHF) is one of the public health problems that is still found in Indonesia. This disease always occurs repeatedly due to failure of vector control. Indonesia is the second largest country among 30 countries endemic to DHF. This disease can cause death especially in children. In Sungai Paring Village, January-December 2017, 2 cases of DHF were found. The purpose of this study was to find out the House Index (HI), Countainer Index (CI), Breteau Index (BI), larva free numbers (LFN) , Density Figure (DF) and larvae positive container types. This research is a type of descriptive survey research with total sampling technique. The population in this study were households and containers in the Paring Sungai Martapura Sub-District. The sample in this study was all water reservoirs in 100 respondent's houses. Of the 100 houses surveyed there were 40 positive larvae (HI 40%), 60% ABJ, 41 larvae positive containers from 356 examined containers (CI 11.52%) and positive larvae containers namely, ceramic bath 3 (0.84% ), 1 cement bath (0.28%), 18 used paint buckets (5.06%), 4 large bucket buckets (1.12%), 3 ablutions (0.84%) and 6 plastic drums (1.70%). Based on this research, it is expected that respondents and the community take precautionary measures and control of dengue hemorrhagic fever (DHF) vectors by 3M. For researchers to conduct further research to determine vector density fluctuations
Fungi That Produce Toxins in Salted Fish Susanti, Yuli; Lutpiatina, Leka; Dwiyanti, Ratih Dewi
Tropical Health and Medical Research Vol 1 No 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

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Abstract

Salted fish are fish that are processed through a process of salting and drying. The contamination of fungi in salted fish can be caused by prolonged storage. Storage of salted fish that is too long can cause the growth of various fungi. One of the fungi that often grows in salted fish is the fungus of Aspergillus sp. Some species of the Aspergillus sp fungi can produce aflatoxin, one of which is Aspergillus flavus. This study aims to determine the contamination of toxin-producing fungi in salted fish in the traditional Banjarbaru market in Indonesia. The type of research used is descriptive survey. Samples were taken by purposive sampling taken from 5 salted fish sellers each taken 3 different types of salted fish so that the number of samples was 15. The results were obtained from 15 samples examined, 6 positive samples contaminated with Aspergillus flavus fungi, 8 positive samples contaminated with Aspergillus fungi niger, 5 positive samples contaminated with Monilia sitophila fungi, 6 positive samples contaminated with Rhizopus sp fungi, 6 positive samples contaminated with Penicillium sp fungi, and 1 positive sample contaminated with Mucor sp fungi. Based on the results of the study, samples of salted fish contaminated with Aspergillus sp fungi were 73% (11 samples) and no samples were contaminated with Fusarium sp.
Sputum Quality of The Anytime And Outset for Examination Acid-Resistance Bacilli Lutpiatina, Leka; Wahidah, Wahidah; Nurhilaliah, Nurhilaliah; Rakhmina, Dinna; Rifqoh, Rifqoh
Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Diagnosis Tuberculosis (TB) can establish by microscopic examination of acid-resistant bacilli in the patient's sputum. Sputum quality greatly affects the diagnosis of TB, but the sputum collected by patients at community health centers sometimes does not meet the sputum quality requirements. The objective of this study was to assess the quality of sputum anytime and outset sputum on acid-resistant bacilli examination, from volume, color and viscosity parameters. The type of research used is descriptive observational. Respondents were suspected TB patients at the Marabahan and Aluh-aluh Public Health Centers of South Kalimantan province, Indonesia. Sputum samples taken were sputum anytime the first and outset sputum. The variables in this study are volume, color, and viscosity of sputum. The coloring of acid-resistant bacilli method Ziehl Nielsen. The results of the acid-resistant bacilli examination on sputum (86 specimens) found positive one (13%), positive three (2%), negative (85%). In morning sputum (86 specimens) found positive one (14%), positive three (2%), negative (84%). Sputum quality when with the good category as much as 36% and the quality of morning sputum with the good category as much as 55%. Conclusion Sputum outset has better quality than sputum at the anytime.
EFEKTIVITAS EKTRAK PROPOLIS LEBAH KELULUT (Trigona spp) DALAM MENGHAMBAT PERTUMBUHAN Salmonella typhi, Staphylococcus aureus DAN Candida albicans Lutpiatina, Leka
Jurnal Skala Kesehatan Vol 6 No 1 (2015): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

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Abstract

Propolis mempunyai khasiat sebagai antikanker, antivirus, antifungi dan antibiotika (Haryanto et al., 2012). Penelitian Agustina (2007), dihasilkan ekstrak propolis lebah asal Malang dapat mempengaruhi dan menghambat pertumbuhan bakteri gram positif Staphylococcus epidermidis pada konsentrasi 60% dan bakteri gram negatif Pseudomonas aeruginosa pada konsentrasi 70%. Penelitian mengenai efektivitas antibakteri dan anti jamur propolis dan lebah Trigona spp asal provinsi Kalimantan Selatan sejauh pengetahuan penulis belum pernah dilakukan. Tujuan umum penelitian ini untuk mengetahui konsentrasi yang efektif dari ekstrak propolis  lebah kelulut (Trigona spp) dalam menghambat pertumbuhan bakteri Salmonella typhi Staphylococcus aureus dan Candida albicans. Hipotesis penelitian adalah Ekstrak propolis lebah kelulut (Trigona spp) efektif dalam menghambat pertumbuhan bakteri Salmonella typhi, Staphylococcus aureus dan Candida albicans. Jenis penelitian yang digunakan dalam penelitian ini adalah metode true eksperiment berupa rancangan-rancangan eksperimen sungguhan dengan menggunakan  rancangan Postest Only Control Group Design (Notoatmodjo, 2010). Bahan penelitian adalah propolis dari sarang lebah kelulut (Trigona spp) yang ada di daerah Banjarbaru, dengan kriteria sarang yang bertekstur rapuh dan berwarna gelap. Data yang diperoleh ditabulasikan dan dilakukan analisis secara statistik dengan one way anova dan Kruskal Wallis α 0,05 pada tingkat kepercayaan 95%. Hasil penelitian konsentrasi yang efektif dari ekstrak propolis  untuk Salmonella typhi dan Staphylococcus aureus adalah 100% dan  tidak terdapat zone hambat untuk Candida albicans. Saran perlu dilakukan uji aktivitas antimikroba propolis lebah kelulut (Trigona spp) asal daerah Kalimantan Selatan terhadap candida albicans dengan metode dilusi. Kata Kunci : Ekstrak Propolis, Trigona spp, Salmonella typhi, Staphylococcus aureus, Candida albicans