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Efektivitas Berbagai Konsentrasi Kacang Kedelai (Glycine max (L.) Merill) Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans Nuryati, Anik; Huwaina, Ahsanul Dian
Jurnal Teknologi Laboratorium Vol 5 No 1 (2016): 2016 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Infeksi jamur kulit cukup banyak ditemukan di Indonesia, salah satu disebabkan oleh Candida albicans yaitu kandidiasis.Penyakit ini dapat diperiksa secara makroskopis dengan media SDA yang harganya cukup mahal, higroskopis dan tidak diperoleh sembarang tempat sehingga perlu dibuat media alternatif salah satunya yaitu menggunakan kacang kedelai. Penelitian ini bertujuan untuk mengetahui efektivitas berbagai konsentrasi kacang kedelai (Glycine max (L.) Merill) sebagai media alternatif terhadap pertumbuhan jamur Candida albicans. Jenis penelitian ini adalah penelitian eksperimen atau percobaan dengan post test with control group.Penelitian ini dilakukan pada bulan Februari 2015 di Laboratorium Mikrobiologi Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta. Obyek penelitian ini adalah kacang kedelai konsentrasi 2%, 4%, 6% dan 8% yang diujikan terhadap Candida albicans sebagai subyek penelitian. Media SDA (Sabouraud Dextrose Agar) digunakan sebagai kontrol terhadap pertumbuhan jamur Candida albicans. Pengolahan data yang digunakan meliputi uji deskriptif dan uji efektivitas. Hasil yang diperoleh diuji dengan uji deskriptif, didapatkan rata-rata koloni pada media kacang kedelai konsentrasi 2%, 4%, 6% dan 8% yaitu sebesar 3,67 koloni, 4,83 koloni, 5,17 kolonidan 5,33 koloni. Hasil uji efektivitas menunjukkan bahwa kacang kedelai dapat digunakan sebagai media alternative terhadap pertumbuhan jamur Candida albicans pada konsentrasi minimal 4% dengan hasil persentase sebesar 107,33%.Semakin tinggi konsentrasi kacang kedelai semakin banyak jumlah koloni yang dihasilkan
Uji Antibakteri Ekstrak Metanol Kulit Buah Manggis (garcinia mangostana l.) Terhadap Staphyllococcus aureus dan Escherechia coli Sujono, Sujono; Nuryati, Anik
Jurnal Teknologi Laboratorium Vol 6 No 1 (2017): 2017 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.336 KB) | DOI: 10.29238/teknolabjournal.v6i1.60

Abstract

Alfa mangostin, gamma-mangostin and xanthone group compounds are secondary metabolites contained in mangosteen (G. mangostana L.) which can be isolated from the fruit, bark, leaves and rind of mangosteen. All three compounds were shown to inhibit stronger against Mycobacterium tuberculosis. Alfa mangostin also active against Staphylococcus aureus and Enterococcus bacteria that are resistant to vancomisin and methicillin. Objective of this study to determine the antibacterial activity of methanol extract of mangosteen rind against Staphyllococcus aureus and Escherechia coli in vitro. Antibacterial activity test used is the diffusion wells and paper disc diffusion method. The test results obtained antibacterial activity of methanol extract of mangosteen rind can inhibit the growth of Staphyllococcus aureus at a concentration of 16%, but below its resistance zone diameter Cyprofloxacin. As against Escherichia coli, the methanol extract of mangosteen rind could not inhibit the growth of bacteria.
PENGGUNAAN BERBAGAI KONSENTRASI EKSTRAK ETHANOL DAUN RAMBUTAN (Nephelium lappaceum L.) TERHADAP MORTALITAS LARVA Culex quinquefasciatus Lestari, Dwi; Nuryati, Anik; Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 3 No 1 (2014): Maret 2014
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Mosquitoes are insects which are responsible in transmission process of many diseases in humans and animals. One type that is often found is Culex quinquefasciatus. Culex quinquefasciatus mosquitoes serve as vectors of disease filariasis and chikungunya. the mosquitos prevention that uses synthetic insecticide affects the negative side for the environment. Natural insecticide is the safe way that can be used as insecticide.Rambutan’s leaves contain compounds of saponin and tanin, it be used for larvacide’s.This research aims to find out about ethanol extract of rambutans leaves that can be used for larvacide, the impact, the influence of ethanol extract of rambutans leaves towards larva mortality and the effective concentrate in the researchThis research is quasi experimental with Post Test Control Group Design. The concentrate extract of rambutans leaves that is used is 0.025%, 0.05%, 0.1%, 0.2%, 0.4%,and 0.8% with duration 24 hours. Then, it is counted the amount of dead larvae. Then, the result is analyzed statically using Anova One Way and linear regression.The result showed that the percentage of the death of larva Culex quinquefasciatus in concentrate 0.025%, 0.05%, 0.1%,0.2%,0.4%, and 0.8% each of them are 77%,86%,87%,95%,98%,and 100%. The result of Anova One Way showed that the significant value is 0.000 (<0.05) which means that there is an impact of using rambutans leaves extract towards larva mortality Culex quinquefasciatus.The use of Ethanol extract can cause the mortality of larva Culex quinquefasciatus.
Pengaruh Simplisia Bunga Kenanga (Cananga odorata (Lamk). Hook.) sebagai Mat Elektrik Terhadap Knock-Down Nyamuk Aedes Aegypti nuryati, anik; nuryani, siti; wibowo, Andreaddo doni
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.463 KB) | DOI: 10.31964/mltj.v1i1.6

Abstract

Abstract: The main vector of dengue fever is Aedes aegypti. Ylang flower can be useful as an anti-mosquito, so in this study ylang flower botanicals chosen as rechargeable electric mat as an anti-mosquito Aedes aegypti. Objectives Determine the effect of various heavy and heavy crude drugs effective of ylang flowers (Cananga odorata (lamk.) Hook.) as an electric mat, the number of Aedes aegypti percentage knock-down of various heavy crude drugs ylang flowers. Methods This research uses experimental research with Post test with control. The results obtained from counting the number of knock-down mosquitoes Aedes aegypti after 1 hour exposure to various heavy simplisia ylang flowers are 1 g, 1,5 g, 2 g, 2,5 g, 3 g and 3,5 g. Results Ylang flowers as electricalmat having a knock-down effect on the mosquito Aedes aegypti. The mean effect of knock-down mosquitoes produced heavy crude drugs in 1 g, 1,5 g, 2 g, 2,5 g, 3 g and 3,5 g of 2,0, 2,75, 4,75, 6,0, 7,75 and 9,0. Conclusion Ylang flowers simplicia as electric mat influence 90.6% of the knock-down mosquito Aedes aegypti. The mean effect of knock-down mosquitoes produced heavy crude drugs in 1 g, 1.5 g, 2 g. 2.5 g, 3 g and 3.5 g of 2.0, 2.75, 4.75; 6.0; 7.75 and 9.0 and obtained the effective weight of 3.5 grams. The percentage knock-down mosquitoes in heavy crude drugs ylang flowers 1 g, 1.5 g, 2 g, 2.5 g, 3 g, and 3.5 g were 10%, 13.75%, 23.75%, 30%, 38.75%, and 45% Keywords Ylang flowers. Simplicia. Electric mat. Knock-down of Aedes aegypti. Abstrak: Vektor utama penyakit DBD adalah nyamuk Aedes aegypti. Bunga kenanga bermanfaat sebagai anti- nyamuk, simplisia bunga kenanga dipilih sebagai isi ulang mat elektrik sebagai anti-nyamuk Aedes aegpti. Tujuan penelitian ini mengetahui pengaruh dan berat efektif berbagai berat simplisia bunga kenanga (Cananga odorata (lamk).Hook) sebagai mat elektrik , jumlah nyamuk Aedes aegypti yang knock-down dan presentase knock-down dari berbagai berat simplisia bunga kenanga. Penelitian ini menggunakan jenis penelitian eksperimen dengan pendekatan Post test with kontrol. Hasil penelitian diperoleh dari penghitungan jumlah knock-down nyamuk Aedes aegypti setelah pemaparan 1 jam dengan berbagai berat simplisa bunga kenanga yaitu 1 g, 1,5 g, 2 g, 2,5 g,3 g dan 3,5 g. Penelitian ini dianalisis secara deskriptif dan uji statistik regresi linier. Hasil penelitian Simplisia bunga kenanga sebagai mat elektrik memiliki efek knock-down pada nyamuk Aedes aegypti. Rerata efek knock-down nyamuk yang dihasilkan simplisia pada berat 1 g, 1,5 g, 2 g. 2,5 g, 3 g dan 3,5 g sebesar 2,0; 2,75; 4,75; 6,0; 7,75 dan 9,0. Kesimpulan Simplisia bunga kenanga sebagai mat elektrik memberikan pengaruh 90,6% terhadap knock-down nyamuk Aedes aegypti. Rerata efek knock-down nyamuk yang dihasilkan simplisia pada berat 1 g, 1,5 g, 2 g. 2,5 g, 3 g dan 3,5 g sebesar 2,0; 2,75; 4,75; 6,0; 7,75 dan 9,0 dan didapatkan berat efektif yaitu 3,5 gram. Besarnya prosentase knock-down nyamuk pada berat simplisia bunga kenanga 1 g, 1,5 g, 2 g, 2,5 g,3 g, dan 3,5 g adalah sebesar 10 %, 13,75 %, 23,75 %, 30 %, 38,75 %, dan 45 %. Kata Kunci Bunga kenanga. Simplisia. Mat elektrik. Knock-down Aedes aegypti.
PENGARUH PEMBERIAN JUS BUAH SIRSAK (Annona muricata L.) TERHADAP KADAR TRIGLISERIDA PADA TIKUS PUTIH (Rattus norvegicus) HIPERLIPIDEMIA Wiraditya, Nur Eka; Nuryati, Anik; Rahayu, Muji
Jurnal Analis Medika Biosains (JAMBS) Vol 1, No 2 (2014): Jurnal Analis Medika Bio Sains
Publisher : Jurusan Analis Kesehatan, Poltekkes Kemenkes Mataram

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Abstract

AbstrakKadar trigliserida yang tinggi adalah salah satu penyebab Penyakit Jantung Koroner (PJK). Peningkatan diakibatkan kadar trigliserida dalam darah naik dan menyebabkan atherosklerosis. Usaha untuk menurunkan resiko PJK dilakukan penelitian menggunakan buah-buahan yang dapat menurunkan kadar trigliserida. Salah satu buah yang diteliti adalah buah sirsak (Annona muricata L.) memiliki zat kimia alami yang diduga dapat menurunkan kadar lemak atau antihiperlipidemia. Kandungan yang ada pada buah sirsak antara lain flavonoid dan niasin. Telah dilakukan penelitian untuk mengetahui seberapa besar pengaruh jus buah sirsak terhadap kadar trigliserida. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian jus buah sirsak (Annona muricata L.) terhadap kadar trigliserida pada tikus putih (Rattus norvegicus) hiperlipidemia, presentase penurunan dan besar pengaruh jus buah sirsak. Desain penelitian ini adalah pre and post test with control group design, menggunakan lima kelompok, jumlah total tikus putih adalah 25 ekor dengan rincian untuk masing – masing kelompok adalah lima ekor. Pengambilan darah melalui sinus orbitalis dan diperiksa kadar trigliseridanya. Data yang diperoleh dianalisis secara deskriptif dan statistik menggunakan uji Anova One Way dan uji Correlation. Presentase penurunan kadar trigliserida tikus putih yang diberi jus buah pada K(1), K(2) dan K(3) adalah 46,27%, 53,06% dan 60,04%. Besar pengaruh jus buah sirsak dengan kadar trigliserida adalah 79,6%. Hasil uji Anova One Way menunjukkan signifikan 0,000 yang artinya ada pengaruh pemberian jus buah sirsak terhadap kadar trigliserida pada tikus putih. Hasil uji Correlation adalah terdapat hubungan yang kuat antara jus buah sirsak terhadap kadar trigliserida tikus putih.Kata Kunci : Hiperlipidemia, Jus buah sirsak, Kadar trigliserida, Tikus putih.
Perbedaan Jumlah Trombosit Pada Darah EDTA Yang Segera Diperiksa dan Penundaan Selama 1 Jam di Laboratorium RSJ Grhasia Yogyakarta sujud, sujud; hardiasari, ratih; nuryati, anik
Medical Laboratory Technology Journal Vol 1, No 2 (2015): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.628 KB) | DOI: 10.31964/mltj.v1i2.21

Abstract

Pre-analytical phases is a very important stage and need to be considered properly. Pre-analytical phases of which is the process of blood sampling, sample delivery, the inclusion of the type of inspection, sample preparation and selection tools. A fact which still often the case that their neglect by nurses or laboratory personnel in taking and processing the blood samples. Blood samples for examination platelet counts as much as possible is done properly and the sample must be examined in less than 1 hour after taking blood. Delays checks can cause a decrease in platelet count. Delays often occur for over an hour due to the shipment of samples from wards that are not immediately performed or work shift lab personnel. The aim of research to determine the difference in the number of platelets in the blood EDTA is immediately checked and a delay of 1 hour by using KX-21 Hematology analyzer. Experimental study design with pre- and post-test study without control. The study was conducted at the Laboratory of RSJ Grhasia Yogyakarta, with the object of research is venous blood from patients RSJ Grhasia between the ages of 20-50 years of both men - men and women. Data were analyzed parametric statistical tests Paired samples t-test. The results of significant research value of the results of parametric statistical tests Paired samples t-test was 0,000 (sig
Efektifitas Pemberian Perasan Bawang Putih (Allium Sativum Linn) Dosis Tunggal Terhadap Jumlah Telur Cacing Gelang (Toxocara Canis) Secara In Vivo Utami, Ratri Risky; Nuryati, Anik; Nuryani, Siti
Jurnal Teknologi Laboratorium Vol 3 No 2 (2014): 2014 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

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Abstract

Background this research is worm disease problematic in the field of health. Treatment for the disease worms that have been used are synthetic drugs that have side effects are not good for health and can lead to dependence. One of the medicinal plants used for deworming is garlic. Chemical constituents in garlic is allicin that is anthelmintics.To prove that it is carried out in vivo study using dogs infected with Toxocara canis worms as test animals.Objective this research to knowing of the effectiveness of various doses of garlic juice with a gift on the first day against Toxocara canis worm egg counts in vivo and the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration of juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation.Methods this research is an experimental study using dogs infected with Toxocara canis nearly the same number of eggs as well as the research design pretest-posttest with control group.Results the largest decrease in the effectiveness of Toxocara canis worm egg counts after administration juice garlic at a dose of 0,5; 1 and 1,5 percent weight within 6 days of observation known to occur on day 3 with a percentage of effectiveness are 48,49%; 67,18%; dan 82,85%. The higher doses of garlic juice, the more effective to decline in the amount of worm eggs Toxocara canis in vivo.Conclusion this research is garlic juice with various doses are not as effective as once treatment in reducing the the amount of eggs bracelet worm (Toxocara canis) in vivo.